鹌鹑GnRH-1基因多态性与蛋品质的关联分析

doi:10.3969/j.issn.1004-1524.2023.03.09

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (3): 565-574.DOI: 10.3969/j.issn.1004-1524.2023.03.09

鹌鹑GnRH-1基因多态性与蛋品质的关联分析

王龙威¹(), 白俊艳¹^,²^,^*(), 贾小平³, 雷莹¹^,², 陈梦柯¹, 樊红灯¹, 卢小宁¹, 何豫涵¹, 曾凡林¹, 张容恺¹

1.河南科技大学动物科技学院,河南洛阳 471023
2.洛阳市动物遗传育种重点实验室,河南洛阳 471023
3.河南科技大学农学院(牡丹学院),河南洛阳 471023

收稿日期:2022-05-07 出版日期:2023-03-25 发布日期:2023-04-07
通讯作者: ^*白俊艳,E-mail:junyanbai@163.com
作者简介:王龙威(1998—),男,河南周口人,硕士研究生,主要从事动物分子遗传育种研究。E-mail:1575880518@qq.com

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

WANG Longwei¹(), BAI Junyan¹^,²^,^*(), JIA Xiaoping³, LEI Ying¹^,², CHEN Mengke¹, FAN Hongdeng¹, LU Xiaoning¹, HE Yuhan¹, ZENG Fanlin¹, ZHANG Rongkai¹

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China
2. Luoyang Key Laboratory of Animal Genetics and Breeding, Luoyang 471023, Henan, China
3. College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2022-05-07 Online:2023-03-25 Published:2023-04-07

摘要/Abstract

摘要：

为探索促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)基因亚型GnRH-1基因与鹌鹑蛋品质的相关性,利用PCR扩增和DNA测序技术,检测GnRH-1基因在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑中的单核苷酸多态性(SNP)突变位点,并分析GnRH-1基因的多态性与鹌鹑蛋品质的关联性,以期获得鹌鹑蛋品质相关的分子标记,为蛋用鹌鹑新品系的培育提供参考。结果表明,GnRH-1基因在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑中检测到14个SNP突变位点,分别为C71T、C108T、C168T、C178T、A184G、C206T、A209C、C215T、A252G、A279T、C281T、C293G、C339T、C458T,除A209C和C281T在中国黄羽鹌鹑和北京白羽鹌鹑中只检测到2种基因型外,其他12个SNP位点在3个鹌鹑品种中均检测到3种基因型。关联分析表明,GnRH-1基因的C71T、C215T、A209C、A279G位点与鹌鹑的蛋黄高度和蛋黄指数有显著(P<0.05)相关性,A209C位点与鹌鹑的蛋黄宽度有显著(P<0.05)相关性,A279G位点与鹌鹑的蛋壳厚度有显著(P<0.05)相关性。综上所述,GnRH-1基因可以作为影响鹌鹑蛋品质的分子标记或候选基因。

关键词: 鹌鹑, GnRH-1基因, 多态性, 蛋品质, 蛋黄

Abstract:

To explore the correlation between GnRH-1 gene of gonadotropin releasing hormone (GnRH) gene subtype and quail egg quality, the single nucleotide polymorphism (SNP) mutation site of GnRH-1 gene in Chinese yellow quails, Beijing white quails and Korean quails was detected by PCR amplification and DNA sequencing, the polymorphism of GnRH-1 gene and its association with quality of quail eggs were analyzed in order to obtain the molecular markers related to the quality of quail eggs. The results showed that 14 SNP mutations of GnRH-1 gene were detected in Chinese yellow quails, Beijing white quails and Korean quails, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T, C458T. Only two genotypes of A209C and C281T were detected in Chinese yellow quail and Beijing white quail, while other 12 SNP loci detected three genotypes in three quail varieties. Correlation analysis showed that there were significant (P<0.05) correlations between C71T, C215T, A209C and A279G loci of GnRH-1 gene and yolk height and yolk index, and between the A209C locus and the yolk width. There was a significant (P<0.05) correlation between A279G locus and eggshell thickness of quail. In conclusion, GnRH-1 gene could be used as a molecular marker or candidate gene for quail egg quality.

Key words: quail, GnRH-1gene, polymorphism, egg quality, yolk

中图分类号:

S839

王龙威, 白俊艳, 贾小平, 雷莹, 陈梦柯, 樊红灯, 卢小宁, 何豫涵, 曾凡林, 张容恺. 鹌鹑GnRH-1基因多态性与蛋品质的关联分析[J]. 浙江农业学报, 2023, 35(3): 565-574.

WANG Longwei, BAI Junyan, JIA Xiaoping, LEI Ying, CHEN Mengke, FAN Hongdeng, LU Xiaoning, HE Yuhan, ZENG Fanlin, ZHANG Rongkai. Association analysis between GnRH-1 gene polymorphism and egg quality in quail[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 565-574.

图/表 5

参考文献 23

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位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	基因型 Genotype	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	CC	0.104	0.159	0.042	C215T	CC	0.354	0.659	0.250
	CT	0.417	0.318	0.313		CT	0.438	0.295	0.500
	TT	0.479	0.522	0.534		TT	0.208	0.046	0.250
C108T	CC	0.458	0.500	0.438	A252G	AA	0.104	0.205	0.125
	CT	0.417	0.319	0.417		AG	0.438	0.318	0.458
	TT	0.125	0.181	0.146		GG	0.458	0.477	0.417
C168T	CC	0.125	0.182	0.125	A279T	AA	0.085	0.100	0.109
	CT	0.417	0.341	0.479		AT	0.809	0.800	0.804
	TT	0.458	0.477	0.396		TT	0.106	0.100	0.087
C178T	CC	0.458	0.477	0.396	C281T	CC	0	0	0.063
	CT	0.417	0.341	0.438		CT	0.020	0.045	0.229
	TT	0.125	0.182	0.167		TT	0.980	0.955	0.708
A184G	AA	0.458	0.477	0.375	C293G	CC	0.104	0.159	0.042
	AG	0.417	0.341	0.458		CG	0.438	0.318	0.312
	GG	0.125	0.182	0.167		GG	0.458	0.523	0.646
C206T	CC	0.125	0.182	0.187	C339T	CC	0.833	0.795	0.688
	CT	0.438	0.364	0.542		CT	0.146	0.182	0.186
	TT	0.437	0.454	0.291		TT	0.021	0.021	0.125
A209C	AA	0	0	0.063	C458T	CC	0.563	0.341	0.604
	AC	0.020	0.045	0.313		CT	0.396	0.386	0.313
	CC	0.980	0.955	0.624		TT	0.041	0.273	0.083

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail	位点 Locus	等位基因 Alleles	中国黄羽鹌鹑 Chinese yellow quail	北京白羽鹌鹑 Beijing white quail	朝鲜鹌鹑 Korean quail
C71T	C	0.315	0.318	0.199	C215T	C	0.573	0.807	0.500
	T	0.685	0.682	0.801		T	0.427	0.193	0.500
C108T	C	0.667	0.660	0.647	A252G	A	0.323	0.364	0.354
	T	0.333	0.340	0.353		G	0.677	0.636	0.646
C168T	C	0.333	0.353	0.365	A279T	A	0.489	0.500	0.511
	T	0.667	0.647	0.635		T	0.511	0.500	0.489
C178T	C	0.667	0.647	0.635	C281T	C	0.010	0.023	0.178
	T	0.333	0.353	0.365		T	0.990	0.977	0.922
A184G	A	0.667	0.647	0.604	C293G	C	0.323	0.318	0.198
	G	0.333	0.353	0.396		G	0.677	0.682	0.902
C206T	C	0.344	0.364	0.458	C339T	C	0.906	0.886	0.781
	T	0.656	0.636	0.542		T	0.094	0.114	0.219
A209C	A	0.010	0.023	0.220	C458T	C	0.761	0.534	0.761
	C	0.990	0.977	0.780		T	0.239	0.466	0.239

位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C71T	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CT(n=51)	10.402 ±0.130 a	1.278 ±0.007 a	7.380 ±0.129 b	25.276 ±0.256 a	0.295 ±0.007 b	3.424 ±0.064 a	0.168 ±0.003 a	1.659 ±0.065 a
	TT(n=78)	10.551 ±0.095 a	1.286 ±0.005 a	7.376 ±0.118 b	25.610 ±0.203 a	0.287 ±0.007 b	3.476 ±0.032 a	0.172 ±0.002 a	1.618 ±0.059 a
C108T	CC(n=66)	10.544 ±0.098 a	1.287 ±0.006 a	7.491 ±0.122 a	25.417 ±0.209 a	0.293 ±0.008 a	3.465 ±0.032 a	0.170 ±0.002 a	1.633 ±0.064 a
	CT(n=56)	10.461 ±0.132 a	1.278 ±0.007 a	7.339 ±0.127 a	25.363 ±0.258 a	0.292 ±0.007 a	3.434 ±0.060 a	0.170 ±0.003 a	1.681 ±0.063 a
	TT(n=21)	10.443 ±0.160 a	1.269 ±0.011 a	7.500 ±0.225 a	25.540 ±0.405 a	0.297 ±0.013 a	3.457 ±0.086 a	0.173 ±0.004 a	1.590 ±0.118 a
C168T	CC(n=20)	10.415 ±0.166 a	1.265 ±0.011 a	7.535 ±0.234 a	25.401 ±0.400 a	0.300 ±0.013 a	3.435 ±0.087 a	0.175 ±0.003 a	1.605 ±0.123 a
	CT(n=60)	10.443 ±0.126 a	1.278 ±0.006 a	7.308 ±0.121 a	25.444 ±0.246 a	0.290 ±0.006 a	3.442 ±0.057 a	0.170 ±0.002 a	1.659 ±0.061 a
	TT(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
C178T	CC(n=63)	10.573 ±0.100 a	1.288 ±0.006 a	7.519 ±0.126 a	25.389 ±0.219 a	0.294 ±0.008 a	3.467 ±0.033 a	0.171 ±0.002 a	1.646 ±0.065 a
	CT(n=58)	10.419 ±0.129 a	1.277 ±0.007 a	7.326 ±0.125 a	25.348 ±0.245 a	0.292 ±0.007 a	3.421 ±0.057 a	0.170 ±0.002 a	1.678 ±0.061 a
	TT(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
A184G	AA(n=62)	10.589 ±0.100 a	1.288 ±0.006 a	7.526 ±0.128 a	25.413 ±0.221 a	0.294 ±0.008 a	3.474 ±0.033 a	0.171 ±0.002 a	1.646 ±0.066 a
	AG(n=59)	10.405 ±0.127 a	1.277 ±0.006 a	7.322 ±0.122 a	25.323 ±0.242 a	0.292 ±0.006 a	3.414 ±0.056 a	0.170 ±0.002 a	1.677 ±0.060 a
	GG(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
C206T	CC(n=22)	10.482 ±0.158 a	1.269 ±0.011 a	7.468 ±0.217 a	25.656 ±0.404 a	0.295 ±0.012 a	3.491 ±0.089 a	0.173 ±0.003 a	1.559 ±0.116 a
	CT(n=65)	10.403 ±0.121 a	1.277 ±0.006 a	7.280 ±0.116 a	25.385 ±0.238 a	0.290 ±0.006 a	3.426 ±0.052 a	0.170 ±0.002 a	1.669 ±0.056 a
	TT(n=56)	10.611 ±0.103 a	1.290 ±0.006 a	7.596 ±0.134 a	25.352 ±0.221 a	0.296 ±0.009 a	3.466 ±0.034 a	0.171 ±0.003 a	1.652 ±0.072 a
A209C	AA(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 b	28.080 ±0.210 a	0.228 ±0.013 b	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	AC(n=18)	10.717 ±0.234 a	1.290 ±0.009 a	6.700 ±0.245b	26.278 ±0.414b	0.257 ±0.012 ab	3.606 ±0.098 a	0.176 ±0.002 a	1.467 ±0.113 a
	CC(n=122)	10.470 ±0.077 a	1.280 ±0.004 a	7.566 ±0.083 a	25.220 ±0.158b	0.300 ±0.005 a	3.427 ±0.032 a	0.170 ±0.002 a	1.681 ±0.045 a
位点 Locus	基因型 Genotype	蛋质量 Egg weight/g	蛋形指数 Shape index	蛋黄高度 Yolk height/mm	蛋黄宽度 Yolk width/mm	蛋黄指数 Yolk index	蛋黄质量 Yolk weight/g	蛋壳厚度 Eggshell thickness/mm	蛋白高度 Protein height/mm
C215T	CC(n=60)	10.432 ±0.108 a	1.272 ±0.005 a	7.755 ±0.104 a	25.045 ±0.228 a	0.312 ±0.006 a	3.420 ±0.044 a	0.170 ±0.002 a	1.753 ±0.058 a
	CT(n=59)	10.553 ±0.122 a	1.293 ±0.007 a	7.239 ±0.119 b	25.660 ±0.231 a	0.284 ±0.006 b	3.493 ±0.053 a	0.173 ±0.003 a	1.577 ±0.069 a
	TT(n=24)	10.521 ±0.154 a	1.274 ±0.012 a	7.104 ±0.265 b	25.730 ±0.385 a	0.266 ±0.017 b	3.429 ±0.063 a	0.168 ±0.004 a	1.546 ±0.105 a
A252G	AA(n=20)	10.430 ±0.164 a	1.270 ±0.011 a	7.555 ±0.236 a	25.252 ±0.410 a	0.303 ±0.013 a	3.430 ±0.088 a	0.175 ±0.003 a	1.647 ±0.128 a
	AG(n=59)	10.459 ±0.126 a	1.279 ±0.007 a	7.319 ±0.122 a	25.494 ±0.245 a	0.290 ±0.006 a	3.444 ±0.058 a	0.169 ±0.002 a	1.648 ±0.061 a
	GG(n=64)	10.552 ±0.101 a	1.286 ±0.006 a	7.500 ±0.125 a	25.391 ±0.218 a	0.293 ±0.008 a	3.466 ±0.033 a	0.171 ±0.002 a	1.643 ±0.064 a
A279T	AA(n=14)	10.493 ±0.217 a	1.281 ±0.010 a	7.579 ±0.188 a	25.057 ±0.488 a	0.305 ±0.012 b	3.357 ±0.050 a	0.169 ±0.004 ab	1.773 ±0.127 a
	AT(n=115)	10.469 ±0.224 a	1.274 ±0.012 a	7.769 ±0.217 a	25.049 ±0.529 a	0.313 ±0.012 ab	3.425 ±0.112 a	0.164 ±0.005 b	1.801 ±0.110 a
	TT(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
C281T	CC(n=3)	10.267 ±0.504 a	1.272 ±0.046 a	6.400 ±0.400 a	28.080 ±0.210 a	0.228 ±0.013 a	3.533 ±0.233 a	0.171 ±0.015 a	1.287 ±0.375 a
	CT(n=14)	10.557 ±0.289 a	1.285 ±0.012 a	6.714 ±0.295 a	26.146 ±0.456 a	0.259 ±0.014 a	3.557 ±0.107 a	0.176 ±0.003 a	1.520 ±0.136 a
	TT(n=126)	10.495 ±0.075 a	1.281 ±0.004 a	7.537 ±0.083 a	25.269 ±0.158 a	0.298 ±0.005 a	3.438 ±0.032 a	0.170 ±0.002 a	1.668 ±0.044 a
C293G	CC(n=14)	10.536 ±0.203 a	1.263 ±0.012 a	7.943 ±0.195 a	24.820 ±0.461 a	0.323 ±0.012 a	3.421 ±0.103 a	0.176 ±0.004 a	1.752 ±0.137 a
	CG(n=52)	10.404 ±0.128 a	1.277 ±0.007 a	7.388 ±0.126 a	25.329 ±0.256 a	0.294 ±0.007 a	3.433 ±0.064 a	0.168 ±0.003 a	1.650 ±0.064 a
	GG(n=77)	10.552 ±0.097 a	1.286 ±0.005 a	7.370 ±0.119 a	25.579 ±0.203 a	0.287 ±0.007 a	3.470 ±0.032 a	0.172 ±0.002 a	1.623 ±0.060 a
C339T	CC(n=110)	10.509 ±0.079 a	1.280 ±0.005 a	7.499 ±0.090 a	25.300 ±0.173 a	0.296 ±0.006 a	3.439 ±0.035 a	0.170 ±0.002 a	1.659 ±0.047 a
	CT(n=25)	10.324 ±0.187 a	1.281 ±0.010 a	7.220 ±0.219 a	25.446 ±0.324 a	0.286 ±0.011 a	3.448 ±0.066 a	0.172 ±0.002 a	1.591 ±0.102 a
	TT(n=8)	10.863 ±0.397 a	1.293 ±0.017 a	7.188 ±0.371 a	26.869 ±0.636 a	0.269 ±0.019 a	3.638 ±0.116 a	0.173 ±0.006 a	1.636 ±0.217 a
C458T	CC(n=72)	10.488 ±0.101 a	1.276 ±0.006 a	7.269 ±0.125 a	25.472 ±0.217 a	0.284 ±0.008 a	3.443 ±0.049 a	0.171 ±0.002 a	1.581 ±0.060 a
	CT(n=52)	10.462 ±0.124 a	1.290 ±0.006 a	7.504 ±0.124 a	25.458 ±0.252 a	0.297 ±0.007 a	3.481 ±0.045 a	0.170 ±0.003 a	1.669 ±0.068 a
	TT(n=19)	10.626 ±0.197 a	1.274 ±0.008 a	7.858 ±0.175 a	25.072 ±0.384 a	0.316 ±0.010 a	3.405 ±0.049 a	0.173 ±0.004 a	1.825 ±0.111 a

鹌鹑GnRH-1基因多态性与蛋品质的关联分析

Association analysis between GnRH-1 gene polymorphism and egg quality in quail

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