鸡生长发育早期骨骼肌IGF1R mRNA表达规律研究

doi:10.3969/j.issn.1004-1524.2020.07.04

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (7): 1160-1165.DOI: 10.3969/j.issn.1004-1524.2020.07.04

鸡生长发育早期骨骼肌IGF1R mRNA表达规律研究

盛中伟, 姬改革, 刘一帆, 巨晓军, 单艳菊, 邹剑敏, 章明, 屠云洁, 束婧婷^*

江苏省家禽科学研究所,江苏扬州 225125

收稿日期:2019-09-30 出版日期:2020-07-25 发布日期:2020-07-28
通讯作者: * 束婧婷,E-mail:shutingting@163.com
作者简介:盛中伟(1987—),男,江苏扬州人,硕士,助理研究员,研究方向为家禽遗传育种研究。E-mail:shengzw@yeah.net
基金资助:
国家自然科学基金(31572358); 江苏现代农业(肉鸡)产业技术体系(JATS〔2018〕249); 江苏省农业重大新品种创制项目(PZCZ201728); 江苏省农业自主创新基金项目(CX(18)3003); 现代农业产业技术体系建设专项(CARS-41); 江苏省属公益类科研院所自主科研经费(BM2018026)

Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development

SHENG Zhongwei, JI Gaige, LIU Yifan, JU Xiaojun, SHAN Yanju, ZOU Jianmin, ZHANG Ming, TU Yunjie, SHU Jingting^*

Jiangsu Institute of Poultry Science,Yangzhou 225125,China

Received:2019-09-30 Online:2020-07-25 Published:2020-07-28

摘要/Abstract

摘要： 以生长速度不同的花山麻鸡和清远麻鸡为试验素材,采用实时荧光定量PCR方法检测鸡9、12、16、21胚龄(E9 d、E12 d、E16 d、E21 d)和出雏后7日龄(7 d)时胸肌和腿肌中IGF1R mRNA表达变化情况,并与肌肉质量进行相关性研究。结果发现,21胚龄时,花山麻鸡和清远麻鸡胸肌质量都出现了显著降低,腿肌质量持续增长。花山麻鸡胸肌IGF1R mRNA表达呈前高后低趋势,9胚龄时表达量最高,之后显著降低并维持在较低的水平,出雏后7日龄时表达量再次下降;清远麻鸡在胸肌中的表达则呈“波浪形”,9胚龄和16胚龄表达量升高,其他日龄表达量显著降低。腿肌中,花山麻鸡IGF1R mRNA表达量在16胚龄之后显著降低;清远麻鸡腿肌IGF1R mRNA表达呈依次递减模式,各时间点之间差异显著(P<0.05)。品种间各个时间点胸肌和腿肌IGF1R mRNA表达量均呈显著差异(P<0.05)。两个品种骨骼肌IGF1R mRNA表达与胸肌、腿肌质量均呈极显著相关(P<0.01)。以上结果初步揭示了生长发育早期不同品种鸡胸肌和腿肌IGF1R基因表达发育变化趋势和品种差异,为深入研究IGF1R基因在鸡肌肉发育中的调控机理提供基础资料。

关键词: 鸡, 骨骼肌, 早期发育, IGF1R基因, mRNA表达

Abstract: In the present study, the Huashan and Qingyuan partridge chickens differing in growth rates were used as experimental materials,fluorescent real-time quantitative PCR (RT-PCR) was used to detect the mRNA expression pattern of IGF1R gene in pectoral and leg muscle of chickens on the embryonic days of 9, 12, 16, 21 and 7 days post-hatching. The results showed that pectoral muscle weight significantly decreased in the two breeds at the embryonic days 21, but leg muscle weigh significantly increased as time going. IGF1R mRNA expression tendency of pectoral muscle in Huashan partridge chickens were from the high to the low: the embryonic days 9 was an expression peak, and then the expression level significantly decreased and kept a low level and down-regulated in 7 days post-hatching again. IGF1R mRNA expression of pectoral muscle in Qingyuan partridge chickens were a wavy trend: the expression levels of IGF1R gene on pectorals at 9 and 16 embryo age were higher, others were significant down-regulated. In leg muscle, the expression levels of IGF1R gene were significant down-regulated after 16 embryo age in Huashan partridge chicken; the expression levels of IGF1R gene in Qingyuan partridge chickens were decreased gradually and there were significant differences among groups(P<0.05). The expression level of IGF1R gene in pectoral and leg muscles were significantly different between varieties at different time(P<0.05). The expression level of IGF1R gene were significantly correlated with the weight of pectoral and leg muscles, respectively (P<0.01). The above results were preliminarily revealed the development changes and difference of IGF1R gene expression in pectoral and leg muscles during early development of different breeds, provide basic data for further study on regulation mechanism of chicken muscle development.

Key words: chicken, skeletal muscle, early development, IGF1R, mRNA expression

中图分类号:

S831

盛中伟, 姬改革, 刘一帆, 巨晓军, 单艳菊, 邹剑敏, 章明, 屠云洁, 束婧婷. 鸡生长发育早期骨骼肌IGF1R mRNA表达规律研究[J]. 浙江农业学报, 2020, 32(7): 1160-1165.

SHENG Zhongwei, JI Gaige, LIU Yifan, JU Xiaojun, SHAN Yanju, ZOU Jianmin, ZHANG Ming, TU Yunjie, SHU Jingting. Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development[J]. , 2020, 32(7): 1160-1165.

参考文献

[1] DENLEY A, COSGROVE L J, BOOKER G W, et al.Molecular interactions of the IGF system[J]. Cytokine & Growth Factor Reviews, 2005, 16(4/5):421-439.
[2] BAKER J, LIU J P, ROBERTSON E J, et al.Role of insulin-like growth factors in embryonic and postnatal growth[J]. Cell, 1993, 75(1):73-82.
[3] ZANOU N, GAILLY P.Skeletal muscle hypertrophy and regeneration: interplay between the myogenic regulatory factors (MRFs) and insulin-like growth factors (IGFs) pathways[J]. Cellular and Molecular Life Sciences, 2013, 70(21):4117-4130.
[4] SANEYASU T, TSUCHIHASHI T, KITASHIRO A, et al.The IGF-1/Akt/S6 pathway and expressions of glycolytic myosin heavy chain isoforms are upregulated in chicken skeletal muscle during the first week after hatching[J]. Animal Science Journal, 2017, 88(11):1779-1787.
[5] KOCAMIS H, KIRKPATRICK-KELLER D C, KLANDORF H, et al. In ovo administration of recombinant human insulin-like growth factor-I alters postnatal growth and development of the broiler chicken[J]. Poultry Science, 1998, 77(12):1913-1919.
[6] O'NEILL B T, LAURITZEN H P M M, HIRSHMAN M F, et al. Differential role of insulin/IGF-1 receptor signaling in muscle growth and glucose homeostasis[J]. Cell Reports, 2015, 11(8):1220-1235.
[7] CASTELA M, LINAY F, ROY E, et al.Igf1r signalling Acts on the anagen-to-catagen transition in the hair cycle[J]. Experimental Dermatology, 2017, 26(9):785-791.
[8] BENARROCH E E.Insulin-like growth factors in the brain and their potential clinical implications[J]. Neurology, 2012, 79(21):2148-2153.
[9] ZHANG M, XUAN S H, BOUXSEIN M L, et al.Osteoblast-specific knockout of the insulin-like growth factor (IGF) receptor gene reveals an essential role of IGF signaling in bone matrix mineralization[J]. The Journal of Biological Chemistry, 2002, 277(46):44005-44012.
[10] SELL C, DUMENIL G, DEVEAUD C, et al.Effect of a null mutation of the insulin-like growth factor I receptor gene on growth and transformation of mouse embryo fibroblasts[J]. Molecular and Cellular Biology, 1994, 14(6):3604-3612.
[11] CHENG Y Y, LIU S C, ZHANG X, et al.Expression profiles of IGF-1R gene and polymorphisms of its regulatory regions in different pig breeds[J]. The Protein Journal, 2016, 35(3):231-236.
[12] MOHAMMED R H, ANDERTON H, BRAMELD J M, et al.Effects of insulin like growth factors on early embryonic chick limb myogenesis[J]. PLoS One, 2017, 12(10):e0185775.
[13] DU M, YAN X, TONG J F, et al.Maternal obesity, inflammation, and fetal skeletal muscle development[J]. Biology of Reproduction, 2010, 82(1):4-12.
[14] PICARD B, LEFAUCHEUR L, BERRI C, et al.Muscle fibre ontogenesis in farm animal species[J]. Reproduction, Nutrition, Development, 2002, 42(5):415-431.
[15] 李玥, 袁丽霞, 杨晓静, 等. 早期和后期限饲对肉鸡腓肠肌肌纤维类型和生长轴相关基因表达的影响[J]. 动物学报, 2006, 52(6):1133-1141.
LI Y, YUAN L X, YANG X J, et al.Effects of early and late feed restriction on myofiber types and expression of growth-related genes in gastrocnemius of broiler chickens[J]. Acta Zoologica Sinica, 2006, 52(6):1133-1141.(in Chinese with English abstract)
[16] CHEN W, TANGARA M, XU J, et al.Developmental transition of pectoralis muscle from atrophy in late-term duck embryos to hypertrophy in neonates[J]. Experimental Physiology, 2012, 97(7):861-872.
[17] HU Y, LIU H X, SHAN Y J, et al.The relative expression levels of insulin-like growth factor 1 and myostatin mRNA in the asynchronous development of skeletal muscle in ducks during early development[J]. Gene, 2015, 567(2):235-243.
[18] 顾以韧, 张凯, 李明洲, 等. 猪背最长肌中胰岛素样生长因子(IGFs)系统基因的发育表达模式[J]. 遗传, 2009, 31(8):837-843.
GU Y R, ZHANG K, LI M Z, et al.Developmental expression changes of insulin-like growth factors (IGFs)system genes in longissimus dorsi muscle of two pig breeds[J]. Hereditas, 2009, 31(8):837-843. (in Chinese with English abstract)
[19] 朱春红, 徐文娟, 胡艳, 等. 高邮鸭和金定鸭发育早期骨骼肌发育及胰岛素样生长因子1受体基因(IGF-IR)表达分析[J]. 农业生物技术学报, 2013, 21(2):192-198.
ZHU C H, XU W J, HU Y, et al.Early development and developmental expression of insulin-like growth factor 1 receptor (IGF-IR) mRNA in Gaoyou duck and jinding duck (Anas platyrhynchos domestica) skeletal muscle[J]. Journal of Agricultural Biotechnology, 2013, 21(2):192-198.(in Chinese with English abstract)
[20] YU M L, WANG H, XU Y L, et al.Insulin-like growth factor-1 (IGF-1) promotes myoblast proliferation and skeletal muscle growth of embryonic chickens via the PI3K/Akt signalling pathway[J]. Cell Biology International, 2015, 39(8):910-922.
[21] LU F Z, WANG X X, PAN Q X, et al.Expression of genes involved in the somatotropic, thyrotropic, and corticotropic axes during development of langshan and Arbor acres chickens[J]. Poultry Science, 2008, 87(10):2087-2097.
[22] LIU Y L, GUO W, PU Z Y, et al.Developmental changes of Insulin-like growth factors in the liver and muscle of chick embryos[J]. Poultry Science, 2016, 95(6):1396-1402.
[23] 束婧婷, 朱文奇, 单艳菊, 等. 胰岛素样生长因子系统基因在不同鸭种发育早期肝和肌肉中的表达[J]. 畜牧兽医学报, 2014, 45(2):183-190.
SHU J T, ZHU W Q, SHAN Y J, et al.Expression of insulin-like growth factor system genes in liver and breast muscle tissue during early development of different duck breeds[J]. Acta Veterinaria et Zootechnica Sinica, 2014, 45(2):183-190. (in Chinese with English abstract)
[24] JIA J J, AHMED I, LIU L X, et al.Selection for growth rate and body size have altered the expression profiles of somatotropic Axis genes in chickens[J]. PLoS One, 2018, 13(4):e0195378.

鸡生长发育早期骨骼肌IGF1R mRNA表达规律研究

Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development

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