转Cry抗虫基因玉米对家蚕的安全性评价

doi:10.3969/j.issn.1004-1524.2020.11.15

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (11): 2042-2049.DOI: 10.3969/j.issn.1004-1524.2020.11.15

转Cry抗虫基因玉米对家蚕的安全性评价

姜媛媛^1,2, 纪艺^1,2, 来勇敏¹, 陈笑芸¹, 徐俊锋¹, 徐晓丽^1,*, 马莲菊^2,*

1.农产品质量安全危害因子与风险防控国家重点实验室(筹),浙江省农业科学院农产品质量标准研究所,浙江杭州310021;
2.沈阳师范大学生命科学学院,辽宁沈阳 110034

收稿日期:2020-03-24 出版日期:2020-11-25 发布日期:2020-12-02
通讯作者: * 徐晓丽,E-mail: xuxiaoli@zju.edu.cn;马莲菊,E-mail: malianju@163.com
作者简介:姜媛媛(1994—),女,辽宁海城人,硕士,研究方向为转基因植物环境安全评价。E-mail: jiangyuanyuan0802@163.com

Safety evaluation of Cry-transgenic insect-resistant maize on silkworm, Bombyx mori

JIANG Yuanyuan^1,2, JI Yi^1,2, LAI Yongmin¹, CHEN Xiaoyun¹, XU Junfeng¹, XU Xiaoli^1,*, MA Lianju^2,*

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (in Prepared), Institute of Quality and Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Life Science, Shenyang Normal University, Shenyang 110034, China

Received:2020-03-24 Online:2020-11-25 Published:2020-12-02

摘要/Abstract

摘要： 家蚕可能通过取食漂移到桑叶上的玉米花粉从而受到转基因玉米中表达的Cry蛋白的影响。实验采用室内生物测试的方法评价了转cry1Ab和cry2Ab基因玉米GAB-3花粉对家蚕可能产生的影响,并采用在饲料中掺入蛋白的方式检测了转基因玉米中广泛使用的6种Cry杀虫蛋白对家蚕的毒性。当桑叶上GAB-3花粉密度为50粒·cm^-2时,对家蚕幼虫存活率无显著影响,达500粒·cm^-2时,家蚕14 d存活率为0;以10粒·cm^-2的GAB-3花粉饲喂家蚕幼虫7 d对其体重无显著不利影响,但延长饲喂时间或者当GAB-3花粉密度为50粒·cm^-2时,家蚕的体重显著低于对照组;转基因和非转基因玉米花粉处理14 d或更长时间的家蚕体重均低于无花粉处理组。与非转基因对照处理组相比,低于100粒·cm^-2的转基因玉米GAB-3花粉处理组家蚕的化蛹率、茧重、茧壳重和蛹重无显著差异,转基因玉米和非转基因玉米花粉处理均会延长家蚕幼虫的发育历期。GAB-3花粉对家蚕7 d的LC₅₀为261.18粒·cm^-2,随着处理时间延长,LC₅₀下降。测试的6种Bt杀虫蛋白对家蚕的毒性依次为Cry1C>Cry1Fa>Cry1B>Cry2A>Cry1Ab>Cry1Ac。目前,在中国接近商业化的转基因玉米中多采用的Bt杀虫蛋白为Cry1Ab和Cry2A,二者对家蚕的毒性较低,并且在实际生产中,家蚕接触到的转基因玉米花粉密度较低,因此转Cry抗虫基因玉米对家蚕的风险较低。

关键词: 转基因玉米GAB-3, Bt蛋白, 家蚕, 半致死浓度

Abstract: Larvae of the domestic silkworm, Bombyx mori(Linnaeus) could be influenced by Cry protein expressed in genetically engineered maize by consuming maize pollen. Laboratory experiments were conducted to evaluate the potential effects of transgenic maize GAB-3 expressing Cry1Ab and Cry2Ab. The toxicity of six purified Cry proteins that have been used in transgenic maize to silkworm were tested using dietary exposure assays. When the concentration of GAB-3 pollen on the leaves of mulberry was about 50 grains·cm^-2, no significant effect was detected on the survival rate of the neonate larvae of silkworm. The neonate larvae of silkworm could not survive beyond 14 d when the GAB-3 pollen concentration was about 500 grains·cm^-2. No adverse effect on the larvae weight was detected when they were fed with 10 grains·cm^-2 GAB-3 pollen for 7 days. But when the feeding time was prolonged or the density of GAB-3 pollen was more than 50 grains·cm^-2, the larvae weight was significantly decreased compared with the control larvae. The weight of the larvae decreased when they were fed with GAB-3 pollen or the non-transgenic maize pollen for 14 days or more. There was no significant difference in pupation rate, cocoon weight, pupa weight, cocoon shell weight between larvae fed with GM corn pollen or the non-transgenic corn pollen. Both of transgenic and non-transgenic maize pollen prolonged the development time of the larvae. The LC₅₀ of GAB-3 pollen to the neonate larvae of silkworm was 261.18 grains·cm^-2. While the treating time was prolonged, the LC₅₀ value decreased. Six purified Cry proteins that have been used for plant transformation were tested using dietary exposure assays. The toxicities of the tested six Cry proteins to B. mori were in the order: Cry1C>Cry1Fa>Cry1B>Cry2A>Cry1Ab>Cry1Ac. Cry1Ab and Cry2A were low toxicity to B. mori and they were commonly used in transgenic maize which was approaching commercialization in China. As in actual conditions, maize pollen density consumed by B. mori was lower than in lab, suggesting that the risk of Bt maize pollen on silkworm was negligible.

Key words: transgenic maize GAB-3, Bt protein, Bombyx mori, lethal concentration 50(LC₅₀)

中图分类号:

TS201.6

姜媛媛, 纪艺, 来勇敏, 陈笑芸, 徐俊锋, 徐晓丽, 马莲菊. 转Cry抗虫基因玉米对家蚕的安全性评价[J]. 浙江农业学报, 2020, 32(11): 2042-2049.

JIANG Yuanyuan, JI Yi, LAI Yongmin, CHEN Xiaoyun, XU Junfeng, XU Xiaoli, MA Lianju. Safety evaluation of Cry-transgenic insect-resistant maize on silkworm, Bombyx mori[J]. , 2020, 32(11): 2042-2049.

参考文献

[1] 李建琴, 顾国达, 何樟勇, 等. 中国茧丝绸产业区域布局与发展重点[J]. 蚕业科学, 2018, 44(6):936-946.
LI J Q, GU G D, HE Z Y, et al.Regional distribution and developing emphasis of China's cocoon silk industry[J]. Acta Sericologica Sinica, 2018, 44(6):936-946.(in Chinese with English abstract)
[2] 王忠华, 倪新强, 徐孟奎, 等. Bt水稻“克螟稻”花粉对家蚕生长发育的影响[J]. 遗传, 2001, 23(5):463-466.
WANG Z H, NI X Q, XU M K, et al.The effect on the development of silkworm larvae of transgenic rice pollen with a synthetic cry1Ab gene from Bacillus thuringiensis[J]. Hereditas, 2001, 23(5):463-466.(in Chinese with English abstract)
[3] YAO H W, YE G Y, JIANG C Y, et al.Effect of the pollen of transgenic rice line, TT9-3 with a fused cry1Ab/cry1Ac gene from Bacillus thuringiensis Berliner on non-target domestic silkworm, Bombyx mori Linnaeus (Lepidoptera: Bombyxidae)[J]. Applied Entomology and Zoology, 2006, 41(2):339-348.
[4] YANG Y, LIU Y, CAO F Q, et al.Consumption of Bt rice pollen containing Cry1C or Cry2A protein poses a low to negligible risk to the silkworm Bombyx mori(Lepidoptera: Bombyxidae)[J]. PLoS One, 2014, 9(7):e102302.
[5] NIU L, MA Y, MANNAKKARA A, et al.Impact of single and stacked insect-resistant bt-cotton on the honey bee and silkworm[J]. PLoS One, 2013, 8(9):e72988.
[6] 常雪. 三种转Bt基因玉米的抗虫性评估及其对非靶标经济昆虫家蚕和捕食性天敌瓢虫的安全性评价[D]. 杭州: 浙江大学, 2013.
CHANG X.Efficacy evaluation of three transgenic Bt maize events to target pests and the risk assessment to non-target silkworm and predator ladybird[D]. Hangzhou: Zhejiang University, 2013. (in Chinese with English abstract)
[7] JIAO Y Y, YANG Y, MEISSLE M, et al.Comparison of susceptibility of Chilo suppressalis and Bombyx mori to five Bacillus thuringiensis proteins[J]. Journal of Invertebrate Pathology, 2016, 136:95-99.
[8] MITSUHASHI W, MIYAMOTO K.Interaction of Bacillus thuringiensis Cry toxins and the insect midgut with a focus on the silkworm (Bombyx mori) midgut[J]. Biocontrol Science and Technology, 2020, 30(1):68-84.
[9] ENDO H, TANAKA S, ADEGAWA S, et al.Extracellular loop structures in silkworm ABCC transporters determine their specificities for Bacillus thuringiensis Cry toxins[J]. The Journal of Biological Chemistry, 2018, 293(22):8569-8577.
[10] 樊龙江, 吴月友, 庞洪泉, 等. 转基因Bt水稻花粉在桑叶上的自然飘落浓度[J]. 生态学报, 2003, 23(4):826-833.
FAN L J, WU Y Y, PANG H Q, et al.Bt rice pollen distribution on mulberry leaves near ricefileds[J]. Acta Ecologica Sinica, 2003, 23(4):826-833. (in Chinese with English abstract)
[11] HOFMANN F, OTTO M, WOSNIOK W.Maize pollen deposition in relation to distance from the nearest pollen source under common cultivation-results of 10 years of monitoring (2001 to 2010)[J]. Environmental Sciences Europe, 2014, 26(1):1-14.
[12] LANG A, OEHEN B, ROSS J H, et al.Potential exposure of butterflies in protected habitats by Bt maize cultivation: a case study in Switzerland[J]. Biological Conservation, 2015, 192:369-377.
[13] PLEASANTS J M, HELLMICH R L, DIVELY G P, et al.Corn pollen deposition on milkweeds in and near cornfields[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(21):11919-11924.
[14] YAO H W, JIANG C Y, YE G Y, et al.Toxicological assessment of pollen from different Bt rice lines on Bombyx mori(Lepidoptera: Bombyxidae)[J]. Environmental Entomology, 2008, 37(3):825-837.

转Cry抗虫基因玉米对家蚕的安全性评价

Safety evaluation of Cry-transgenic insect-resistant maize on silkworm, Bombyx mori

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

[1]	徐麒麟, 齐桂兰, 莫桂林, 朱江, 雷春龙, 吴永胜, 许祯莹, 李娟. 蚯蚓提取液对家蚕热激蛋白水平及免疫功能的影响[J]. 浙江农业学报, 2020, 32(4): 593-600.
[2]	谢道燕, 杨振国, 柴建萍, 周春涛, 罗雁婕. 8种杀虫剂对朱砂叶螨毒力及家蚕的急性毒性比较[J]. 浙江农业学报, 2018, 30(3): 420-425.
[3]	谢道燕，杨振国，周春涛，柴建萍，达爱斯，倪婧，江秀均，罗雁婕*. 新型杀菌剂健达对桑树及家蚕的安全性评价[J]. 浙江农业学报, 2016, 28(4): 654-.
[4]	夏爱华1，李娜1，贾漫丽1，毛晓霞2，李季生1，3，杨贵明1，*. 高温胁迫对家蚕血液蛋白质组的影响[J]. 浙江农业学报, 2015, 27(7): 1160-.
[5]	柳新菊;俞瑞鲜;赵学平;陈诗;蒋金花;祝新荣;*. 不同家蚕品种对毒死蜱的敏感性比较[J]. , 2014, 26(2): 0-315318.
[6]	庄俐;牛宝龙;张静静;孟智启;*. 家蚕磷脂酰肌醇3-激酶(Bmpi3k)基因的克隆及分析[J]. , 2011, 23(4): 0-691.
[7]	谢再东;李兵;王举梅;许雅香;沈卫德;*. 家蚕品种‘W-1’己₃期雌雄卵可溶性蛋白质表达谱研究[J]. , 2010, 22(3): 0-307.
[8]	王海龙;刘岩;牛宝龙;轩楠;孟智启;*. 基于AFLP的家蚕性连锁平衡致死系Z染色体连锁分子标记筛选[J]. , 2010, 22(2): 0-144.
[9]	刘培刚;李卫国;王永强;*. 家蚕蛋白质组学研究进展[J]. , 2010, 22(1): 0-129.
[10]	吴声敢;王强;赵学平;吴长兴;陈丽萍;徐浩;章虎 . 阿维菌素和氟虫腈对家蚕毒性与安全性评价研究[J]. , 2004, 16(5): 0-312.
[11]	王永强;徐孟奎;孟智启;曹锦如;姚陆松;叶爱红;何秀玲. 家蚕无性繁殖性状的基因效应分析[J]. , 2002, 14(6): 0-310.
[12]	计东风;陆旋;陈诗. 家蚕丝素蛋白对化学性肝损伤的保护作用和毒性试验[J]. , 2002, 14(6): 0-307.
[13]	陆龙喜;时连根. 家蚕血细胞对病原白僵菌的防御反应研究[J]. , 2001, 13(04): 0-204.