浙江农业学报

• 作物科学 • 上一篇    下一篇

离子液体对玉米幼苗生长与生理生化特性的影响

  

  1. (河南师范大学 生命科学学院,河南 新乡 453007)
  • 出版日期:2015-12-25 发布日期:2016-01-05

Effects of ionic liquid on the growth, physiological and biochemical characteristics of maize seedlings

  1. (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)
  • Online:2015-12-25 Published:2016-01-05

摘要: 探究离子液体对作物的毒害作用,可以为研究离子液体对生态环境的影响提供依据。采用水培法研究了不同浓度(0,5,10,15 mg·L-1)的离子液体1\|己基\|3\|甲基咪唑溴化盐(1\|hexyl\|3\|methyl imidazolium bromide,[C6mim]Br)对玉米(囤玉118)幼苗生长以及叶片部分生理生化指标的影响。结果表明:在生长方面,当离子液体浓度≥10 mg·L-1时,玉米幼苗株高、根长均减少,差异达到显著水平;在生理生化方面,当离子液体浓度≥10 mg·L-1时,叶片中可溶性糖含量降低,达极显著水平;3个处理组的玉米幼苗叶片可溶性蛋白含量均极显著低于对照;当离子液体浓度≥10 mg·L-1时,过氧化物酶(POD)活性显著低于对照;当离子液体浓度≥15 mg·L-1时,超氧化物歧化酶(SOD)活性降低,超氧阴离子(O·-2)产生速率升高,均达到极显著水平。证明离子液体[C6mim]Br对主要农作物玉米具有一定的毒害作用,并且随着离子液体浓度的增加,毒害作用逐渐增大。

关键词: 离子液体, 玉米, 毒害, 可溶性蛋白, SOD, POD

Abstract: Investigating the toxic effects of ionic liquids on crops could provide basis for the effects of ionic liquid on ecological environment. Effects of 1\|hexyl\|3\|methyl imidazolium bromide ionic liquid(1\|hexyl\|3\|methyl imidazolium bromide, [C6mim]Br)on the growth, physiological and biochemical characteristics of maize seedlings(Tunyu 118) were evaluated by hydroponics with different concentrations of [C6mim]Br(0, 5, 10, 15 mg·L-1). The results showed that when [C6mim]Br concentration was greater than or equals to 10 mg·L-1, plant height and root length of maize seedling were decreased significantly(P<005), and the content of soluble sugar in the leaves was decreased significantly(P<001). Compared with the normal control, the contents of soluble protein in the leaves was decreased significantly whatever [C6mim]Br concentration was set in this study. The POD activities were significantly lower than that of the control when [C6mim]Br concentration was greater than or equals to 10 mg·L-1. When [C6mim]Br concentration was≥15 mg·L-1, the reducing of superoxide dismutase(SOD) activity and increasing of superoxide anion(O·-2) rate all reached significant level(P<001) compared with the control. It proved that ionic liquid [C6mim]Br was harmful to maize, and with the increase of ionic liquid concentration, the poison increased.

Key words: ionic liquid, maize, poison, soluble protein, SOD, POD