浙江农业学报

• 动物科学 • 上一篇    下一篇

家兔MYL12B基因的克隆及生物信息学分析

  

  1. (1 浙江师范大学 化学与生命科学学院,浙江 金华 321000;2 浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310000;3 扬州大学 动物科学与技术学院,江苏 扬州 225009)
  • 出版日期:2015-06-25 发布日期:2015-06-26

Clone and bioinformatics analysis of MYL12B gene in rabbit

  1. (1 College of Chemistry and life science, Zhejiang Normal University, Jinhua 321000, China; 2 Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)
  • Online:2015-06-25 Published:2015-06-26

摘要: 克隆家兔MYL12B基因的CDS序列并对其进行生物信息学分析。结果表明,家兔与其他动物MYL12B基因在CDS和氨基酸序列上的相似性较高。对家兔MYL12B蛋白的软件预测分析表明,该蛋白是一个无二硫键和信号肽的稳定的亲水性结构蛋白,含11个磷酸化位点和3个糖基化位点。研究结果可为该基因的进一步研究以及探明其作用机制和网络调控机理提供依据。

关键词: MYL12B基因, 相似性, 生物信息学分析

Abstract: The full\|length of rabbit MYL12B CDS was cloned and its bioinformatics was analyzed in this experiment. The results showed that there was high level of similarity in the CDS and amino acid sequences between rabbit and other animals, which manifested that MYL12B was a conservative protein. Bioinformatics analysis showed that the protein was a stable hydrophilic structural protein without disulfide bond and signal peptide, including 11 phosphorylation sites and 3 glycosylation sites. This study provides theoretical foundation for exploring the action mechanism and network regulation of MYL12B gene.

Key words: MYL12B gene, similarity, bioinformatics analysis