2个牡丹切花品种核型、花药形态与花粉特性的比较

doi:10.3969/j.issn.1004-1524.20240096

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (2): 329-337.DOI: 10.3969/j.issn.1004-1524.20240096

2个牡丹切花品种核型、花药形态与花粉特性的比较

张欢欢(), 范挺秀, 高双成, 范丙友, 史国安()

河南科技大学牡丹学院,洛阳市牡丹生物学重点实验室,河南洛阳 471023

收稿日期:2024-01-25 出版日期:2025-02-25 发布日期:2025-03-20
作者简介:*史国安,E-mail:gashi1963@163.com
张欢欢(1997—),男,河南灵宝人,硕士,主要从事牡丹生物学研究。E-mail: ZHY197012@163.com
通讯作者: 史国安
基金资助:
国家重点研发计划项目(2018YFD1000400);国家自然科学基金(31372098);河南省自然科学基金(162300410075);河南省自然科学基金(202000410137)

Comparative analysis of karyotype, anther morphology and pollen characteristics for two varieties of tree peony cut flower

ZHANG Huanhuan(), FAN Tingxiu, GAO Shuangcheng, FAN Bingyou, SHI Guo’an()

Luoyang Key Laboratory of Peony Biology, College of Tree Peony, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2024-01-25 Online:2025-02-25 Published:2025-03-20
Contact: SHI Guo’an

摘要/Abstract

摘要： 为探明牡丹切花花朵开放后的花粉育性水平,分析花粉微观形态、代谢与花粉活力之间的关系。以2个切花牡丹品种凤丹和黄冠为试验材料,利用染色体压片法观测体细胞染色体核型、测微尺法测定花药形态、扫描电镜观测花粉形态、液体培育法测定花粉萌发率和分光光度法测定花粉萌发相关生理代谢指标差异。结果表明,凤丹为二倍体核型,单朵花粉量达1 262万粒,花粉粒形态饱满呈现为三裂球形,在25 ℃模拟温度条件下干燥后的花粉萌发率超过50%;黄冠为三倍体核型,单朵花粉量达288万粒,花粉粒形态异常为三裂三角形,畸形率高达97.2%,花粉萌发率为0%。黄冠花粉超氧化物歧化酶(SOD)活性、抗坏血酸(AsA)和可溶性蛋白质含量分别比凤丹降低了46.7%、39.3%和31.8%。黄冠花粉形态异常、蛋白质和膜脂过氧化代谢失调,可能是导致花粉不能萌发的重要原因。研究结果证实了黄冠牡丹是同源三倍体,花粉畸形、数量少、萌发活力低,用作切花可以降低插花过程中花粉污染造成的潜在风险。

关键词: 牡丹, 切花, 染色体倍性, 核型分析, 花粉活力

Abstract:

In this study, we examined the post-blooming pollen fertility of two cut tree peony varieties, Fengdan and Huangguan, focusing on its relationship among pollen micro-morphology, metabolism, and viability. We employed the chromosome squash technique to observe nuclear karyotypes, micrometer measurements for anther morphology, and scanning electron microscopy for pollen morphology. Additionally, we used liquid culture and spectrophotometry to assess pollen germination rates and related metabolic indicators. Our results indicated that Fengdan was a diploid karyotype and produced approximately 12.62 million rounded pollen grains per flower, characterized by three fissures and a deformity rate of 97.2%. This variety exhibited over 50% germination at a simulated temperature of 25 ℃. In contrast, Huangguan possessed a triploid karyotype with around 2.88 million abnormally shaped triangular pollen grains per flower, resulting in a zero of germination rate. Relative to Fengdan, Huangguan showed significant reductions in superoxide dismutase activity, ascorbic acid content, and soluble protein levels by 46.7%, 39.3%, and 31.8%, respectively. The abnormal pollen morphology and metabolic disorders in Huangguan likely contribute to its germination failure. Overall, Huangguan variety is characterized by low pollen quantity and viability, minimizing the risk of pollen contamination in cut flower arrangements.

Key words: tree peony, cut flower, chromosome ploidy, karyotyping, pollen vitality

中图分类号:

S685.11

张欢欢, 范挺秀, 高双成, 范丙友, 史国安. 2个牡丹切花品种核型、花药形态与花粉特性的比较[J]. 浙江农业学报, 2025, 37(2): 329-337.

ZHANG Huanhuan, FAN Tingxiu, GAO Shuangcheng, FAN Bingyou, SHI Guo’an. Comparative analysis of karyotype, anther morphology and pollen characteristics for two varieties of tree peony cut flower[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 329-337.

图/表 8

参考文献 49

[1]	MORIMOTO T, MATSUDA Y, SEKIGUCHI R, et al. Comprehensive assessment of intergeneric cross-compatibility of six fruit tree species in the tribe maleae (Rosaceae) based on in vivo pollen tube growth and field pollination[J]. The Horticulture Journal, 2023, 92(1): 13-21.
[2]	张鹏飞, 张道荣, 凌冬, 等. 植物花粉败育的研究进展[J]. 湖北农业科学, 2019, 58(23): 10-15.
	ZHANG P F, ZHANG D R, LING D, et al. Research progress of plant pollen abortion[J]. Hubei Agricultural Sciences, 2019, 58(23): 10-15. (in Chinese with English abstract)
[3]	沈春琳, 许慧慧, 袁东, 等. 空气致敏花粉污染与支气管哮喘的研究进展[J]. 环境与职业医学, 2011, 28(1): 56-58, 62.
	SHEN C L, XU H H, YUAN D, et al. Advances in researches on airborne allergenic pollen and bronchial asthma[J]. Journal of Environmental & Occupational Medicine, 2011, 28(1): 56-58, 62. (in Chinese with English abstract)
[4]	D’AMATO G, CECCHI L, BONINI S, et al. Allergenic pollen and pollen allergy in Europe[J]. Allergy, 2007, 62(9): 976-990.
[5]	廖凤林. 花粉污染及其防治[J]. 环境科学动态, 1992, 17(2): 5-7.
	LIAO F L. Pollen pollution and its control[J]. Environment and Sustainable Development, 1992, 17(2): 5-7. (in Chinese)
[6]	吴美娇, 张亚明, 王雪倩, 等. 无花粉污染百合的杂交育种研究[J]. 南京农业大学学报, 2019, 42 (6): 1030-1039.
	WU M J, ZHANG Y M, WANG X Q, et al. Study on hybrid breeding of pollen-free lily[J]. Journal of Nanjing Agricultural University, 2009, 42 (6): 1030-1039. (in Chinese with English abstract)
[7]	DONG T T, WANG L X, WANG R, et al. Transcriptomic analysis reveals candidate genes associated with anther development in Lilium Oriental Hybrid ‘Siberia’[J]. Frontiers in Plant Science, 2023, 14: 1128911.
[8]	罗浩, 成仿云, 郭鑫, 等. 基于灰色关联度分析法评价筛选紫斑牡丹切花品种[J]. 园艺学报, 2020, 47(11): 2169-2180.
	LUO H, CHENG F Y, GUO X, et al. Evaluation and selection of cut-flower cultivars based on grey relational grade analysis in flare tree peony (Paeonia rockii)[J]. Acta Horticulturae Sinica, 2020, 47(11): 2169-2180. (in Chinese with English abstract)
[9]	王怡晨, 孙海燕, 李永荣, 等. 油用牡丹‘凤丹’单株结实量及产油品质分析[J]. 南京林业大学学报(自然科学版), 2019, 43(4): 155-160.
	WANG Y C, SUN H Y, LI Y R, et al. Analysis of the variation in yield and oil quality traits of selected Paeonia ostii ‘Feng Dan’ individuals[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2019, 43(4): 155-160. (in Chinese with English abstract)
[10]	曹满, 施江, 史国安. 低温锻炼对冰温贮藏牡丹切花抗冷性的影响[J]. 西北植物学报, 2017, 37(10): 1996-2002.
	CAO M, SHI J, SHI G A. Effects of low-temperature acclimation on cold resistance of tree peony cut flowers in ice temperature storage[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(10): 1996-2002. (in Chinese with English abstract)
[11]	邱永杰, 何松林, 张翔宇, 等. 外源ASA、H₂O₂对牡丹切花衰老过程中生理活性及细胞学特性的影响[J]. 山东农业科学, 2023, 55(1): 75-83.
	QIU Y J, HE S L, ZHANG X Y, et al. Effects of exogenous ASA and H₂O₂ on physiological activity and cytological characteristics of cut tree peony flowers during senescence[J]. Shandong Agricultural Sciences, 2023, 55(1): 75-83. (in Chinese with English abstract)
[12]	施江, 王小燕, 张淑玲, 等. 培养基组分对牡丹花粉萌发和花粉管生长的影响[J]. 河南科技大学学报(自然科学版), 2013, 34(2): 75-80, 1.
	SHI J, WANG X Y, ZHANG S L, et al. Effect of medium components on pollen germination and tube growth of tree peony(Paeonia suffruticosa)[J]. Journal of Henan University of Science & Technology(Natural Science), 2013, 34(2): 75-80, 1. (in Chinese with English abstract)
[13]	盖树鹏, 盖伟玲, 郑国生. 不同条件下牡丹花粉的贮藏寿命[J]. 北方园艺, 2011(18): 104-106.
	GAI S P, GAI W L, ZHENG G S. Storage longevity of tree peony pollen in different conditions[J]. Northern Horticulture, 2011(18): 104-106. (in Chinese with English abstract)
[14]	孙珊珊, 王亦周, 王淑娟, 等. 凤丹牡丹花粉和花药壁营养成分分析及评价[J]. 核农学报, 2023, 37(4): 781-792.
	SUN S S, WANG Y Z, WANG S J, et al. Analysis and evaluation of nutritional components in pollen and anther wall of tree peony(Paeonia ostii Fengdan)[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(4): 781-792. (in Chinese with English abstract)
[15]	李懋学, 张斅方. 三倍体牡丹的细胞遗传学观察[J]. 遗传, 1982, 4(5): 19-21, 27.
	LI M X, ZHANG X F. A cytogenetic observation on tri-ploid paconia suffruticosa[J]. Hereditas(Beijing), 1982, 4(5): 19-21, 27. (in Chinese with English abstract)
[16]	刘淑敏, 王莲英, 秦魁杰, 等. 牡丹三倍体品种: 首案红[J]. 北京林学院学报, 1983, 5(4): 62-67.
	LIU S M, WANG L Y, QIN K J, et al. ‘Shou An Hong’, a triploid cultivar of tree peony(Paeonia suffruticosa)[J]. Journal of Beijing Forestry University, 1983, 5(4): 62-67. (in Chinese with English abstract)
[17]	叶迪, 施江, 高双成, 等. 乙烯促进牡丹‘洛阳红’切花花瓣脱落与内源生长素的关联性分析[J]. 中国农业科学, 2021, 54(23): 5097-5109.
	YE D, SHI J, GAO S C, et al. Correlation analysis of auxin involved in the process of petal abscission of tree peony Luoyanghong cut flowers by ethylene promoting[J]. Scientia Agricultura Sinica, 2021, 54(23): 5097-5109. (in Chinese with English abstract)
[18]	于玲, 夏振平, 李玉舒, 等. 牡丹鲜切花采后衰老机理研究进展[J]. 现代农业科技, 2020(2): 103-104, 107.
	YU L, XIA Z P, LI Y S, et al. Research progress on post-harvest senescence mechanism for cut flowers of tree peony[J]. Modern Agricultural Science and Technology, 2020(2): 103-104, 107. (in Chinese with English abstract)
[19]	李坤彦, 杜明杰, 钟原, 等. ‘正午’牡丹染色体预处理方法优化和在核型分析中的应用[J]. 北京林业大学学报, 2021, 43(5): 118-126.
	LI K Y, DU M J, ZHONG Y, et al. Optimization of chromosome pretreatment method and its application in karyotype analysis of Paeonia×lemoinei ‘High Noon’[J]. Journal of Beijing Forestry University, 2021, 43(5): 118-126. (in Chinese with English abstract)
[20]	李懋学, 陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究, 1985, 3(4): 297-302.
	LI M X, CHEN R Y. A suggestion on the standardization of karyotype analysis in plants[J]. Journal of Wuhan Botanical Research, 1985, 3(4): 297-302. (in Chinese with English abstract)
[21]	王蓝青, 吴美娇, 王雪倩, 等. 95份百合种质资源花粉量的测定与散粉特性分析[J]. 南京农业大学学报, 2018, 41(6): 1018-1028.
	WANG L Q, WU M J, WANG X Q, et al. Determination of pollen quantity and features of pollen dispersal for 95 lily resources[J]. Journal of Nanjing Agricultural University, 2018, 41(6): 1018-1028. (in Chinese with English abstract)
[22]	宋静, 王娟, 杜春, 等. 不同海拔分布的滇牡丹种源花粉活力差异分析[J]. 植物生理学报, 2022, 58(9): 1675-1684.
	SONG J, WANG J, DU C, et al. Analysis on the difference of pollen viability of Paeonia delavayi provenances at different altitudes[J]. Plant Physiology Journal, 2022, 58(9): 1675-1684. (in Chinese with English abstract)
[23]	张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 4版. 北京: 高等教育出版社, 2009.
[24]	陈建勋, 王晓峰. 植物生理学实验指导[M]. 2版. 广州: 华南理工大学出版社, 2006.
[25]	CUI L T, CHEN T, ZHAO X, et al. Karyotype analysis, genomic and fluorescence in situ hybridization (GISH and FISH) reveal the ploidy and parental origin of chromosomes in Paeonia Itoh hybrids[J]. International Journal of Molecular Sciences, 2022, 23(19), 11406.
[26]	CHASE C D, GABAY-LAUGHNAN S. Cytoplasmic male sterility and fertility restoration by nuclear genes[M]// Molecular biology and biotechnology of plant organelles. Dordrecht: Springer Netherlands, 2007: 593-621.
[27]	奚晓军, 査倩, 蒋爱丽, 等. 三倍体葡萄‘夏黑’的花粉母细胞减数分裂行为观察及其育性研究[J]. 西北植物学报, 2015, 35(12): 2422-2427.
	Ⅺ X J, CHA Q, JIANG A L, et al. Meiotic chromosome behavior of pollen mother cells and gamete fertility in triploid grape ‘Summer black’[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(12): 2422-2427. (in Chinese with English abstract)
[28]	VAFAIE-TABAR M, CHANDRASHEKARAN S. Meiosis in a triploid hybrid of Gossypium: high frequency of secondary bipolar spindles at metaphase Ⅱ[J]. Journal of Genetics, 2007, 86(1): 45-49.
[29]	FARCO G E, DEMATTEIS M. Meiotic behavior and pollen fertility in triploid and tetraploid natural populations of Campuloclinium macrocephalum(Eupatorieae, Asteraceae)[J]. Plant Systematics and Evolution, 2014, 300(8): 1843-1852.
[30]	沈植国, 孙萌, 丁鑫, 等. 不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响[J]. 浙江农业学报, 2021, 33(2): 278-287.
	SHEN Z G, SUN M, DING X, et al. Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 278-287. (in Chinese with English abstract)
[31]	侯小改, 郭大龙, 张亚冰, 等. 部分牡丹品种的染色体核型分析[J]. 河南科技大学学报(自然科学版), 2012, 33(5): 83-87, 1.
	HOU X G, GUO D L, ZHANG Y B, et al. Chromosome karyotype analysis of partial cultivars in tree peony[J]. Journal of Henan University of Science & Technology(Natural Science), 2012, 33(5): 83-87, 1. (in Chinese with English abstract)
[32]	张赞平, 张益民. 栽培牡丹的染色体数目和核型变异[J]. 河南农业大学学报, 1989, 23(1): 48-52.
	ZHANG Z P, ZHANG Y M. The variation of chromosome numbers and karyotypes in culfivated Paeonia suffruticosa[J]. Journal of Henan Agricultural University, 1989, 23(1): 48-52. (in Chinese with English abstract)
[33]	张赞平, 侯小改. 杨山牡丹的核型分析[J]. 遗传, 1996(5): 3-6.
	ZHANG Z P, HOU X G. Karyotype analysis of Paeonia ostii[J]. Hereditas, 1996(5): 3-6. (in Chinese with English abstract)
[34]	刘春洋, 彭朝凤, 程世平, 等. 高温诱导‘凤丹’牡丹2n雌配子创制三倍体[J]. 园艺学报, 2023, 50(7): 1455-1466.
	LIU C Y, PENG C F, CHENG S P, et al. Creation of triploid germplasm in Paeonia ostii ‘Fengdan’ through 2n female gametes inducing with high temperature treatment[J]. Acta Horticulturae Sinica, 2023, 50(7): 1455-1466. (in Chinese with English abstract)
[35]	钟原, 杜明杰, 成仿云, 等. 牡丹异源四倍体核型和育性分析[J]. 园艺学报, 2023, 50(7): 1444-1454.
	ZHONG Y, DU M J, CHENG F Y, et al. Karyotype and fertility analysis of allotetraploids in tree peony(Paeonia section moutan)[J]. Acta Horticulturae Sinica, 2023, 50(7): 1444-1454. (in Chinese)
[36]	MORANT M, JØRGENSEN K, SCHALLER H, et al. CYP703 is an ancient cytochrome P450 in land plants catalyzing in-chain hydroxylation of lauric acid to provide building blocks for sporopollenin synthesis in pollen[J]. The Plant Cell, 2007, 19(5): 1473-1487.
[37]	陈贤丰, 梁承邺. 水稻不育花约中 H₂O₂ 积累与膜质过氧化加剧[J]. 植物生理学报, 1991, 17(1): 44-48.
	CHEN X F, LIANG C Y. Accumulation of hydrogen peroxide and lipid peroxidation in male sterile anthers of Oryza sativa[J]. Acta Phytophysiologic sinica, 1991, 17(1): 44-48. (in Chinese with English abstract)
[38]	赵萍, 唐红, 刘兴宇, 等. 紫斑牡丹单瓣与半重瓣花粉超微结构及生理生化特性比较[J]. 西北植物学报, 2022, 42 (3): 418-426.
	ZHAO P, TANG H, LIU X Y, et al. Comparison of ultrastructure and physiological and biochemical characteristics of single and semi-double pollen of Paeonia rockii[J]. Acta Botanica Boreali-Occidentalia Sinica, 2022, 42 (3): 418-426. (in Chinese with English abstract)
[39]	贾文庆, 郭英姿, 赵国栋, 等. 西藏黄牡丹花粉生活力、寿命及贮存生理机制研究[J]. 植物生理学报, 2021, 57(2): 501-511.
	JIA W Q, GUO Y Z, ZHAO G D, et al. Studies on the pollen viability, longevity and storage physiological mechanism of Paeonia lutea[J]. Plant Physiology Journal, 2021, 57(2): 501-511. (in Chinese with English abstract)
[40]	DU G C, XU J G, GAO C R, et al. Effect of low storage temperature on pollen viability of fifteen herbaceous peonies[J]. Biotechnology Reports, 2019, 21: e00309.
[41]	任瑞芬, 李泽迪, 姜雪茹, 等. AsA-GSH抗氧化系统在超低温保存后牡丹花粉活力下降中的作用机制[J]. 植物生理学报, 2021, 57(7): 1517-1526.
	REN R F, LI Z D, JIANG X R, et al. The mechanism of ASA-GSH antioxidant system in the decreasing of Paeonia suffruticosa pollen viability after cryopreservation[J]. Plant Physiology Journal, 2021, 57(7): 1517-1526. (in Chinese with English abstract)
[42]	WILLS-KARP M, SANTELIZ J, KARP C L. The germless theory of allergic disease: revisiting the hygiene hypothesis[J]. Nature Reviews Immunology, 2001, 1(1): 69-75.
[43]	ESTEVE C, MONTEALEGRE C, MARINA M, et al. Analysis of olive allergens[J]. Talanta, 2012, 92: 1-14.
[44]	DE DIOS ALCHÉ J, M’RANI-ALAOUI M, CASTRO A J, et al. Ole e 1, the major allergen from olive (Olea europaea L.) pollen, increases its expression and is released to the culture medium during in vitro germination[J]. Plant & Cell Physiology, 2004, 45(9): 1149-1157.
[45]	NAKAMURA R, TESHIMA R. Proteomics-based allergen analysis in plants[J]. Journal of Proteomics, 2013, 93: 40-49.
[46]	辛嘉楠, 欧阳志云, 郑华, 等. 城市中的花粉致敏植物及其影响因素[J]. 生态学报, 2007, 27(9): 3820-3827.
	XIN J N, OUYANG Z Y, ZHENG H, et al. Allergenic pollen plants and their influencing factors in urban area[J]. Acta Ecologica Sinica, 2007, 27(9): 3820-3827. (in Chinese with English abstract)
[47]	徐慧, 姚霞珍, 佟珂珂, 等. 3种牡丹花器官不同部位挥发性成分分析[J]. 南京林业大学学报(自然科学版), 2023, 47(3): 63-69, 1-5.
	XU H, YAO X Z, TONG K K, et al. Analysis of volatile components in different parts of flower organs of three species of tree peony[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2023, 47(3): 63-69, 1-5. (in Chinese with English abstract)
[48]	杨祎凡, 周婷, 范俊俊, 等. 基于切花应用的观赏海棠品种评价与筛选[J]. 南京林业大学学报(自然科学版), 2019, 43(4): 70-76.
	YANG Y F, ZHOU T, FAN J J, et al. Cultivar evaluation and selection in ornamental crabapple for cut flower application[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2019, 43(4): 70-76. (in Chinese with English abstract)
[49]	雷启义, 张廷进. 贵阳市主要切花花粉污染初步研究[J]. 黔东南民族师专学报, 1998, 16(5): 29-32.
	LEI Q Y, ZHANG T J. Preliminary research on pollution within Guiyang by pollens of magor cut flowers[J]. Journal of Southeast Guizhou National Teacher’s College, 1998, 16(5): 29-32. (in Chinese with English abstract)

品种 Varieties	染色体编号 Chromosome number	相对长度 Relative length/%	臂比 Arm ratio	着丝粒指数 Centromeric index/%	染色体类型 Chromosome type
凤丹Fengdan	A	9.88+13.55=23.43	1.37	72.91	m
	B	10.07+11.91=21.99	1.18	84.55	m
	C	9.77+11.31=21.08	1.16	86.38	m
	D	6.43+12.38=18.81	2.06	51.93	sm
	E	2.79+11.91=14.70	4.64	23.45	st
黄冠Huangguan	A	10.59+12.84=23.43	1.21	82.48	m
	B	9.77+11.62=21.39	1.19	84.08	m
	C	8.65+11.37=20.01	1.31	76.55	m
	D	6.44+12.87=19.31	2.00	50.01	sm
	E	3.10+12.76=15.87	4.11	24.29	st

品种 Varieties	染色体编号 Chromosome number	相对长度 Relative length/%	臂比 Arm ratio	着丝粒指数 Centromeric index/%	染色体类型 Chromosome type
凤丹Fengdan	A	9.88+13.55=23.43	1.37	72.91	m
	B	10.07+11.91=21.99	1.18	84.55	m
	C	9.77+11.31=21.08	1.16	86.38	m
	D	6.43+12.38=18.81	2.06	51.93	sm
	E	2.79+11.91=14.70	4.64	23.45	st
黄冠Huangguan	A	10.59+12.84=23.43	1.21	82.48	m
	B	9.77+11.62=21.39	1.19	84.08	m
	C	8.65+11.37=20.01	1.31	76.55	m
	D	6.44+12.87=19.31	2.00	50.01	sm
	E	3.10+12.76=15.87	4.11	24.29	st

品种 Varieties	花药数/个 Anthers per flower	花药长 Anther length/mm	花药宽 Anther width/mm	花药高 Anther height/mm	花药体积 Anther volume/mm³	花粉量 Pollen quantity per flower/10⁴
凤丹Fengdan	134.6±6.8 b	14.25±0.14 a	1.21±0.09 b	1.37±0.05 a	23.75±2.11 a	1 262.46±10.73 a
黄冠Huangguan	194.4±6.7 a	11.14±0.53 b	1.52±0.07 a	0.99±0.08 b	16.86±1.71 b	288.06±3.51 b

品种 Varieties	花药数/个 Anthers per flower	花药长 Anther length/mm	花药宽 Anther width/mm	花药高 Anther height/mm	花药体积 Anther volume/mm³	花粉量 Pollen quantity per flower/10⁴
凤丹Fengdan	134.6±6.8 b	14.25±0.14 a	1.21±0.09 b	1.37±0.05 a	23.75±2.11 a	1 262.46±10.73 a
黄冠Huangguan	194.4±6.7 a	11.14±0.53 b	1.52±0.07 a	0.99±0.08 b	16.86±1.71 b	288.06±3.51 b

品种 Varieties	极轴 Polar axis length(P)/μm	赤道轴 Equator axis length(E)/μm	P/E	萌发沟 Germinal furrow/μm	外壁纹饰 Exine sculpture
凤丹Fengdan	48.62±1.13 a	23.07±0.45	2.10	42.12±1.41a	网状Reticular patterns
黄冠Huangguan	38.08±3.46 b	19.50±2.42	1.95	31.54±3.05b	网状Reticular patterns

2个牡丹切花品种核型、花药形态与花粉特性的比较

Comparative analysis of karyotype, anther morphology and pollen characteristics for two varieties of tree peony cut flower

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 49

相关文章 15

编辑推荐

Metrics

本文评价

培养时间 Incubation time/h	凤丹 Fengdan		黄冠 Huangguan
培养时间 Incubation time/h	25 ℃	35 ℃	25 ℃	35 ℃
0.5	18.66±1.37 c	15.33±3.84 b	0	0
1	24.83±1.58 b	36.23±0.71 a	0	0
2	46.61±2.41 a	33.97±1.12 a	0	0
3	48.85±4.38 a	33.38±1.49 a	0	0
4	51.85±4.21 a	34.68±0.89 a	0	0
5	50.93±4.61 a	34.89±1.59 a	0	0
6	52.24±4.32 a	31.95±3.37 a	0	0

类别Types	指标Index	凤丹Fengdan	黄冠Huangguan
有机物含量Organic substances/(mg·g^-1)	可溶性糖Soluble sugar	12.79±2.00	12.56±4.16
	淀粉Starch	7.82±1.28	9.00±1.28
	可溶性蛋白质Soluble protein	2.64±0.07 a	1.80±0.07 b
脂过氧化相关产物含量	MDA	57.74±2.20 a	41.58±6.37 b
Lipid peroxidation related products	H₂O₂	17.59±1.29 a	21.76±1.11 b
contents/(μmol·g^-1)	GSH	21.31±4.58	20.09±0.91
	AsA	241.85±5.29 a	146.75±8.00 b
酶活性Enzymes activities/(U·g^-1·min^-1)	SOD	134.27±3.84 a	71.62±0.36 b
	POD	1.55±0.12 b	3.37±0.24 a
	CAT	26.15±2.47	29.08±2.41

[1]	张美莹, 莫倩, 齐秀双, 佟宁宁, 孔凡, 刘政安, 吕长平, 彭丽平. 牡丹PoLPAT2基因的克隆及表达分析[J]. 浙江农业学报, 2025, 37(2): 321-328.
[2]	王苗苗, 秦嘉泽, 郭佳琪, 于天成. 杓唇石斛开花特性与繁育系统研究[J]. 浙江农业学报, 2025, 37(1): 103-114.
[3]	刘慧春, 许雯婷, 周江华, 张加强, 史小华, 朱开元. 基于牡丹涝害胁迫的转录组分析及SSR引物开发[J]. 浙江农业学报, 2024, 36(3): 544-558.
[4]	乔红雍, 袁涛, 赵信勇, 杨会岩. 不同株龄鲁菏红细根内生微生物群落变化特征[J]. 浙江农业学报, 2024, 36(1): 115-126.
[5]	刘芳芳, 陈红林, 刘峰, 许晓军, 黄福勇, 楼宝, 钱豪杰. 雌雄红螯螯虾染色体核型比较分析[J]. 浙江农业学报, 2023, 35(9): 2079-2089.
[6]	沈植国, 孙萌, 丁鑫, 程建明, 陈迪新. 不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响[J]. 浙江农业学报, 2021, 33(2): 278-287.
[7]	龙海梅, 张楠卿, 李宗艳, 王琴, 付超. 金盏菊根尖细胞染色体制片与核型分析[J]. 浙江农业学报, 2020, 32(1): 86-92.
[8]	原晓龙, 李云琴, 王毅. 滇牡丹中3个类查尔酮合成酶基因的克隆与表达[J]. 浙江农业学报, 2019, 31(9): 1478-1484.
[9]	张晓莹, 傅巧娟, 赵福康, 李春楠, 阮若昕. 大花蕙兰花粉贮藏与离体萌发研究[J]. 浙江农业学报, 2019, 31(9): 1502-1508.
[10]	于晓萌, 郝宏娟, 王丹, 王玲. CaCl₂处理对玉蝉花切花保鲜效果和生理特性的影响[J]. 浙江农业学报, 2017, 29(5): 773-781.
[11]	孙勃, 田玉肖, 夏雪, 李梦娇, 罗玲玲, 李琳, 陈清, 张芬, 汤浩茹. 叶用甜菜染色体制片条件优化及核型分析[J]. 浙江农业学报, 2016, 28(10): 1704-1710.
[12]	丁言，郭太君，孙晓刚，马赛. 凤丹牡丹种子浸提液对白菜种子萌发和幼苗生长的影响[J]. 浙江农业学报, 2015, 27(6): 961-.
[13]	胡吉燕，张星星，张敏，许薇薇，李金枝*. 6\\|BA和GA3配伍对玫瑰切花保鲜效果的影响[J]. 浙江农业学报, 2014, 26(5): 1223-.
[14]	陈琪;张贯臣;苏金乐. 洛阳牡丹花期预测模型构建与检验[J]. , 2013, 25(1): 0-78.
[15]	王凤兰;张昭其. 切花花瓣衰老机理研究进展[J]. , 2011, 23(5): 0-1068.