滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析

doi:10.3969/j.issn.1004-1524.2020.08.03

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (8): 1351-1356.DOI: 10.3969/j.issn.1004-1524.2020.08.03

滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析

王晓薇¹, 樊毅², 马小明¹, 马丽娜¹, 岳彩娟¹, 王锦¹, 赵正伟¹, 马青^1,*

1.宁夏农林科学院动物科学研究所,宁夏银川 750000;
2.银川中科元昊科技有限公司,宁夏银川 750000

收稿日期:2020-02-11 出版日期:2020-08-25 发布日期:2020-08-28
通讯作者: *,马青,E-mail:maqing1973@126.com
作者简介:王晓薇(1988—),女,宁夏银川人,硕士,助理研究员,研究方向为动物遗传育种。E-mail:wxw0812@126.com
基金资助:
宁夏农林科学院农业科技自主创新专项科技创新引导项目(NKYQ-18-01); 宁夏农林科学院农业科技自主创新引导项目(2017-NXKY05); 宁夏回族自治区育种专项(2018NYYZ04); 宁夏回族自治区科技基础条件建设计划—创新平台专项资金(DKS-18-05)

Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

WANG Xiaowei¹, FAN Yi², MA Xiaoming¹, MA Lina¹, YUE Caijuan¹, WANG Jin¹, ZHAO Zhengwei¹, MA Qing^1,*

1. Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750000, China;
2. Yinchuan Zhongke Yuanhao Technology Co., Ltd., Yinchuan 750000, China

Received:2020-02-11 Online:2020-08-25 Published:2020-08-28

摘要/Abstract

摘要： 为了探究影响哺乳动物毛色的黑色素皮质受体-1基因(MC1R)和转录因子25基因(TCF25)与滩羊毛色的关系,本研究利用实时荧光定量PCR(qRT-PCR)技术,对15只不同毛色滩羊(纯白、褐斑、黑斑)皮肤组织中MC1R基因和TCF25基因的mRNA表达量进行研究。结果显示,MC1R基因和TCF25基因在3种不同毛色滩羊中均有表达。MC1R基因在不同毛色滩羊皮肤组织中表达量为黑斑>褐斑>纯白,其中黑斑组极显著高于纯白组(P<0.01),显著高于褐斑组(P<0.05),褐斑组显著高于纯白组(P<0.05);TCF25基因在不同毛色滩羊皮肤组织中表达量为纯白>褐斑>黑斑,组间差异不显著(P>0.05)。试验表明了目的基因表达量与滩羊毛色间的关系,为今后滩羊毛色选育与品种改良提供参考。

关键词: 滩羊, 毛色, MC1R基因, TCF25基因

Abstract: In order to explore the relationship between the melanocortin-1-receptor gene (MC1R) and transcription factor 25 gene(TCF25), which affect the coat colors of mammals, and the coat colors of Tan sheep, real-time fluorescent quantitative PCR (qRT-PCR) technique was used to study the mRNA expression of MC1R gene and TCF25 gene in skin tissues of 15 Tan sheep with different coat colors (pure white, brown spot and black spot). The results showed that MC1R gene and TCF25 gene were expressed in three different kinds of coat colors of Tan sheep. Expression quantity of MC1R gene in skin tissues of different coat colors of Tan sheep was black spot>brown spot>pure white. The black spot group was significantly higher than the pure white group (P<0.01), the brown spot group was significantly higher than the brown spot group (P<0.05), and the brown spot group was significantly higher than the pure white group (P<0.05). The expression quantity of TCF25 gene in skin tissues of different coat colors of Tan sheep was pure white>brown spot>black spot, with no significant difference among groups (P>0.05).The results showed the relationship between the expression level of target gene and the coat colors of Tan sheep, and provided references for the color breeding and improvement of Tan sheep in the future.

Key words: Tan sheep, coat color, MC1R gene, TCF25 gene

中图分类号:

S818.9

王晓薇, 樊毅, 马小明, 马丽娜, 岳彩娟, 王锦, 赵正伟, 马青. 滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析[J]. 浙江农业学报, 2020, 32(8): 1351-1356.

WANG Xiaowei, FAN Yi, MA Xiaoming, MA Lina, YUE Caijuan, WANG Jin, ZHAO Zhengwei, MA Qing. Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25[J]. , 2020, 32(8): 1351-1356.

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滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析

Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

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