浙江农业学报

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化学诱变甘薯新品种甬紫薯1号的选育

  

  1. (1 宁波市农业科学研究院,浙江 宁波 315040; 2宁海县农业技术推广总站,浙江 宁波 315600;3浙江省农业科学院 病毒学与生物技术研究所,浙江 杭州 310021)
  • 出版日期:2015-12-25 发布日期:2016-01-05

The chemical mutation breeding of a new type of sweet potato Yongzishu No.1

  1. (1 Ningbo Academy of Agricultural Sciences, Ningbo 315040,China;2 Agricultural Techniques Extension Station of Ninghai Country, Ningbo 315600,China;3 Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China )
  • Online:2015-12-25 Published:2016-01-05

摘要: 采用化学诱变育种手段选育紫薯新品种,分别用0,0.5%,1.0%,1.5%叠氮化钠(NaN3)对亲本澳大利亚Au1990sp紫甘薯胚性细胞团进行诱变处理6 h。结果表明,0.5% NaN3处理6 h效果最好,将05% NaN3处理6 h胚性细胞团进行再分化,共分化出植株125株,2003年移栽后获得99株系,当年入选14株系,秋天收获时其中有3株系高产,编号为CA06产量达到43.98 t·hm-2, CA11产量达到40.03 t·hm-2,CA17产量达到43.84 t·hm-2,对照组Au1990sp产量为4.95 t·hm-2。诱变后的株系在植株表型、特征、特性与亲本相比有很大的不同。对亲本Au1990sp用NaN3诱变后的株系进行AFLP 分析,发现有明显差异,对该品种进行12年的选育,命名为甬紫薯1号,2012年12月获得浙江省非农作物新品种的审定。

关键词: 甬紫薯1 号, 诱变育种, 品种特性

Abstract: In this study, we used chemical mutation breeding method to breed a new variety of purple sweet potato. We adopted 0, 0.5%, 1.0%, 1.5% of sodiumazide to treat with embryogenic cell masses for 6 hours by using parental Australian purple sweet potato of Au1990sp. The result showed that the treatment of 0.5% sodiumazide for 6 h was the best. After redifferentiation, the embryogenic callus in the treatment of 0.5% sodiumazide for 6 h were differentiated into 125 strains, and 99 strains were obtained after transplantation in 2003. Among the 14 strains selected in 2003, 3 strains obtained high yield, indicating 43.98 t·hm-2 of CA06, 40.03 t·hm-2 of CA11 and 43.84 t·hm-2 of CA17. The yield of the control group of Au1990sp was only 4.95 t·hm-2. Moreover, the mutated strains showed quite a lot of difference in phenotype, characteristics and features from parental strains. There were obvious differences between the parental strain of Au1990sp and the mutated strain by AFLP analysis. This new variety was named Yongzishu No.1 after 12 years breeding, which was approved to be a new non\|crop variety in December in 2012 in Zhejiang Province.

Key words: Yongzishu No. 1, mutation breeding, variety characteristic