褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较

›› 2010, Vol. 22 ›› Issue (5): 653-659.

褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较

李娜¹,陈建明^2,*,张珏锋²,何月平²,陈列忠²

¹杭州师范大学生命与环境科学学院,浙江杭州310036; ²浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-25 发布日期:2010-09-25

Comparison for activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endosymbiotic strains of rice brown planthopper, Nilaparvata lugens Stal

LI Na;CHEN Jian-ming;*;ZHANG Jue-feng;HE Yue-ping;CHEN Lie-zhong

¹ College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China; ²Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-25 Published:2010-09-25

摘要/Abstract

摘要： 比较测定了褐飞虱共生菌抗吡虫啉菌株和敏感菌株3种解毒酶活性的变化。结果表明,褐飞虱共生菌不同抗感吡虫啉菌株3种解毒酶活性差异显著（P<0.05）,其中抗吡虫啉菌株的羧酸酯酶、多功能氧化酶活性显著高于敏感菌株,而谷胱甘肽-S-转移酶活性差异不显著。说明褐飞虱共生菌对吡虫啉抗药性的提高,与其体内羧酸酯酶、多功能氧化酶的活性增强有关。

关键词: 褐飞虱共生菌, 抗吡虫啉菌株, 敏感菌株, 解毒酶活性, 抗药性

Abstract: Activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endusymbiotic strains of rice brown planthopper, Nilaparvata lugens were compared. The results showed that resistant- and susceptible-imidacloprid strains had significant difference in activities of detoxifying enzymes, in which, activities of carboxylesterase, mixed function oxidase in resistant-imidacloprid strains were significantly higher than those in susceptible-imidacloprid strains, while activity of glutathione-S-transferase showed no obvious difference between resistant-and susceptible-imidacloprid strains. It was suggested that resistant increasing of N. lugens symbiotes to imidacloprid was related to enhencement of carboxylesterase, mixed function oxidase activities．

Key words: Nilaparvata lugens endosymbiotes, resistant-imidacloprid strain, susceptible-imidacloprid strain, activity of detoxifying enzymes, resistance

李娜;陈建明;*;张珏锋;何月平;陈列忠. 褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较[J]. , 2010, 22(5): 653-659.

LI Na;CHEN Jian-ming;*;ZHANG Jue-feng;HE Yue-ping;CHEN Lie-zhong. Comparison for activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endosymbiotic strains of rice brown planthopper, Nilaparvata lugens Stal[J]. , 2010, 22(5): 653-659.

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