不同光质LED光照对草菇菌丝生长速度、菌丝分支与生物量的影响

doi:10.3969/j.issn.1004-1524.2021.06.17

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (6): 1110-1116.DOI: 10.3969/j.issn.1004-1524.2021.06.17

不同光质LED光照对草菇菌丝生长速度、菌丝分支与生物量的影响

侯立娟¹(), 李正鹏², 林金盛¹, 马林¹, 李辉平¹, 曲绍轩¹, 姜建新³, 邹秀龙³, 杨华平³, 李长田⁴^,^*(), 蒋宁¹^,^*()

1.江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,江苏南京 210014
2.上海市农业科学院食用菌研究所,上海 264025
3.江苏江南生物科技有限公司,江苏丹阳 212300
4.吉林农业大学植物保护学院,吉林长春 130018

收稿日期:2020-06-29 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 李长田,蒋宁
作者简介:蒋宁, E-mail: 24297919@qq.com
*李长田,E-mail: changtianli@126.com;
侯立娟(1981—),女,吉林松原人,博士,副研究员,主要从事食用菌的高产栽培与菌糠的综合利用研究。E-mail: mybailinggu@126.com
基金资助:
吉林农业大学食药用菌教育部工程研究中心开放课题(SYJ20119001);江苏省苏北科技专项(SZ-SQ2018039)

Effects of different light quality of LED light source on growth rate, mycelium branch and biomass of straw mushroom mycelium

HOU Lijuan¹(), LI Zhengpeng², LIN Jinsheng¹, MA Lin¹, LI Huiping¹, QU Shaoxuan¹, JIANG Jianxin³, ZOU Xiulong³, YANG Huaping³, LI Changtian⁴^,^*(), JIANG Ning¹^,^*()

1. Institute of Vegetable Crop, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China
2. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 264025, China
3. Jiangnan Biological Technology Co., Ltd., Danyang 212300, China
4. College of Plant Protection, Jilin Agricultural University, Jilin 130018, China

Received:2020-06-29 Online:2021-06-25 Published:2021-06-25
Contact: LI Changtian,JIANG Ning

摘要/Abstract

摘要：

通过探索LED光源在草菇栽培上的精准化应用,为工厂化草菇栽培提供理论依据。以草菇V23为材料,设置LED光质6个处理:对照白光(CK)、红/蓝=1∶1、红/蓝=1∶2、红/蓝=2∶1、单红光、单蓝光,研究不同光质对草菇菌丝生长的影响。结果发现,红光与蓝光的比为1∶1 菌丝长势表现为较强,对其他指标影响不明显。不同LED光源的光质对菌丝生长速度影响很大,从结果可以看出,红蓝光比例为1∶2对菌丝的生长有显著(P<0.05)的作用,并且持续保持领先有促进作用,其次是红蓝光比例为1∶1,并且LED光源的光质对菌丝的影响主要集中在前期,到菌丝后期作用不大。对草菇菌丝生物量的影响最大的是红/蓝光的比例分别为1∶2和1∶1,其分别比CK提高了28.29%和19.97%,呈极显著(P<0.01)差异;同时菌丝分支距离相对较小,分别比CK的菌丝分支距离减少了16.03%和15.13%,整体表现菌丝浓密,且长势相对较强。

关键词: LED光源, 光质, 草菇, 菌丝生长速度, 菌丝生物量, 菌丝分支

Abstract:

To explore the precise application of LED light source in the cultivation of straw mushroom and provide a theoretical basis for the cultivation of factory straw mushrooms, straw mushroom V23 was used as the material, six different treatments of LED light quality were set as: control white light (CK), red/blue=1∶1, red/blue=1∶2, red/blue=2∶1, single red light, single red light. The results showed that when the ratio of red light to blue light was 1∶1, the mycelial growth was strong, and the effect on other indexes was not obvious. The results showed that the red and blue light ratio of 1∶2 had a significant (P<0.05) effect on the growth of mycelia, and it had a promoting effect on the growth of mycelia, followed by the red and blue light ratio of 1∶1, and the effect of LED light quality on the growth of mycelia was mainly concentrated in the early stage, but not in the late stage. The ratio of red light to blue light was 1∶2 and 1∶1 showed the most effect on the mycelial biomass, which was significantly (P<0.01) higher than that of CK by 28.29% and 19.97%, respectively. At the same time, the mycelial branching distance in the treatments of red and blue light ratio of 1∶2 and 1∶1 was relatively small, and the mycelial branching distance was reduced by 16.03% and 15.13%, respectively. The mycelial growth was relatively strong and dense.

Key words: LED light, light quality, straw mushroom, mycelium growth rate, mycelium biomass, mycelium branch distance

中图分类号:

S476.1

侯立娟, 李正鹏, 林金盛, 马林, 李辉平, 曲绍轩, 姜建新, 邹秀龙, 杨华平, 李长田, 蒋宁. 不同光质LED光照对草菇菌丝生长速度、菌丝分支与生物量的影响[J]. 浙江农业学报, 2021, 33(6): 1110-1116.

HOU Lijuan, LI Zhengpeng, LIN Jinsheng, MA Lin, LI Huiping, QU Shaoxuan, JIANG Jianxin, ZOU Xiulong, YANG Huaping, LI Changtian, JIANG Ning. Effects of different light quality of LED light source on growth rate, mycelium branch and biomass of straw mushroom mycelium[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1110-1116.

图/表 6

表1 不同LED光源设计及指标

Table 1 Different LED light source indicators

灯光处理 Treatments	峰值波长 Peak wavelength/nm	半波光度 Half-wave luminosity/nm	光强 Light intensity/ (μmol·m^-2·s^-1)	红光光强 Red light intensity/ (μmol·m^-2·s^-1)	蓝光光强 Blue light intensity/ (μmol·m^-2·s^-1)
镝灯白光(CK) Control	580	—	120	—	—
红光Red light	658	± 12	120	120	—
蓝光Blue light	460	± 11	120	—	120
红光∶蓝光=1∶1	—	—	120	60	60
Red light∶Blue light=1∶1
红光∶蓝光=1∶2	—	—	120	40	80
Red light∶Blue light=1∶2
红光∶蓝光=2∶1	—	—	120	80	40
Red light∶Blue light=2∶1

表2 不同光质LED光照对草菇菌丝生长的影响

Table 2 Effect of different light quality LED light sources on mycelium growth of straw mushroom

处理 Treatment	菌落形态 Colony morphology	菌落颜色 Colony color	边缘整齐度 Edge neatmess	菌丝长势 Mycelium growth
CK Control	绒毛状Villous	白色 White	整齐 Tidy	弱 Weak
红光Red light	绒毛状Villous	白色White	整齐Tidy	弱Weak
蓝光Blue light	绒毛状Villous	白色White	整齐Tidy	弱Weak
红光∶蓝光1∶1 Red light∶Blue lighe=1∶1	绒毛状Villous	白色White	整齐Tidy	强 Strong
红光∶蓝光1∶2 Red light∶Blue lighe=1∶2	绒毛状Villous	白色White	整齐Tidy	较强 Stronger
红光∶蓝光2∶1 Red light∶Blue lighe=2∶1	绒毛状Villous	白色White	整齐Tidy	较弱 Weaker

图1 不同光质LED光照对草菇菌丝生长的影响

Fig.1 Effect of different light quality LED light sources on mycelium growth of straw mushroom

图2 不同光质LED光照对草菇菌丝生长速度的影响理时间下,不同处理间没有相同小写字母表示差异显著(P<0.05)。

Fig.2 Effect of different LED light quality on hyphae growth rate of straw mushroom Bars in the same treatment time without the same lowercase letters indicated the significant (P<0.05) difference.

图3 不同光质LED光照对草菇菌丝分支的影响不同处理间没有相同大写字母表示差异极显著(P<0.01)。下同。

Fig.3 Effect of differernt light quality LED illumination on mycelium branch of V.volvacae Bars without the same lowercase letters indicated the significant (P<0.01) difference. The same as below.

图4 不同光质LED光照对草菇菌丝生物量的影响

Fig.4 Effect of differernt light quality LED illumination on mycelium biomass of straw mushroom

参考文献 23

[1]	于海龙, 郭倩, 杨娟, 等. 环境因子对食用菌生长发育影响的研究进展[J]. 上海农业学报, 2009,25(3):100-104.
	YU H L, GUO Q, YANG J, et al. Advance in researching the effects of environmental factors on growth and development of edible fungi[J]. Acta Agriculturae Shanghai, 2009,25(3):100-104.(in Chinese with English abstract)
[2]	罗茂春, 林标声, 林跃鑫. 光质对红平菇菌丝体和子实体生长发育的影响[J]. 食品工业科技, 2012,33(8):188-190.
	LUO M C, LIN B S, LIN Y X. Effect of light quality on the growth and development of the mycelium and fruit body of Pleurotus djamor[J]. Science and Technology of Food Industry, 2012,33(8):188-190.(in Chinese with English abstract)
[3]	刘明月, 何长征, 谭金莲, 等. 光质对金针菇子实体生长发育的影响[J]. 中国食用菌, 1997,16(6):11-13.
	LIU M Y, HE C Z, TAN J L, et al. Effects of light quality on the growth and development of the fruitbody of flammulian velutipes (fr.) sing[J]. Edible Fungi of China, 1997,16(6):11-13.(in Chinese with English abstract)
[4]	王立华, 陈向东, 王秋颖, 等. LED光源的不同光质对灵芝菌丝体生长及抗氧化酶活性的影响[J]. 中国中药杂志, 2011,36(18):2471-2474.
	WANG L H, CHEN X D, WANG Q Y, et al. Effect of different light of LED light quality on growth and antioxidant enzyme activities of Ganoderma lucidum[J]. China Journal of Chinese Materia Medica, 2011,36(18):2471-2474.(in Chinese with English abstract)
[5]	刘文科, 杨其长. 食用菌光生物学及LED应用进展[J]. 科技导报, 2013,31(18):73-79.
	LIU W K, YANG Q C. Prospects in edible fungi photobiology and LED applications[J]. Science & Technology Review, 2013,31(18):73-79.(in Chinese with English abstract)
[6]	林金盛, 马林, 蒋宁, 等. 柠檬酸钠对草菇菌丝生长特性的影响[J]. 吉林农业大学学报, 2019,41(5):526-531.
	LIN J S, MA L, JIANG N, et al. Effects of sodium citrate on growth characteristics of straw mushroom mycelia[J]. Journal of Jilin Agricultural University, 2019,41(5):526-531.(in Chinese with English abstract)
[7]	MU D S, LI C Y, ZHANG X C, et al. Functions of the nicotinamide adenine dinucleotide phosphate oxidase family in Ganoderma lucidum: an essential role in ganoderic acid biosynjournal regulation, hyphal branching, fruiting body development, and oxidative-stress resistance[J]. Environmental Microbiology, 2014,16(6):1709-1728. DOI URL
[8]	侯立娟, 林金盛, 刘少华, 等. 醋酸钠及复配4种矿物质对草菇菌丝生物量的影响[J]. 浙江农业学报, 2018,30(2):228-235.
	HOU L J, LIN J S, LIU S H, et al. Sodium acetate and compound with four kinds of minerals on the mycelium biomass of Volvariella volvacea[J]. Acta Agriculturae Zhejiangensis, 2018,30(2):228-235.(in Chinese with English abstract)
[9]	SATOI S, MURAO S. Studies on pepsin inhibitor (S-PI) from streptomyces naniwaensis[J]. Agricultural and Biological Chemistry, 1971,35(10):1482-1487. DOI URL
[10]	村尾泽夫. 促进诱导子实体形成的物质[J]. 国外食用菌, 1985(3):33-36.
	MURAO SAWA. Substrances that promote the formation of fruit bodies[J]. Edible Fungi Abroad, 1985(3):33-36. (in Chinese with English abstract)
[11]	ODANI S, TOMINAGA K, KONDOU S, et al. The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinus edodes[J]. European Journal of Biochemistry, 1999,262(3):915-923. DOI URL
[12]	李玉, 于海龙, 周峰, 等. 光照对食用菌生长发育影响的研究进展[J]. 食用菌, 2011,33(2):3-4.
	LI Y, YU H L, ZHOU F, et al. Advances in researching for the effect of illumination on the growth and development of edible fungi[J]. Edible Fungi, 2011,33(2):3-4.(in Chinese)
[13]	王贺祥. 食用菌学[M]. 北京: 中国农业大学出版社, 2004.
[14]	曹哲民, 谭琦, 潘迎捷, 等. 代料花菇成因研究[J]. 食用菌学报, 2003,10(4):38-42.
	CAO Z M, TAN Q, PAN Y J, et al. A study on the formation of fissured Shiitake cultivated with artificial compost[J]. Acta Edults Fungi, 2003,10(4):38-42. (in Chinese with English abstract)
[15]	朱坚, 黄聿善. 不同颜色薄膜套袋对金针菇生长发育的影响[J]. 福建农业科技, 2000(5):14-16.
	ZHU J, HUANG Y S. The effect of different color film bagging on the growth and development of Flammulina velutipes[J]. Fujian Agricrltural Science and Technology, 2000(5):14-16. (in Chinese)
[16]	杨珊珊. 光量和光质对凤尾菇菌丝生长的影响[J]. 微生物学通报, 1984,11(4):147-148.
	YANG S S. The effect of light quantity and light quality in the growth P. edulis hyphae[J]. Microbiology , 1984,11(4):147-148.(in Chinese)
[17]	黄毅. 食用菌工厂化设施栽培的问题与对策[J]. 食用菌, 2003,25(6):2-4.
	HUANG Y. Problems and countermeasures of facility cultivation of edible fungus[J]. Edible Fungi, 2003,25(6):2-4.(in Chinese)
[18]	DAVINIA A, CARLOS A, JOSE ANTONIO A, et al. Reproducible and controllable light induction of in vitro fruiting of the white-rot basidiomycete Pleurotus ostreatus[J]. Mycological Research, 2009,113(5):552-558. DOI URL
[19]	李红, 张敏, 肖千明, 等. LED光源不同光质对金针菇、滑菇、黑木耳菌丝的影响[J]. 江苏农业科学, 2019,47(1):139-141.
	LI H, ZHANG M, XIAO Q M, et al. Effects of LED light source with different light qualities on growth of Flammulina velutipes, Pholiota nameko and Auricularia auricular-judae mycelia[J]. Jiangsu Agricultural Sciences, 2019,47(1):139-141.(in Chinese)
[20]	SONG C, CHO K, NAIR N, 等. 低温振荡处理对香菇子实体的萌发, 类脂化合物的构型和营养成分转移的影响[J]. 生物技术, 1992,2(3):46-40.
	SONG C, CHO K, NAIR N, et al. The effect of low-temperature shaking treatment on the germination of Lentinus edodes fruit body, the configuration of lipid compounds and the transfer of nutrient components[J]. Biotechnology , 1992,2(3):46-40.(in Chinese)
[21]	MOHAMED A B B, MEGURO S, KAWACHI S. The Effects of Light on primordia and fruit body formation of Lentinus edodes in a liquid medium[J]. Mokuzai Gakkaishi, 1992,38(6):600-604.
[22]	LEATHAM G F, STAHMANN M A. Effect of light and aeration on fruiting of Lentinula edodes[J]. Transactions of the British Mycological Society, 1987,88(1):9-20. DOI URL
[23]	赵春巧, 谢放, 吴萍民. 环境因素对食用菌有性发育影响的研究进展[J]. 中国农学通报, 2014,30(13):87-92.
	ZHAO C Q, XIE F, WU P M. Advance in researching the effects of environmental factors on sexual development of edible fungus[J]. Chinese Agricultural Science Bulletin, 2014,30(13):87-92.(in Chinese with English abstract)

不同光质LED光照对草菇菌丝生长速度、菌丝分支与生物量的影响

Effects of different light quality of LED light source on growth rate, mycelium branch and biomass of straw mushroom mycelium

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 23

相关文章 10

编辑推荐

Metrics

本文评价

[1]	许建民, 史和娣, 史培华, 张泽洋, 徐志刚. 不同光质条件下马铃薯光响应曲线拟合模型的比较[J]. 浙江农业学报, 2020, 32(5): 753-761.
[2]	侯立娟, 林金盛, 刘少华, 李瑞祥, 马林, 蒋宁, 曲绍轩, 李辉平. 醋酸钠及复配4种矿物质对草菇菌丝生物量的影响[J]. 浙江农业学报, 2018, 30(2): 228-235.
[3]	张晓梅, 胡超轶, 刘涛, 周艳虹. 不同光质对黄瓜幼苗抗旱性的影响[J]. 浙江农业学报, 2017, 29(1): 58-63.
[4]	李慧敏，陆晓民，赵诗浩. 不同LED光质对秋葵幼苗生长、生理特性和气孔特征的影响[J]. 浙江农业学报, 2016, 28(6): 966-.
[5]	张云婷1，宋霞1，叶云天1，冯琛1，孙勃1，王小蓉2，汤浩茹1，*. 光质对低温胁迫下草莓叶片生理生化特性的影响[J]. 浙江农业学报, 2016, 28(5): 790-.
[6]	王兴翠1，2. 光质对试管生姜生长发育及微型姜形成、品质的影响[J]. 浙江农业学报, 2015, 27(7): 1178-.
[7]	韦峰，祁娟霞，李佳梅，韩泽宇，刘卫成，张亚红*. 不同光质对辣椒种子萌发、幼苗生长及抗寒性的影响[J]. 浙江农业学报, 2015, 27(11): 1932-.
[8]	孙洪助;王虹;沈建华;郭世荣;朱为民;*;许爽. 不同比例红蓝光对生菜种子萌发及幼苗生长的影响[J]. , 2014, 26(3): 0-603608.
[9]	孙庆丽;陈志;徐刚;陈敏;陈剑平;*. 不同光质对水稻幼苗生长的影响[J]. , 2010, 22(3): 0-325.
[10]	赵淼;林毅;蔡永萍;谢鸣;蒋桂华;吴延军. 不同光质对草莓果实成熟过程中色素类物质含量的影响[J]. , 2008, 20(1): 0-66.