浙江农业学报 ›› 2016, Vol. 28 ›› Issue (9): 1462-1475.DOI: 10.3969/j.issn.1004-1524.2016.09.03

• 作物科学 • 上一篇    下一篇

广丰千金薯驯化移栽苗对PEG干旱胁迫的光合生理响应

柯维忠, 王丽, 杨星鹏, 徐玉琴, 吴丹, 江霞, 夏瑾华, 尹明华, 洪森荣*   

  1. 上饶师范学院 生命科学学院,江西 上饶 334001
  • 收稿日期:2015-12-28 出版日期:2016-09-15 发布日期:2016-11-23
  • 通讯作者: 洪森荣,E-mail: hongsenrong@163.com
  • 作者简介:柯维忠(1976—),男,江西上饶人,实验师,主要从事植物生理学研究。E-mail: keweizhong1976@163.com
  • 基金资助:
    江西省教育厅2014年度科学技术研究一般项目(GJJ14712); 2015年江西省高等学校大学生创新训练计划项目(201510416007)

Photosynthetic physiological responses of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress

KE Wei-zhong, WANG Li, YANG Xing-peng, XU Yu-qin, WU Dan, JAING Xia, XIA Jin-hua, YIN Ming-hua, HONG Sen-rong*   

  1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, China
  • Received:2015-12-28 Online:2016-09-15 Published:2016-11-23

摘要: 为了探究广丰千金薯驯化移栽苗的耐旱生理机制,测定了干旱胁迫下广丰千金薯驯化移栽苗的多项光合生理指标。结果表明,50和100 g·L-1 PEG-6000可显著促进广丰千金薯移栽驯化苗的生物量和根冠比,而150和200 g·L-1 PEG-6000则显著减少其生物量和根冠比。当PEG-6000浓度为50和100 g·L-1,其叶片相对含水量、气孔开度、Tr、Fo、Fv/Fm、ΦPSⅡNPQ和ZR含量无显著变化,叶绿素含量、气孔密度、Pn、Gs、Ls、WUECUEFm、Fv/Fo、Fv'/Fm'、qP、GA3和ABA含量显著增加,Ci显著下降。当PEG-6000浓度达到150和200 g·L-1,其叶片相对含水量、叶绿素含量、气孔开度、Pn、Gs、Ls、Tr、WUECUEFo、Fm、Fv/Fo、ΦPSⅡFv'/Fm'、Fv/Fm、qP、GA3、ZR和IAA显著下降,气孔密度、Ci、NPQ和ABA含量显著增加。在干旱胁迫下,广丰千金薯移栽驯化苗叶片ZR/ABA和ZR/IAA随着PEG-6000浓度的增大而显著下降,而ABA/(IAA+GA3+ZR)则随PEG-6000浓度的增大而显著提高。在干旱胁迫下广丰千金薯移栽驯化苗叶片倍性稳定,但当PEG-6000浓度达到200 g·L-1,电泳图谱上出现了明显的DNA ladder。因此,广丰千金薯驯化移栽苗能适应一定程度的干旱胁迫,但随着干旱胁迫的加剧,会影响其生长发育、光合作用和内源激素的调控机制,导致细胞程序性死亡。

关键词: 广丰千金薯, 驯化移栽苗, 干旱胁迫, 光合生理响应

Abstract: In order to explore the drought-resistant physiological mechanism of Dioscorea opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets, its photosynthetic physiological responses under drought stress were tested in this paper. The results showed that 50 and 100 g·L-1 PEG-6000 could significantly promote the biomass and root-shoot ratio of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets, while 150 and 200 g·L-1 PEG-6000 could significantly reduced the biomass and root-shoot ratio. When PEG-6000 concentration was 50 and 100 g·L-1, the leaf relative water content, stomatal aperture, Tr, Fo, Fv/Fm, ΦPSⅡ, NPQ and ZR had no significant changes, the chlorophyll content, stomatal density, Pn, Gs, Ls, WUE, CUE, Fm, Fv/Fo, Fv'/Fm', IAA, GA3 and ABA increased significantly, Ci decreased significantly. When PEG-6000 concentration reached 150 and 200 g·L-1, the leaf relative water content, chlorophyll content, stomatal aperture, Pn, Gs, Ci, Tr, WUE, CUE, Fo, Fm, Fv/Fo, ΦPSⅡ, Fv'/Fm', Fv/Fm, qP, GA3, ZR and IAA decreased significantly, stomatal density, Ls, NPQ and ABA increased significantly. Under drought stress, ZR/ABA ratio and ZR/IAA ratio of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets decreased significantly with the increase of PEG-6000 concentration, while the ABA/ (IAA+GA3+ZR) increased significantly with the increase of PEG-6000 concentration. The FCM test results of DNA content showed that the ploidy of D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets under PEG drought stress was stable, but when PEG-6000 concentration reached 200 g·L-1, there were clear DNA ladders in the electrophoretic pattern. The results showed that D. opposite Thunb. cv. Guangfeng Qianjin acclimated and transplanted plantlets could adapt to a certain degree of drought stress, but with the aggravation of drought stress, its growth and development, photosynthesis and endogenous hormone regulation mechanism would be affected, which would lead to programmed cell death.

Key words: Dioscorea opposite Thunb. cv. Guangfeng Qianjin, acclimated and transplanted plantlets, drought stress, photosynthetic physiological responses

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