饲粮添加复合益生菌对产蛋后期蛋鸡生产性能、肠道抗氧化能力和肠道氨氮含量的影响

doi:10.3969/j.issn.1004-1524.2023.02.04

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (2): 275-284.DOI: 10.3969/j.issn.1004-1524.2023.02.04

饲粮添加复合益生菌对产蛋后期蛋鸡生产性能、肠道抗氧化能力和肠道氨氮含量的影响

陈欣¹(), 慈文佳¹, 李浙烽², 蔡平梨³, 朱建津¹^,^*()

1.江南大学食品学院,江苏无锡 214122
2.杭州康德权饲料有限公司,浙江杭州 311107
3.徐州市铜山区家禽技术指导站,江苏徐州 221100

收稿日期:2021-11-19 出版日期:2023-02-25 发布日期:2023-03-14
通讯作者: 朱建津
作者简介:*朱建津,E-mail: 13706191551@163.com
陈欣(1996—),男,四川宜宾人,硕士研究生,研究方向为营养与功能因子。E-mail:xin092695@163.com
基金资助:
国家自然科学基金(31901679);江苏省自然科学基金(20190594)

Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens

CHEN Xin¹(), CI Wenjia¹, LI Zhefeng², CAI Pingli³, ZHU Jianjin¹^,^*()

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2. Hangzhou King Technology Feed Co., Ltd., Hangzhou 311107, China
3. Poultry Technical Guidance Station, Tongshan District, Xuzhou, Xuzhou 221100, Jiangsu, China

Received:2021-11-19 Online:2023-02-25 Published:2023-03-14
Contact: ZHU Jianjin

摘要/Abstract

摘要：

为研究饲粮添加嗜酸乳杆菌和枯草芽孢杆菌复合制剂对产蛋后期生产性能和肠道健康的影响,选取2 160只50周龄的海兰褐蛋鸡随机分为3组,分别饲喂基础日粮(NC组)以及添加250 mg·kg^-1(LD组)或500 mg·kg^-1(HD组)嗜酸乳杆菌和枯草芽孢杆菌复合制剂的日粮。结果表明:与NC组相比,LD和HD组蛋鸡产蛋率在49 d时分别提高了2.62%和2.20%,HD组蛋鸡平均日采食量(ADFI)显著降低(P<0.05)。与NC组相比,LD组的回肠组织中过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和盲肠总抗氧化能力(T-AOC)显著升高(P<0.05);HD组中盲肠和回肠的T-AOC、GSH-Px活性,CAT活性以及盲肠组织中超氧化物歧化酶(SOD)活性显著增加(P<0.05)。LD组盲肠内容物中乙酸、正戊酸和异戊酸含量显著上升(P<0.05),HD组中乙酸、丙酸和正丁酸含量显著升高(P<0.05);HD组盲肠内容物中氨氮、尿素含量和尿酸酶相对活性显著降低(P<0.05),尿酸水平升高(P<0.05)。盲肠微生物和氨代谢物相关性分析表明,拟杆菌、梭菌、瘤胃球菌等微生物与氨代谢产物呈显著相关性。综上所述,饲粮添加嗜酸乳杆菌和枯草芽孢杆菌复合制剂可以提高肠道抗氧化能力,增加盲肠内容物中短链脂肪酸水平,减少肠道内容物中氨氮含量,从而提高产蛋后期蛋鸡的生产性能。本研究有助于阐明益生菌复合制剂对产蛋后期蛋鸡生产性能的影响,为提高蛋鸡生产性能提供潜在的干预手段。

关键词: 益生菌, 产蛋后期蛋鸡, 生产性能, 肠道健康

Abstract:

This study was aimed to investigate the effects of of dietary supplementation of Lactobacillus acidophilus and Bacillus subtilis on the performance and intestinal health of late-phase laying hens. A total of 2 160 Hy-Line brown hens (50-weeks-old) were randomly divided into three groups, the control group (NC group) was fed the basal diet and the experimental group was supplemented with 250 mg·kg^-1 (LD group) and 500 mg·kg^-1 (HD group) Lactobacillus acidophilus and Bacillus subtilis complexes on the basal diet. The results showed that compared with the NC group, the egg production rate increased by 2.62% and 2.20% in the LD and HD groups respectively and the average daily feed intake (ADFI) was significantly lower in the HD group (P<0.05) at the 49 d. Compared with the NC group, the activity of catalase (CAT) and glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in ileal tissue in the LD group were significantly higher than those in the NC group. Similarly, T-AOC, GSH-Px, CAT and SOD activities in cecum and ileum tissues were significantly increased (P<0.05) in the HD group. Significantly higher levels of acetic acid, n-valeric acid and isovaleric acid in cecum contents in the LD group (P<0.05), and significantly higher levels of acetic acid, propionic acid and n-butyric acid in the HD group (P<0.05) compared with the NC group. Ammonia nitrogen, urea content and relative activity of urease in the cecum contents were significantly lower (P<0.05) compared with the NC group. However, the levels of uric acid were higher (P<0.05) in the HD group compared with the NC group. The correlation between microbiome and ammonia metabolites showed that the relative abundance (Bacteroides, Clostridium, Ruminococcus, etc.) were significantly correlated with ammonia metabolites. In conclusion, dietary supplementation of Lactobacillus acidophilus and Bacillus subtilis can improve the antioxidant capacity of the intestine, increase the level of short-chain fatty acids in the intestinal contents and reduce the ammonia content in the intestinal contents for improving the performance of laying hens in the late egg-laying period.

Key words: probiotics, laying hen, production performance, gut health

中图分类号:

S831

陈欣, 慈文佳, 李浙烽, 蔡平梨, 朱建津. 饲粮添加复合益生菌对产蛋后期蛋鸡生产性能、肠道抗氧化能力和肠道氨氮含量的影响[J]. 浙江农业学报, 2023, 35(2): 275-284.

CHEN Xin, CI Wenjia, LI Zhefeng, CAI Pingli, ZHU Jianjin. Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 275-284.

图/表 9

表1 基础日粮营养组成与含量

Table 1 Composition and nutrient levels of basal diet

原料组成Crude material	含量Content/(g·kg^-1)	营养水平Nutritional level	总计Total
玉米Corn grain	620.00	代谢能Metabolic energy/(MJ·kg^-1)	11.02
豆粕Soybean meal	250.00	粗蛋白Crude protein/%	16.85
磷酸氢钙Dicalcium phosphate	40.00	钙Calcium/%	3.20
石粉Limestone	80.00	有效磷Available phosphorus/%	0.35
预混料Premix¹⁾	10.00	赖氨酸Lysine/%	0.85
合计Total	1 000.00	蛋氨酸+胱氨酸Methionine+Cystine/%	0.63

图1 饲粮添加复合益生菌对产蛋后期蛋鸡生产性能的影响 *代表NC组与LD组间有显著差异(P<0.05),#代表HD组与NC组间有显著差异(P<0.05);柱上无相同字母的表示组间差异显著(P<0.05)。下同。

Fig.1 Effects of dietary supplementation with compound probiotics on laying performance of late-phase laying hens *represented a significant difference between NC group and LD group (P<0.05), and # represented a significant difference between HD group and NC group (P<0.05); No same letters on the bars indicated significant difference among groups (P<0.05). The same as below.

表2 饲粮添加复合益生菌对产蛋后期蛋鸡蛋品质的影响

Table 2 Effects of dietary supplementation with compound probiotics on egg quality of late-phase laying hens

指标Parameters	NC	LD	HD
蛋壳强度	41.89±8.64	40.97±5.25	42.12±9.50
Eggshell strength/N
蛋白高度	5.87±1.51	5.96 ±1.59	6.72 ±0.69
Albumen height/mm
蛋黄颜色	6.97±1.05	6.99±0.70	6.63±0.32
Yolk color
哈夫单位	73.36±13.32	72.04±12.78	78.86±7.01
Haugh units
蛋形指数	1.30±0.04	1.32±0.04	1.31±0.05
Egg shape index

图2 饲粮添加复合益生菌对产蛋后期蛋鸡盲肠和回肠组织抗氧化能力的影响

Fig.2 Effects of dietary supplementation with compound probiotics on the antioxidant capacity of cecum and ileum tissues in late-phase laying hens

图3 饲粮添加复合益生菌对产蛋后期蛋鸡盲肠组织形态结果的影响(H&E染色,×200)

Fig.3 Effects of dietary supplementation with compound probiotics on the histopathology of cecum in late-phase laying hens (H&E staining, ×200)

图4 饲粮添加复合益生菌对产蛋后期蛋鸡盲肠内容物和血浆中氨代谢物含量的影响

Fig.4 Effects of dietary supplementation with compound probiotics on the ammonia-related metabolites in the contents of the cecum and plasma in late-phase laying hens

表3 饲粮补充复合益生菌对产蛋后期蛋鸡盲肠内容物中氨代谢相关酶活性的影响

Table 3 Effects of dietary supplementation with compound probiotics on the ammonia-related enzyme activity in the contents of the cecum in late-phase laying hens

项目Item	NC	LD	HD
脲酶活性 Urease activity/(U·mg^-1)	16.42 ±1.81	16.09 ±3.4	10.11 ±1.95
尿酸酶相对活性 The activity of uricase/%	100.42 ±2.04 a	91.83 ±1.52 ab	88.67 ±3.51 b
谷氨酸脱氢酶活性 GDH activity/(U·mg^-1)	31.65 ±3.37	39.81 ±5.64	35.19 ±3.16
谷氨酰胺合成酶活性 GS activity/(μmol·h^-1·	0.96 ±0.09	1.09 ±0.14	1.18 ±0.09
mg^-1 prot)

图5 饲粮补充嗜酸乳杆菌和枯草芽孢杆菌复合制剂对产蛋后期蛋鸡盲肠内容物中短链脂肪酸含量的影响

Fig.5 Effects of dietary supplementation with compound probiotics on the short-chain fatty acids in contents of cecum in late-phase laying hens

图6 产蛋后期蛋鸡盲肠内容物中氨氮、尿素、尿酸与盲肠微生物相对丰度相关性分析

Fig.6 Correlation between ammonia nitrogen, urea and uric acid in cecum contents and relative abundance of cecum microbial in late-phase laying hens

参考文献 37

[1]	王伟唯. 丁酸梭菌对产蛋后期蛋鸡肠道功能和脂质代谢的调节作用[D]. 北京: 中国农业科学院, 2020.
	WANG W W. Regulatory effects of Clostridium butyricum on intestinal functions and lipid metabolism of laying hens in the late phase of production[D]. Beijing: Chinese Academy of Agricultural Sciences, 2020. (in Chinese with English abstract)
[2]	云环, 崔凤云, 孔维恒, 等. 我国蛋鸡养殖中药物残留分析研究及监管建议[J]. 食品安全质量检测学报, 2021, 12(12): 5072-5078.
	YUN H, CUI F Y, KONG W H, et al. Analysis and supervision suggestion on drug residue in laying hens cultivation[J]. Journal of Food Safety & Quality, 2021, 12(12): 5072-5078. (in Chinese with English abstract)
[3]	HOU C L, ZENG X F, YANG F J, et al. Study and use of the probiotic Lactobacillus reuteri in pigs: a review[J]. Journal of Animal Science and Biotechnology, 2015, 6(1): 14.
[4]	OH J K, PAJARILLO E A B, CHAE J P, et al. Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum[J]. Journal of Animal Science and Biotechnology, 2017, 8: 1.
[5]	XIE S, ZHAO S Y, JIANG L, et al. Lactobacillus reuteri stimulates intestinal epithelial proliferation and induces differentiation into goblet cells in young chickens[J]. Journal of Agricultural and Food Chemistry, 2019, 67(49): 13758-13766.
[6]	FORTE C, MANUALI E, ABBATE Y, et al. Dietary Lactobacillus acidophilus positively influences growth performance, gut morphology, and gut microbiology in rurally reared chickens[J]. Poultry Science, 2018, 97(3): 930-936.
[7]	WANG W W, WANG J, ZHANG H J, et al. Effects of Clostridium butyricum on production performance and intestinal absorption function of laying hens in the late phase of production[J]. Animal Feed Science and Technology, 2020, 264: 114476.
[8]	蔡访勤. 肠道正常菌群与人体健康和疾病密切相关[J]. 河南医学研究, 2001, 10(2): 171-174.
	CAI F Q. The normal intestinal bacterial flora have relation to the health and disease of host[J]. Henan Medical Research, 2001, 10(2): 171-174. (in Chinese)
[9]	张俊. 益生菌混合发酵饲料及其产物对肉鸡肠道健康的影响[D]. 无锡: 江南大学, 2016.
	ZHANG J. Effects of fermented feed and its fermentation metabolite produced by multiple probiotics on intestinal health of chicken[D]. Wuxi: Jiangnan University, 2016. (in Chinese with English abstract)
[10]	VISEK W J. Ammonia: its effects on biological systems, metabolic hormones, and reproduction[J]. Journal of Dairy Science, 1984, 67(3): 481-498.
[11]	WEI F X, HU X F, SA R N, et al. Antioxidant capacity and meat quality of broilers exposed to different ambient humidity and ammonia concentrations[J]. Genetics and Molecular Research: GMR, 2014, 13(2): 3117-3127.
[12]	NICAISE C, PROZZI D, VIAENE E, et al. Control of acute, chronic, and constitutive hyperammonemia by wild-type and genetically engineered Lactobacillus plantarum in rodents[J]. Hepatology, 2008, 48(4): 1184-1192.
[13]	YEO J, KIM K. Effect of feeding diets containing an antibiotic, a probiotic, or Yucca extract on growth and intestinal urease activity in broiler chicks[J]. Poultry Science, 1997, 76(2): 381-385.
[14]	GUPTA M, PATTANAIK A K, SINGH A, et al. An appraisal of the gut health modulatory effects of a calf faecal-origin probiotic Lactobacillus salivarius CPN60 using Wistar rats with dextran sulfate sodium-induced colitis[J]. Journal of the Science of Food and Agriculture, 2021, 101(4): 1340-1348.
[15]	CHAPMAN C M C, GIBSON G R, ROWLAND I. Health benefits of probiotics: are mixtures more effective than single strains?[J]. European Journal of Nutrition, 2011, 50(1): 1-17.
[16]	LIU X, XIA B, HE T, et al. Oral administration of a select mixture of Lactobacillus and Bacillus alleviates inflammation and maintains mucosal barrier integrity in the ileum of pigs challenged with Salmonella infantis[J]. Microorganisms, 2019, 7(5): 135.
[17]	CUTTING S M. Bacillus probiotics[J]. Food Microbiology, 2011, 28(2): 214-220.
[18]	杨欣, 杨小军, 李绍钰, 等. 我国白羽肉鸡一般饲料添加剂研究进展[J]. 动物营养学报, 2020, 32(10): 4592-4601.
	YANG X, YANG X J, LI S Y, et al. Research advances in common feed additives of white feather broilers in China[J]. Chinese Journal of Animal Nutrition, 2020, 32(10): 4592-4601. (in Chinese with English abstract)
[19]	FENG J, LU M Y, WANG J, et al. Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in late-phase laying hens[J]. Journal of Animal Science and Biotechnology, 2021, 12(1): 72.
[20]	刘江英, 朱建津, 蒋蓉, 等. 多菌种联合发酵饲料对肉鸡抗沙门氏菌感染能力的影响[J]. 浙江农业学报, 2019, 31(2): 229-234.
	LIU J Y, ZHU J J, JIANG R, et al. Effect of multi-strain co-fermented feed on anti-Salmonella infection in broilers[J]. Acta Agriculturae Zhejiangensis, 2019, 31(2): 229-234. (in Chinese with English abstract)
[21]	钟智康. 盲肠微生物调控蛋鸡氨气产生的丁酸钠干预机制[D]. 广州: 华南农业大学, 2018.
	ZHONG Z K. Mechanism of cecal microflora regulates the ammonia production from laying hens in the intervention of sodium butyrate[D]. Guangzhou: South China Agricultural University, 2018. (in Chinese with English abstract)
[22]	姚泽晨. 植物乳杆菌微胶囊及其壁材对小鼠肥胖的预防作用[D]. 无锡: 江南大学, 2019.
	YAO Z C. The preventative effect of Lactobacillus plantarum microcapsule and its wall material on diet induced obesity in mice[D]. Wuxi: Jiangnan University, 2019. (in Chinese with English abstract)
[23]	LIU Y, LUO Y K, WANG X H, et al. Gut microbiome and metabolome response of Pu-erh tea on metabolism disorder induced by chronic alcohol consumption[J]. Journal of Agricultural and Food Chemistry, 2020, 68(24): 6615-6627.
[24]	朱沛霁. 枯草芽孢杆菌对雪山鸡生产性能、肠道健康和免疫机能的影响及机制[D]. 扬州: 扬州大学, 2017.
	ZHU P J. Effects of dietary supplementation of Bacillus subtilis on growth performance, intestinal health and immune functional responses of Xueshan broiler[D]. Yangzhou: Yangzhou University, 2017. (in Chinese with English abstract)
[25]	GUO J R, DONG X F, LIU S, et al. Effects of long-term Bacillus subtilis CGMCC 1.921 supplementation on performance, egg quality, and fecal and cecal microbiota of laying hens[J]. Poultry Science, 2017, 96(5): 1280-1289.
[26]	MOSER H. Electrophysiological evidence for ammonium as a substitute for potassium in activating the sodium pump in a crayfish sensory neuron[J]. Canadian Journal of Physiology and Pharmacology, 1987, 65(2): 141-145.
[27]	DASARATHY S, MOOKERJEE R P, RACKAYOVA V, et al. Ammonia toxicity: from head to toe?[J]. Metabolic Brain Disease, 2017, 32(2): 529-538.
[28]	WEI F X, XU B, HU X F, et al. The effect of ammonia and humidity in poultry houses on intestinal morphology and function of broilers[J]. Journal of Animal and Veterinary Advances, 2012, 11(19): 3641-3646.
[29]	SHEN T C D, ALBENBERG L, BITTINGER K, et al. Engineering the gut microbiota to treat hyperammonemia[J]. The Journal of Clinical Investigation, 2015, 125(7): 2841-2850.
[30]	LIU J, LKHAGVA E, CHUNG H J, et al. The pharmabiotic approach to treat hyperammonemia[J]. Nutrients, 2018, 10(2): 140.
[31]	谢俊华. 植物乳杆菌NCU116对肠道健康的影响[D]. 南昌: 南昌大学, 2016.
	XIE J H. The effects of Lactobacillus plantarum NCU116 on intestinal health[D]. Nanchang: Nanchang University, 2016. (in Chinese with English abstract)
[32]	李俊婷. 樟属植物提取工艺对鸡排泄物中尿酸酶及其产生菌的影响[D]. 杭州: 浙江大学, 2004.
	LI J T. Effects of cinnamon extraction methods on uricase and uricase-generating microbs in poultry excretion[D]. Hangzhou: Zhejiang University, 2004. (in Chinese with English abstract)
[33]	张阳, 吕慧源, 徐盛玉, 等. 复合益生菌与黄芪多糖对生长育肥猪生长性能、血清生化指标和粪便微生物的影响[J]. 动物营养学报, 2021, 33(6): 3542-3553.
	ZHANG Y, LYU H Y, XU S Y, et al. Effects of compound probiotics and Astragalus polysaccharide on growth performance, serum biochemical indices and fecal microorganism of growing-finishing pigs[J]. Chinese Journal of Animal Nutrition, 2021, 33(6): 3542-3553. (in Chinese with English abstract)
[34]	LI J M, YU R, ZHANG L P, et al. Dietary fructose-induced gut dysbiosis promotes mouse hippocampal neuroinflammation: a benefit of short-chain fatty acids[J]. Microbiome, 2019, 7(1): 98.
[35]	SUN M M, WU W, CHEN L, et al. Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis[J]. Nature Communications, 2018, 9(1): 3555.
[36]	CUMMINGS J H, MACFARLANE G T. The control and consequences of bacterial fermentation in the human colon[J]. Journal of Applied Bacteriology, 1991, 70(6): 443-459.
[37]	SHEN T C D, FRIEDMAN E S, HERMAN L L, et al. Sa1929 bacterial urease modulates both microbe-microbe and microbe-host interactions through nitrogen metabolism[J]. Gastroenterology, 2020, 158(6): S-484.

编辑推荐

Metrics

阅读次数

全文

437

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	8	0	0	429

来源	本网站	其他网站

次数	242	195
比例	55%	45%

摘要

256

最新录用	在线预览	正式出版

0	0	256

	来源	本网站

	次数	256
	比例	100%

饲粮添加复合益生菌对产蛋后期蛋鸡生产性能、肠道抗氧化能力和肠道氨氮含量的影响

Effects of dietary supplementation with compound probiotics on production performance, intestinal antioxidant capacity and intestinal ammonia nitrogen levels in late-phase laying hens

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 37

相关文章 12

编辑推荐

Metrics

本文评价

[1]	刘翠艳, 陈四玉, 孙桃桃, 尚莉莉, 杨明川, 杨孜生, 韩春杨. 马齿苋-益生菌联用对热毒证小鼠腹泻的预防效果[J]. 浙江农业学报, 2018, 30(2): 211-219.
[2]	张李荣, 杨海明, 龚道清, 肖霞, 唐徐华, 王志跃. 应用Illumina MiSeq高通量测序技术分析饲粮添加乳双歧杆菌对蛋雏鸡盲肠微生物的影响[J]. 浙江农业学报, 2018, 30(10): 1630-1639.
[3]	赵青. 猪圆环病毒疫苗对仔猪生产性能的效果评估[J]. 浙江农业学报, 2018, 30(10): 1662-1664.
[4]	徐毓琴1，戴茜茜1，唐慧琴1，谷笑笑1，潘康成1,2,*. 产γ氨基丁酸益生菌的筛选及其生物活性研究[J]. 浙江农业学报, 2016, 28(3): 502-.
[5]	张变英，贺东昌*，王芳，张红岗，张元庆，上官明军，武霞，樊爱芳. 日粮添加维生素D3对肉鸡生产性能、屠宰性能、免疫功能和肌肉品质的影响[J]. 浙江农业学报, 2015, 27(8): 1350-.
[6]	赵燕，吴春琴，孙思维，金俊杰*. 辐照豆粕对肉鸡生产性能、免疫及抗氧化功能的影响[J]. 浙江农业学报, 2015, 27(6): 939-.
[7]	乔富强;乔利敏;姚华;*. 不同添加剂对蛋鸡生产性能和蛋品质的影响[J]. , 2013, 25(6): 0-1219.
[8]	张豫超;杨肖芳;苗立祥;蒋桂华*. 三种草莓立体栽培架型及生产性能比较 [J]. , 2013, 25(6): 0-1292.
[9]	乔富强;乔利敏;姚华;*. 复合生物预混剂对奶牛生产性能和乳品质的影响[J]. , 2013, 25(4): 0-713.
[10]	张永静;徐维娜;刘文斌*;李贵锋;夏薇;邵仙萍 . 饲料铅对建鲤生产性能及组织酶活性的影响[J]. , 2012, 24(2): 0-227.
[11]	卢福庄;王志刚;付媛;石团员;华卫东;张雪娟. 中草药添加剂对猪生产性能和猪瘟疫苗免疫效果的影响[J]. , 2011, 23(1): 56-61.
[12]	程菊芬;刘建新;蒋永清;*;徐海风;徐美英. 木瓜粉对新西兰白兔生产性能和免疫功能的影响[J]. , 2009, 21(05): 0-462.