贵州两地野生大厂茶嘌呤生物碱与儿茶素组分比较

doi:10.3969/j.issn.1004-1524.20230972

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (6): 1232-1244.DOI: 10.3969/j.issn.1004-1524.20230972

贵州两地野生大厂茶嘌呤生物碱与儿茶素组分比较

杨春¹(), 杨代星¹^,², 苏胜峰³, 梁思慧¹, 李燕¹, 郭燕¹, 乔大河¹, 密孝增¹, 陈正武¹^,^*()

1.贵州省农业科学院茶叶研究所,贵州贵阳 550006
2.贵州大学茶学院,贵州贵阳 550025
3.盘州市淤泥乡农村工作中心,贵州盘州 553523

收稿日期:2023-08-16 出版日期:2024-06-25 发布日期:2024-07-02
作者简介:杨春(1988—),女,湖南益阳人,硕士,副研究员,主要从事茶树资源育种与品种抗性研究。E-mail:610681998@qq.com
通讯作者: *陈正武,E-mail: zwchentea@163.com
基金资助:
贵州省科技计划(黔科合支撑〔2021〕一般189);贵州省科技计划(黔科合服企〔2020〕4016);贵州省农业科学院喀斯特山区作物基因资源与种业创新重点实验室项目(黔农科院种质资源〔2023〕03号);贵阳市科技计划(筑科合同〔2023〕2-1号)

Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China

YANG Chun¹(), YANG Daixing¹^,², SU Shengfeng³, LIANG Sihui¹, LI Yan¹, GUO Yan¹, QIAO Dahe¹, MI Xiaozeng¹, CHEN Zhengwu¹^,^*()

1. Tea Research Institute,Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
2. Tea College, Guizhou University, Guiyang 550025, China
3. Rural Work Center of Yuni Township, Panzhou County, Panzhou 553523, Guizhou, China

Received:2023-08-16 Online:2024-06-25 Published:2024-07-02

摘要/Abstract

摘要：

探明普安和盘州两地野生大厂茶生化差异,为贵州野生大厂茶资源的保护与开发利用提供科学依据,以普安和盘州两地40份野生大厂茶为材料,采用高效液相色谱法(HPLC)测定大厂茶春季一芽二叶新梢的嘌呤生物碱组分和儿茶素组分和含量,并结合聚类分析和正交偏最小二乘判别分析(OPLS-DA),对两地大厂茶种质资源进行类别区分和关键差异化合物筛选。结果表明,两地大厂茶种质资源中主要嘌呤生物碱不同,普安大厂茶为咖啡碱,盘州大厂茶为苦茶碱。表儿茶素没食子酸酯(epigallocatechin gallate, ECG)和表儿茶素(epicatechin,EC)是大厂茶中含量较突出的儿茶素组分。两地大厂茶的苦茶碱、咖啡碱、没食子酸、儿茶素(catechin, C)和表儿茶素(epicatechin, EC)含量存在极显著差异,普安大厂茶中咖啡碱和没食子酸含量平均值分别为28.53 mg·g^-1和0.29 mg·g^-1,极显著高于盘州大厂茶(2.92 mg·g^-1和0.13 mg·g^-1)。盘州大厂茶中苦茶碱、C和EC含量平均值分别是22.88、2.37、36.83 mg·g^-1,极显著高于普安大厂茶(1.58、1.81、26.55 mg·g^-1)。相关性分析显示,11个生化成分间相关性较强,有14对呈显著或极显著正相关,16对呈显著或极显著负相关。聚类分析结果显示:40份野生大厂茶可分为3类,第Ⅰ类为30份盘州大厂茶种质资源,主要生化特点是苦茶碱和C含量较高,咖啡碱含量较低;10份普安大厂茶资源被分作两类,GT-PA-03等7份种质资源构成第Ⅱ类,它们的咖啡碱、EC和ECG含量较高,苦茶碱、没食子酸、表没食子儿茶素(epigallocatechin, EGC)、C、表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)含量较低;GT-PA-01、GT-PA-02和GT-PA-09组成第Ⅲ类,它们的没食子酸、EGC和EGCG含量较高。OPLS-DA分析结果显示,咖啡碱、苦茶碱和没食子酸是区分普安和盘州两地大厂茶的关键生化因子。40份大厂茶种质资源中筛选出高苦茶碱(>25.0 mg·g^-1)种质3份,高没食子儿茶素没食子酸酯(gallocatechin gallate, GCG)(>15.0 mg·g^-1)种质2份,高ECG(>80.0 mg·g^-1)种质3份,高EC(>50.0 mg·g^-1)种质3份。

关键词: 普安, 盘州, 大厂茶, 苦茶碱, 咖啡碱

Abstract:

The objective of this study was not only to explore the biochemical differences between wild Camellia tachangensis in Pu’an County and Panzhou County, but also to provide the scientific basis for the protection, development and utilization of wild C. tachangensis in Guizhou. A total of 40 C. tachangensis germplasm resources from Pu’an County and Panzhou County were used as materials, the contents of theobromine, theophylline, theacrine, caffeine,gallic acid, epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epigallocatechin gallate (ECG) were determined by high performance liquid chromatography (HPLC). Cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to classify C. tachangensis germplasm resources from two regions and select key differential compounds. The results showed that the main purine alkaloids in C. tachangensis from Pu’an and Panzhou were caffeine and theacrine, respectively. ECG and EC were the prominent components of catechin in C. tachangensis.The contents of theacrine, caffeine, gallic acid, C and EC in C. tachangensis from two regions were significantly different. The contents of caffeine and gallic acid in C. tachangensis form Pu’an were average to 28.53 mg·g^-1 and 0.29 mg·g^-1, which were significantly higher than those from Panzhou. The contents of theacrine, C and EC in C. tachangensis from Panzhou were average to 22.88 mg·g^-1, 2.37 mg·g^-1 and 36.83 mg·g^-1, which were extremely significantly higher than those from Pu’an. The correlation analysis showed that there were strong correlation between 11 biochemical components, with 14 pairs showing significant or extremely significant positive correlations, 16 pairs showing significant or extremely significant negative correlations. Based on the hierarchical clustering of 11 biochemical components, 40 C. tachangensis germplasm resources could be divided into 3 groups. All 30 C. tachangensis germplasm resources from Panzhou were composed group Ⅰ and the main biochemical characteristics were high content of theacrine and C, and low content of caffeine; 10 C. tachangensis germplasm resources from Pu’an were separated into 2 categories, GT-PA-03 and other 6 germplasm resources constituted group Ⅱ,the main biochemical characteristics were high content of caffeine, EC, ECG, and low content of theacrine, gallic acid, EGC, C, EGCG; GT-PA-01, GT-PA-02 and GT-PA-09 were composed group Ⅲ and the main biochemical characteristics were high content of gallic acid, EGC and EGCG. OPLS-DA results showed that caffeine, theacrine and gallic acid were the key biochemical factors to distinguish C. tachangensis germplasm resources from Pu’an and Panzhou. A series of specific genetic resources were screened out from the 40 C. tachangensis germplasm resources, which included three high theacrine (>25.0 mg·g^-1) germplasm resources, two high GCG (>15.0 mg·g^-1) germplasm resources, three high ECG (>80.0 mg·g^-1) germplasm resources, three high EC (>50.0 mg·g^-1) germplasm resources.

Key words: Pu’an, Panzhou, Camellia tachangensis, theacrine, caffeine

中图分类号:

S571.1

杨春, 杨代星, 苏胜峰, 梁思慧, 李燕, 郭燕, 乔大河, 密孝增, 陈正武. 贵州两地野生大厂茶嘌呤生物碱与儿茶素组分比较[J]. 浙江农业学报, 2024, 36(6): 1232-1244.

YANG Chun, YANG Daixing, SU Shengfeng, LIANG Sihui, LI Yan, GUO Yan, QIAO Dahe, MI Xiaozeng, CHEN Zhengwu. Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1232-1244.

图/表 8

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材料编号	可可碱含量	茶碱含量	苦茶碱含量	咖啡碱含量
Material ID	Theobromine content	Theophylline content	Theacrine content	Caffeine content
GT-PA-01	14.18±0.62	0.48±0.01	N.D.	25.85±1.13
GT-PA-02	6.88±0.14	0.57±0.03	N.D.	30.04±0.91
GT-PA-03	10.81±0.12	0.56±0.01	N.D.	37.75±0.12
GT-PA-04	6.54±0.08	0.53±0.01	N.D.	26.35±0.38
GT-PA-05	12.15±0.15	0.59±0.01	N.D.	31.40±0.17
GT-PA-06	14.48±0.18	0.60±0.01	N.D.	34.95±0.23
GT-PA-07	10.43±0.10	0.60±0.01	N.D.	29.54±0.37
GT-PA-08	13.16±0.20	0.46±0.01	15.77±0.07	10.79±0.08
GT-PA-09	8.10±0.03	0.48±0.01	N.D.	24.38±0.09
GT-PA-10	15.27±0.25	0.74±0.01	N.D.	34.22±0.22
范围Range	6.54~15.27	0.46~0.74	0~15.77	10.79~37.75
平均值Mean	11.20	0.56	1.58	28.53
GT-PZ-01	6.86±0.15	0.39±0.01	23.47±0.21	2.05±0.05
GT-PZ-02	10.98±0.10	0.61±0.01	26.05±0.32	4.50±0.13
GT-PZ-03	8.77±0.01	0.58±0.01	20.78±0.17	1.59±0.02
GT-PZ-04	5.60±0.02	0.42±.001	22.97±0.34	0.88±0.02
GT-PZ-05	6.38±0.08	0.34±0.01	20.08±0.11	1.31±0.03
GT-PZ-06	8.95±0.08	0.47±0.01	22.20±0.01	2.24±0.06
GT-PZ-07	5.59±0.01	0.42±0.01	20.91±0.07	0.90±0.01
GT-PZ-08	4.40±0.02	0.34±0.01	23.23±0.32	0.66±0.01
GT-PZ-09	7.02±0.01	0.47±0.01	23.86±0.05	1.53±0.01
GT-PZ-10	11.19±0.09	0.96±0.02	24.37±0.24	4.25±0.04
GT-PZ-11	11.31±0.32	0.50±0.02	22.58±0.19	2.97±0.12
GT-PZ-12	12.83±0.21	0.60±0.01	23.31±0.17	3.66±0.06
GT-PZ-13	13.92±0.15	0.52±0.01	24.08±0.17	5.78±0.07
GT-PZ-14	11.66±0.68	0.52±0.03	22.65±1.17	4.46±0.26
GT-PZ-15	10.20±0.20	0.57±0.01	24.08±0.47	3.93±0.09
GT-PZ-16	4.91±0.19	0.36±0.01	20.73±0.68	0.86±0.04
GT-PZ-17	12.09±0.66	0.34±0.01	20.80±1.02	2.18±0.12
GT-PZ-18	9.67±0.12	0.68±0.02	27.96±0.71	2.50±0.03
GT-PZ-19	6.34±0.32	0.56±0.02	21.23±1.02	1.97±0.09
GT-PZ-20	13.01±0.16	0.79±0.03	22.51±0.06	4.69±0.04
GT-PZ-21	5.54±0.19	0.53±0.01	19.73±0.69	1.35±0.04
GT-PZ-22	4.19±0.07	0.30±0.01	20.15±0.33	0.72±0.04
GT-PZ-23	14.09±0.53	0.43±0.01	21.66±0.82	4.33±0.16
GT-PZ-24	8.52±0.03	0.42±0.01	23.30±0.06	2.35±0.01
GT-PZ-25	13.60±0.30	0.49±0.01	23.04±0.34	4.56±0.07
GT-PZ-26	14.15±0.16	0.41±0.01	23.67±0.05	3.83±0.04
GT-PZ-27	4.99±0.05	0.48±.001	24.57±0.07	4.63±0.02
GT-PZ-28	9.86±0.57	0.50±0.01	20.81±1.10	3.79±0.25
GT-PZ-29	8.79±0.04	0.80±0.01	27.47±0.28	3.93±0.06
GT-PZ-30	8.74±0.36	0.62±0.01	24.12±0.70	5.07±0.47
范围Range	4.19~14.15	0.30~0.96	19.73~27.96	0.66~5.78
平均值Mean	9.14	0.51	22.88	2.92
F值F value	0.010	2.208	2.426^**	18.735^**

材料编号	可可碱含量	茶碱含量	苦茶碱含量	咖啡碱含量
Material ID	Theobromine content	Theophylline content	Theacrine content	Caffeine content
GT-PA-01	14.18±0.62	0.48±0.01	N.D.	25.85±1.13
GT-PA-02	6.88±0.14	0.57±0.03	N.D.	30.04±0.91
GT-PA-03	10.81±0.12	0.56±0.01	N.D.	37.75±0.12
GT-PA-04	6.54±0.08	0.53±0.01	N.D.	26.35±0.38
GT-PA-05	12.15±0.15	0.59±0.01	N.D.	31.40±0.17
GT-PA-06	14.48±0.18	0.60±0.01	N.D.	34.95±0.23
GT-PA-07	10.43±0.10	0.60±0.01	N.D.	29.54±0.37
GT-PA-08	13.16±0.20	0.46±0.01	15.77±0.07	10.79±0.08
GT-PA-09	8.10±0.03	0.48±0.01	N.D.	24.38±0.09
GT-PA-10	15.27±0.25	0.74±0.01	N.D.	34.22±0.22
范围Range	6.54~15.27	0.46~0.74	0~15.77	10.79~37.75
平均值Mean	11.20	0.56	1.58	28.53
GT-PZ-01	6.86±0.15	0.39±0.01	23.47±0.21	2.05±0.05
GT-PZ-02	10.98±0.10	0.61±0.01	26.05±0.32	4.50±0.13
GT-PZ-03	8.77±0.01	0.58±0.01	20.78±0.17	1.59±0.02
GT-PZ-04	5.60±0.02	0.42±.001	22.97±0.34	0.88±0.02
GT-PZ-05	6.38±0.08	0.34±0.01	20.08±0.11	1.31±0.03
GT-PZ-06	8.95±0.08	0.47±0.01	22.20±0.01	2.24±0.06
GT-PZ-07	5.59±0.01	0.42±0.01	20.91±0.07	0.90±0.01
GT-PZ-08	4.40±0.02	0.34±0.01	23.23±0.32	0.66±0.01
GT-PZ-09	7.02±0.01	0.47±0.01	23.86±0.05	1.53±0.01
GT-PZ-10	11.19±0.09	0.96±0.02	24.37±0.24	4.25±0.04
GT-PZ-11	11.31±0.32	0.50±0.02	22.58±0.19	2.97±0.12
GT-PZ-12	12.83±0.21	0.60±0.01	23.31±0.17	3.66±0.06
GT-PZ-13	13.92±0.15	0.52±0.01	24.08±0.17	5.78±0.07
GT-PZ-14	11.66±0.68	0.52±0.03	22.65±1.17	4.46±0.26
GT-PZ-15	10.20±0.20	0.57±0.01	24.08±0.47	3.93±0.09
GT-PZ-16	4.91±0.19	0.36±0.01	20.73±0.68	0.86±0.04
GT-PZ-17	12.09±0.66	0.34±0.01	20.80±1.02	2.18±0.12
GT-PZ-18	9.67±0.12	0.68±0.02	27.96±0.71	2.50±0.03
GT-PZ-19	6.34±0.32	0.56±0.02	21.23±1.02	1.97±0.09
GT-PZ-20	13.01±0.16	0.79±0.03	22.51±0.06	4.69±0.04
GT-PZ-21	5.54±0.19	0.53±0.01	19.73±0.69	1.35±0.04
GT-PZ-22	4.19±0.07	0.30±0.01	20.15±0.33	0.72±0.04
GT-PZ-23	14.09±0.53	0.43±0.01	21.66±0.82	4.33±0.16
GT-PZ-24	8.52±0.03	0.42±0.01	23.30±0.06	2.35±0.01
GT-PZ-25	13.60±0.30	0.49±0.01	23.04±0.34	4.56±0.07
GT-PZ-26	14.15±0.16	0.41±0.01	23.67±0.05	3.83±0.04
GT-PZ-27	4.99±0.05	0.48±.001	24.57±0.07	4.63±0.02
GT-PZ-28	9.86±0.57	0.50±0.01	20.81±1.10	3.79±0.25
GT-PZ-29	8.79±0.04	0.80±0.01	27.47±0.28	3.93±0.06
GT-PZ-30	8.74±0.36	0.62±0.01	24.12±0.70	5.07±0.47
范围Range	4.19~14.15	0.30~0.96	19.73~27.96	0.66~5.78
平均值Mean	9.14	0.51	22.88	2.92
F值F value	0.010	2.208	2.426^**	18.735^**

材料编号 Material ID	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG	ECG
GT-PA-01	0.39±0.01	16.41±0.34	2.29±0.01	19.54±0.67	39.94±2.65	22.98±1.09	26.91±1.95
GT-PA-02	0.52±0.01	17.82±0.70	1.85±0.01	24.61±0.43	32.99±1.67	N.D.	28.16±1.63
GT-PA-03	0.20±0.01	N.D.	1.96±0.01	31.06±0.11	4.19±0.03	N.D.	70.87±0.47
GT-PA-04	0.19±0.01	6.99±0.25	2.06±0.01	36.35±0.44	6.34±0.06	N.D.	49.15±0.24
GT-PA-05	0.12±0.01	3.41±0.05	2.03±0.03	33.77±0.37	11.04±0.22	N.D.	71.37±2.83
GT-PA-06	0.19±0.01	N.D.	1.85±0.03	19.15±0.11	7.45±0.32	N.D.	81.30±6.95
GT-PA-07	0.33±0.01	N.D.	2.07±0.01	26.56±0.21	6.03±0.08	N.D.	74.56±1.82
GT-PA-08	0.19±0.01	N.D.	2.03±0.01	31.53±0.21	6.34±0.02	21.30±0.11	85.20±0.43
GT-PA-09	0.50±0.01	23.08±0.16	N.D.	16.94±0.03	37.05±1.41	N.D.	19.24±0.72
GT-PA-10	0.24±0.01	N.D.	1.98±0.09	25.99±0.08	6.67±0.06	N.D.	73.38±0.54
范围Range	0.12~0.52	0~23.08	0~2.29	16.94~36.35	4.19~39.94	0~22.98	19.24~85.20
平均值Mean	0.29	6.77	1.81	26.55	15.81	4.43	58.01
GT-PZ-01	0.07±0.01	2.55±0.01	2.29±0.01	47.84±0.58	6.18±0.19	N.D.	37.04±4.12
GT-PZ-02	0.17±0.01	1.88±0.04	2.70±0.01	40.74±0.22	6.36±0.10	N.D.	70.36±3.41
GT-PZ-03	0.12±0.01	3.20±0.05	2.63±0.01	42.53±0.36	7.43±0.18	N.D.	51.58±1.89
GT-PZ-04	0.08±0.01	2.82±0.08	2.19±0.04	39.14±0.73	5.51±0.01	N.D.	44.81±1.71
GT-PZ-05	0.08±0.01	1.82±0.01	2.85±0.03	46.34±0.77	5.91±0.19	N.D.	44.78±2.00
GT-PZ-06	0.09±0.01	3.04±0.01	2.63±0.01	44.64±0.21	6.90±0.12	N.D.	63.27±2.53
GT-PZ-07	0.07±0.01	2.49±0.01	2.37±0.01	51.95±0.07	6.60±0.15	N.D.	51.04±2.65
GT-PZ-08	0.07±0.01	2.89±0.01	2.14±0.01	42.91±0.42	5.64±0.06	N.D.	26.80±0.52
GT-PZ-09	0.07±0.01	2.16±0.01	2.56±0.02	48.30±0.42	6.10±0.01	N.D.	48.82±2.46
GT-PZ-10	0.17±0.01	2.39±0.05	2.31±0.01	31.20±0.34	7.65±0.08	N.D.	63.64±1.06
GT-PZ-11	0.18±0.01	1.53±0.08	2.09±0.01	24.42±0.34	6.87±0.02	N.D.	60.82±2.77
GT-PZ-12	0.16±0.01	4.27±0.05	2.35±0.04	33.78±0.19	9.11±0.03	N.D.	73.08±0.04
GT-PZ-13	0.28±0.01	1.97±0.03	2.00±0.01	20.70±0.83	8.73±0.22	N.D.	59.16±1.16
GT-PZ-14	0.14±0.01	2.20±0.10	2.54±0.06	28.28±1.10	6.32±0.07	N.D.	52.32±0.62
GT-PZ-15	0.18±0.01	3.06±0.04	2.40±0.03	25.15±0.49	7.84±0.29	N.D.	51.51±4.52
GT-PZ-16	0.07±0.01	2.14±0.08	2.75±0.09	48.15±1.87	5.78±0.11	N.D.	37.24±2.53
GT-PZ-17	0.14±0.01	3.13±0.03	2.57±0.06	46.03±2.07	7.20±0.32	N.D.	68.09±6.57
GT-PZ-18	0.11±0.01	4.27±0.42	2.00±0.01	23.53±0.68	9.41±0.42	N.D.	43.95±3.64
GT-PZ-19	0.07±0.01	5.01±0.23	2.74±0.08	51.57±3.26	5.07±0.17	N.D.	49.39±9.05
GT-PZ-20	0.17±0.01	3.16±0.14	2.34±0.01	28.24±0.12	7.33±0.06	N.D.	71.09±0.85
GT-PZ-21	0.07±0.01	1.45±0.04	2.34±0.03	40.60±1.23	5.02±0.04	N.D.	39.64±2.02
GT-PZ-22	0.04±0.01	1.56±0.06	2.02±0.01	53.81±1.44	4.84±0.01	N.D.	29.85±0.44
GT-PZ-23	0.27±0.01	2.11±0.07	2.06±0.01	24.54±0.84	9.55±0.30	N.D.	61.85±1.44
GT-PZ-24	0.09±0.01	0.92±0.01	2.59±0.01	41.23±0.24	5.40±0.06	N.D.	63.84±2.82
GT-PZ-25	0.19±0.01	2.01±0.03	2.29±0.01	23.92±0.22	6.66±0.18	N.D.	59.34±3.59
GT-PZ-26	0.17±0.01	4.56±0.02	2.12±0.01	25.98±0.13	6.53±0.05	N.D.	60.83±0.69
GT-PZ-27	0.11±0.01	3.91±0.33	2.29±0.01	37.77±0.47	6.36±0.04	N.D.	55.50±0.23
GT-PZ-28	0.17±0.01	1.55±0.01	2.25±0.05	28.51±0.72	6.25±0.01	N.D.	48.84±1.01
GT-PZ-29	0.19±.01	6.81±0.40	2.52±0.05	38.37±0.18	6.61±0.04	N.D.	83.16±1.67
GT-PZ-30	0.24±0.01	4.51±0.23	2.27±0.08	24.77±0.77	6.52±0.76	N.D.	66.16±1.68
范围Range	0.04~0.28	0.92~6.81	2.00~2.85	20.74~53.81	4.84~9.55	0	26.80~83.16
平均值Mean	0.13	2.85	2.37	36.83	6.72	0	54.59
F值F value	17.752^**	63.647	2.554^**	5.740^**	104.008	50.415	13.964

材料编号 Material ID	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG	ECG
GT-PA-01	0.39±0.01	16.41±0.34	2.29±0.01	19.54±0.67	39.94±2.65	22.98±1.09	26.91±1.95
GT-PA-02	0.52±0.01	17.82±0.70	1.85±0.01	24.61±0.43	32.99±1.67	N.D.	28.16±1.63
GT-PA-03	0.20±0.01	N.D.	1.96±0.01	31.06±0.11	4.19±0.03	N.D.	70.87±0.47
GT-PA-04	0.19±0.01	6.99±0.25	2.06±0.01	36.35±0.44	6.34±0.06	N.D.	49.15±0.24
GT-PA-05	0.12±0.01	3.41±0.05	2.03±0.03	33.77±0.37	11.04±0.22	N.D.	71.37±2.83
GT-PA-06	0.19±0.01	N.D.	1.85±0.03	19.15±0.11	7.45±0.32	N.D.	81.30±6.95
GT-PA-07	0.33±0.01	N.D.	2.07±0.01	26.56±0.21	6.03±0.08	N.D.	74.56±1.82
GT-PA-08	0.19±0.01	N.D.	2.03±0.01	31.53±0.21	6.34±0.02	21.30±0.11	85.20±0.43
GT-PA-09	0.50±0.01	23.08±0.16	N.D.	16.94±0.03	37.05±1.41	N.D.	19.24±0.72
GT-PA-10	0.24±0.01	N.D.	1.98±0.09	25.99±0.08	6.67±0.06	N.D.	73.38±0.54
范围Range	0.12~0.52	0~23.08	0~2.29	16.94~36.35	4.19~39.94	0~22.98	19.24~85.20
平均值Mean	0.29	6.77	1.81	26.55	15.81	4.43	58.01
GT-PZ-01	0.07±0.01	2.55±0.01	2.29±0.01	47.84±0.58	6.18±0.19	N.D.	37.04±4.12
GT-PZ-02	0.17±0.01	1.88±0.04	2.70±0.01	40.74±0.22	6.36±0.10	N.D.	70.36±3.41
GT-PZ-03	0.12±0.01	3.20±0.05	2.63±0.01	42.53±0.36	7.43±0.18	N.D.	51.58±1.89
GT-PZ-04	0.08±0.01	2.82±0.08	2.19±0.04	39.14±0.73	5.51±0.01	N.D.	44.81±1.71
GT-PZ-05	0.08±0.01	1.82±0.01	2.85±0.03	46.34±0.77	5.91±0.19	N.D.	44.78±2.00
GT-PZ-06	0.09±0.01	3.04±0.01	2.63±0.01	44.64±0.21	6.90±0.12	N.D.	63.27±2.53
GT-PZ-07	0.07±0.01	2.49±0.01	2.37±0.01	51.95±0.07	6.60±0.15	N.D.	51.04±2.65
GT-PZ-08	0.07±0.01	2.89±0.01	2.14±0.01	42.91±0.42	5.64±0.06	N.D.	26.80±0.52
GT-PZ-09	0.07±0.01	2.16±0.01	2.56±0.02	48.30±0.42	6.10±0.01	N.D.	48.82±2.46
GT-PZ-10	0.17±0.01	2.39±0.05	2.31±0.01	31.20±0.34	7.65±0.08	N.D.	63.64±1.06
GT-PZ-11	0.18±0.01	1.53±0.08	2.09±0.01	24.42±0.34	6.87±0.02	N.D.	60.82±2.77
GT-PZ-12	0.16±0.01	4.27±0.05	2.35±0.04	33.78±0.19	9.11±0.03	N.D.	73.08±0.04
GT-PZ-13	0.28±0.01	1.97±0.03	2.00±0.01	20.70±0.83	8.73±0.22	N.D.	59.16±1.16
GT-PZ-14	0.14±0.01	2.20±0.10	2.54±0.06	28.28±1.10	6.32±0.07	N.D.	52.32±0.62
GT-PZ-15	0.18±0.01	3.06±0.04	2.40±0.03	25.15±0.49	7.84±0.29	N.D.	51.51±4.52
GT-PZ-16	0.07±0.01	2.14±0.08	2.75±0.09	48.15±1.87	5.78±0.11	N.D.	37.24±2.53
GT-PZ-17	0.14±0.01	3.13±0.03	2.57±0.06	46.03±2.07	7.20±0.32	N.D.	68.09±6.57
GT-PZ-18	0.11±0.01	4.27±0.42	2.00±0.01	23.53±0.68	9.41±0.42	N.D.	43.95±3.64
GT-PZ-19	0.07±0.01	5.01±0.23	2.74±0.08	51.57±3.26	5.07±0.17	N.D.	49.39±9.05
GT-PZ-20	0.17±0.01	3.16±0.14	2.34±0.01	28.24±0.12	7.33±0.06	N.D.	71.09±0.85
GT-PZ-21	0.07±0.01	1.45±0.04	2.34±0.03	40.60±1.23	5.02±0.04	N.D.	39.64±2.02
GT-PZ-22	0.04±0.01	1.56±0.06	2.02±0.01	53.81±1.44	4.84±0.01	N.D.	29.85±0.44
GT-PZ-23	0.27±0.01	2.11±0.07	2.06±0.01	24.54±0.84	9.55±0.30	N.D.	61.85±1.44
GT-PZ-24	0.09±0.01	0.92±0.01	2.59±0.01	41.23±0.24	5.40±0.06	N.D.	63.84±2.82
GT-PZ-25	0.19±0.01	2.01±0.03	2.29±0.01	23.92±0.22	6.66±0.18	N.D.	59.34±3.59
GT-PZ-26	0.17±0.01	4.56±0.02	2.12±0.01	25.98±0.13	6.53±0.05	N.D.	60.83±0.69
GT-PZ-27	0.11±0.01	3.91±0.33	2.29±0.01	37.77±0.47	6.36±0.04	N.D.	55.50±0.23
GT-PZ-28	0.17±0.01	1.55±0.01	2.25±0.05	28.51±0.72	6.25±0.01	N.D.	48.84±1.01
GT-PZ-29	0.19±.01	6.81±0.40	2.52±0.05	38.37±0.18	6.61±0.04	N.D.	83.16±1.67
GT-PZ-30	0.24±0.01	4.51±0.23	2.27±0.08	24.77±0.77	6.52±0.76	N.D.	66.16±1.68
范围Range	0.04~0.28	0.92~6.81	2.00~2.85	20.74~53.81	4.84~9.55	0	26.80~83.16
平均值Mean	0.13	2.85	2.37	36.83	6.72	0	54.59
F值F value	17.752^**	63.647	2.554^**	5.740^**	104.008	50.415	13.964

成分 Component	可可碱 Theobromine	茶碱 Theophylline	苦茶碱 Theacrine	咖啡碱 Caffeine	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG
茶碱Theophylline	0.370^*
苦茶碱Theacrine	-0.200	-0.091
咖啡碱Caffeine	0.341^*	0.270	-0.953^**
没食子酸	0.382^*	0.240	-0.585^**	0.603^**
Gallic acid
EGC	-0.102	0.012	-0.383^*	0.293	0.701^**
C	-0.117	-0.034	0.525^**	-0.506^**	-0.635^**	-0.566^**
EC	-0.678^**	-0.395^*	0.369^*	-0.492^**	-0.733^**	-0.314^*	0.555^**
EGCG	0.115	0.009	-0.506^**	0.424^**	0.783^**	0.918^**	-0.556^**	-0.452^**
GCG	0.286	-0.093	-0.236	0.182	0.258	0.228	-0.034	-0.200	0.422^**
ECG	0.594^**	0.459^**	0.031	0.156	-0.086	-0.540^**	0.242	-0.168	-0.481^**	-0.007

贵州两地野生大厂茶嘌呤生物碱与儿茶素组分比较

Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China

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生化成分 Biochemical composition	第Ⅰ类 Group Ⅰ		第Ⅱ类 Group Ⅱ		第Ⅲ类 Group Ⅲ
生化成分 Biochemical composition	含量 Content	平均值 Average	含量 Content	平均值 Average	含量 Content	平均值 Average
可可碱Theobromine	4.19~14.15	9.14±3.20 aA	6.54~15.27	11.83±2.94 aA	6.88~15.27	12.11±4.56 aA
茶碱Theophylline	0.30~0.96	0.51±0.15 aA	0.46~0.74	0.59±0.08 aA	0.48~0.74	0.60±0.13 aA
苦茶碱Theacrine	19.73~27.96	22.88±2.04 aA	0~15.77	2.25±5.96 bB	0	0 bB
咖啡碱Caffeine	0.66~5.78	2.92±1.54 bB	10.79~37.75	29.29±8.97 aA	25.85~34.22	30.03±4.18 aA
没食子酸Gallic acid	0.04~0.28	0.13±0.06 bB	0.12~0.33	0.21±0.07 bB	0.24~0.52	0.38±0.14 aA
EGC	0.92~6.81	2.85±1.29 bB	0~6.99	1.49±2.74 bB	0~17.82	11.41±9.91 aA
C	2.00~2.85	2.37±0.24 aA	1.85~2.07	2.00±0.08 bB	1.85~2.29	2.04±0.23 bAB
EC	20.74~53.81	36.83±10.24 aA	19.15~36.35	29.20±5.76 abA	19.54~25.99	23.38±3.40 bA
EGCG	4.84~9.55	6.72±1.24 bB	4.19~11.04	6.87±2.09 bB	6.67~39.94	26.53±17.55 aA
GCG	0	0 bB	0~21.30	3.04±8.05 abAB	0~22.98	7.66±13.27 aA
ECG	26.80~83.16	54.59±13.22 bAB	49.15~85.20	72.26±11.49 aA	26.91~73.38	42.81±26.48 bB