浙江农业学报 ›› 2023, Vol. 35 ›› Issue (2): 425-433.DOI: 10.3969/j.issn.1004-1524.2023.02.20

• 食品科学 • 上一篇    下一篇

OSA改性多孔淀粉负载并改善柚皮苷生物利用度

王璐1(), 丁喆1,2, 陆胜民1,*(), 姜昊1,3, 郑美瑜1, 杨颖1   

  1. 1.浙江省农业科学院 食品科学研究所,浙江 杭州 310021
    2.浙江工业大学 食品科学与工程学院, 浙江 杭州 310014
    3.奥克兰大学 化学科学学院, 奥克兰1010
  • 收稿日期:2022-04-20 出版日期:2023-02-25 发布日期:2023-03-14
  • 通讯作者: 陆胜民
  • 作者简介:*陆胜民,E-mail: lushengmin@hotmail.com
    王璐(1989—),女,黑龙江哈尔滨人,博士,助理研究员,主要从事药用植物活性成分研究与开发。E-mail: wanglu1286@163.com
  • 基金资助:
    国家自然科学基金(31571892)

OSA-modified porous starch loading and improving bioavailability of naringin

WANG Lu1(), DING Zhe1,2, LU Shengmin1,*(), JIANG Hao1,3, ZHENG Meiyu1, YANG Ying1   

  1. 1. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    2. College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China
    3. School of Chemical Sciences, Auckland University, Auckland 1010, New Zealand
  • Received:2022-04-20 Online:2023-02-25 Published:2023-03-14
  • Contact: LU Shengmin

摘要:

为通过优化实验提升柚皮苷(NA)OSA改性多孔淀粉(OSAPS)中的负载率,并进而以复合物的形式提升NA在大鼠体内的相对生物利用度,通过反溶剂沉淀法制备了OSAPS-NA复合物,并对最佳条件下制备的复合物进行代谢动力学研究。通过隔室模型和统计矩模型的拟合对NA原材料和OSAPS-NA复合物的口服生物利用度进行评价。结果表明:采用丙酮为溶剂时,溶剂共沉淀结合超声法能够明显提升NA在OSAPS中的负载率,并且当正己烷的体积占比为0.6、超声强度为200 W、超声时间为20 min时,OSAPS对NA的负载率最高。口服相对生物利用度结果表明,最佳条件下制备的OSAPS-NA复合物中NA的药-时曲线下面积是NA原材料的9.80倍,达峰时间由60 min缩短至15 min。该研究成功优化了OSAPS-NA复合物,使其对NA的负载率达到了(71.49±2.26)%,并且通过OSAPS的负载使NA的口服相对生物利用度提升到原来的9.80倍。

关键词: OSA改性淀粉, 多孔淀粉, 柚皮苷, 反溶剂沉淀法, 口服生物利用度

Abstract:

In order to enhance the loading of naringin (NA) in OSA modified porous starch (OSAPS) by optimization experiments and further enhance the in vivo bioavailability of NA with the form of complex, OSAPS-NA complexes were prepared by antisolvent precipitation method and metabolic kinetic studies were performed on the complexes prepared under optimal conditions. The oral bioavailability of the raw NA material and the OSAPS-NA complex was evaluated by fitting the compartmental model and the statistical moment model. The result showed that when acetone was applied as the solvent, solvent co-precipitation combined with ultrasonication significantly enhanced the loading rate of NA in OSAPS, and the highest loading rate of NA in OSAPS was achieved when the volume ratio of antisolvent (n-hexane) was 0.6, the sonication time was 200 W and the sonication time was 20 min. The oral relative bioavailability results indicated that the area under the drug-time curve of NA in the OSAPS-NA complex prepared under optimal conditions was 9.80 times that of the original NA material, and the time to peak was reduced from 60 min to 15 min. The study successfully optimized the OSAPS-NA complex, resulting in approximately (71.49±2.26)% loading of NA. And by being loaded by OSAPS, the relative oral bioavailability of NA was increased by 9.80 times.

Key words: OSA modified starch, porous starch, naringin, antisolvent precipitation method, oral bioavailability

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