丁硫克百威在豇豆不同时期施用的降解代谢研究

doi:10.3969/j.issn.1004-1524.2020.11.16

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (11): 2050-2058.DOI: 10.3969/j.issn.1004-1524.2020.11.16

丁硫克百威在豇豆不同时期施用的降解代谢研究

王娣^1,2, 狄珊珊¹, 王新全¹, 张昌朋¹, 王祥云^1,*, 王萌^3,*

1.农产品质量安全危害因子与风险防控国家重点实验室(筹),农业农村部农药残留检测重点实验室,浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
2.海南大学园艺学院,海南海口 570228;
3.海南大学植物保护学院,海南海口 570228

收稿日期:2020-06-07 出版日期:2020-11-25 发布日期:2020-12-02
通讯作者: * 王祥云,E-mail: wangxiangyun2@sina.com;王萌,E-mail: wangmeng@hainanu.edu.cn
作者简介:王娣(1994—),女,山东济南人,硕士研究生,主要研究方向为农药残留。E-mail: dd_wangdi1008@163.com
基金资助:
公益性行业(农业)科研专项(201503107-12); 2019年度国家农产品质量安全风险评估项目(GJFP2019006); 2019年海南省高层次人才基金(2019RC058); 浙江省自然科学基金(LQ14B070004)

Degradation of carbosulfan after its application in different growth stage of cowpea

WANG Di^1,2, DI Shanshan¹, WANG Xinquan¹, ZHANG Changpeng¹, WANG Xiangyun^1,*, WANG Meng^3,*

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products (in Prepared), Key Laboratory for Pesticide Residue Detection, Ministry of Agriculture and Rural Affairs, Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Horticulture, Hainan University, Haikou 570228, China;
3. College of Plant Protection, Hainan University, Haikou 570228, China

Received:2020-06-07 Online:2020-11-25 Published:2020-12-02

摘要/Abstract

摘要： 为明确丁硫克百威在豇豆播种期、苗期、结荚期使用后的降解代谢,以及可能产生的膳食风险,分别以其在蔬菜上登记的最低剂量、最高剂量、最高剂量的1.5倍3种剂量施药,进行田间模拟残留试验。将采集的成熟豇豆通过乙腈提取、C18分散净化,经超高效液相色谱串联质谱方法检测,测定豇豆中丁硫克百威及其代谢物——克百威和3-羟基克百威的残留量。结果表明,丁硫克百威、克百威和3-羟基克百威在豇豆中的定量限均为0.01 mg·kg^-1,在0.01~1 mg·kg^-1的添加水平下,丁硫克百威、克百威和3-羟基克百威的平均回收率为72%~105%,相对标准偏差为1.4%~20.1%。丁硫克百威使用后的超标风险主要源于其代谢物——克百威和3-羟基克百威。播种期施药后的豇豆样品均无丁硫克百威及其代谢物检出;苗期以最高剂量的1.5倍施药后,第10天的样品中克百威(含3-羟基克百威)的残留值超出中国国家标准中规定的最大允许残留限量(MRL);结荚期2次或3次施药后7 d内克百威(含3-羟基克百威)的残留值超出MRL。结荚期施药时,丁硫克百威在2次施药和3次施药后的慢性膳食摄入风险和急性膳食摄入风险均较低,小于100%;但克百威(含3-羟基克百威)的急性膳食摄入风险较高,以最高剂量的1.5倍2次施药或3次施药后,克百威(含3-羟基克百威)的急性膳食摄入风险于药后7 d才降至100%以下。综上,播种期使用丁硫克百威不会导致豇豆中残留超标,可以安全使用;但苗期和结荚期使用丁硫克百威存在极高的风险,应禁止其在播种期以外的使用。

关键词: 豇豆, 丁硫克百威, 克百威, 3-羟基克百威, 农药残留, 风险评估

Abstract: The degradation and risk assessment of carbosulfan application in different growth stages of cowpea (sowing, seedling and podding) were investigated. In field experiments, carbosulfan EC (emulsifiable concentrate) was applied in the different growth stages of cowpea with different doses (low dose, high dose and 1.5-fold high dose according to the registration). Cowpea samples were collected at mature stage, which were extracted with acetonitrile and purified by C18. The residue levels of carbosulfan and its metabolites (carbofuran and 3-hydroxycarbofuran) were determined by LC-MS/MS (liquid chromatography-tandem mass spectrometry). The limit of quantification (LOQ) values of carbosulfan, carbofuran and 3-hydroxycarbofuran were all 0.01 mg·kg^-1. The average recoveries of carbosulfan and its metabolites in cowpea at 4 spiked levels (0.01, 0.05, 0.2, 1 mg·kg^-1) ranged from 72% to 105% with relative standard deviation (RSD) in the range of 1.4%-20.1%. The residue risk of carbosulfan was mainly due to its metabolites (carbofuran and 3-hydroxycarbofuran). When carbosulfan was applied at sowing stage of cowpea, the residue levels of carbosulfan and its metabolites were below LOQ. After application at 1.5-fold high dose in the seedling stage, the residue level of carbofuran (including 3-hydroxycarbofuran) in cowpea on the 10th day exceeded the maximum residue limit (MRL) in China. When carbosulfan was applied in the podding stage two or three times, the residue level of carbofuran (including 3-hydroxycarbofuran) in cowpea within 7 d exceeded the MRL. When carbosulfan was applied in the podding stage of cowpea, the risk of chronic dietary intake and acute dietary intake of carbosulfan was lower than 100%, but the risk of acute dietary intake of carbofuran (including 3-hydroxycarbofuran) was higher than 100% within 7 d. Therefore, the application of carbosulfan in the sowing stage of cowpea was recommended, while application in the seedling and podding stage existed high risk, which should be forbiden.

Key words: cowpea, carbosulfan, carbofuran, 3-hydroxycarbofuran, pesticide residue, risk assessment

中图分类号:

王娣, 狄珊珊, 王新全, 张昌朋, 王祥云, 王萌. 丁硫克百威在豇豆不同时期施用的降解代谢研究[J]. 浙江农业学报, 2020, 32(11): 2050-2058.

WANG Di, DI Shanshan, WANG Xinquan, ZHANG Changpeng, WANG Xiangyun, WANG Meng. Degradation of carbosulfan after its application in different growth stage of cowpea[J]. , 2020, 32(11): 2050-2058.

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丁硫克百威在豇豆不同时期施用的降解代谢研究

Degradation of carbosulfan after its application in different growth stage of cowpea

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