浙江农业学报

• 动物科学 • 上一篇    下一篇

不同物种Atrn基因编码区生物信息学分析

  

  1. (1河北农业大学 动物科技学院,河北 保定 071001;2河北科技师范学院 动物科技学院,河北 秦皇岛 066004)
  • 出版日期:2015-06-25 发布日期:2015-06-26

Bioinformatics analysis of coding regions of Atrn gene among different species

  1. (1 College of Animal science and Technology, Agricultural University of Hebei, Baoding 071001, China;2 College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinghuangdao 066004, China)
  • Online:2015-06-25 Published:2015-06-26

摘要: 采用生物信息学的方法分析了人、牛、野牦牛、小须鲸、水牛、狼、狨、狨鼠、白犀牛、星鼻鼹鼠、犰狳、小马岛猬、埃氏象鼩、猫、裸隐鼠、食蟹猴、猕猴、雪貂、小家鼠、长臂猿、中央狐蝠、褐家鼠、野猪、松鼠等24个物种Atrn基因编码区(CDS),并对该基因核苷酸多样性及氨基酸序列进行了分析和预测。结果表明,Atrn基因序列编码区的种内、种间存在丰富的遗传多样性,来自24个物种的61条基因序列中共检测到多态位点899个,生成31种单倍型。Atrn蛋白N端存在信号肽和跨膜区,属于定位于生物膜上的膜蛋白或分泌蛋白。Atrn蛋白为不稳定的亲水性蛋白,理论等电点为7.17,Atrn蛋白呈弱碱性,蛋白质二级结构主要结构元件是无规则卷曲和α\|螺旋。

关键词: 物种, Atrn基因, 生物信息学分析

Abstract: The coding sequence (CDS), nucleotide diversity and amino acid sequence of Atrn gene from Homo sapiens, Bos Taurus, Bos mutus, Balaenoptera acutorostrata, Bubalus bubalis, Canis lupus, Callithrix jacchus, Chinchilla lanigera, Ceratotherium simum, Condylura cristata, Dasypus novemcinctus, Echinops telfairi, Elephantulus edwardii, Felis catus, Heterocephalus glaber, Macaca fascicularis, Macaca mulatta, Mustela putorius furo, Mus musculus, Nomascus leucogenys, Pteropus alecto, Rattus norvegicus, Sus scrofa and Spermophilus tridecemlineatus were analyzed using the method of bioinformatics. The results showed that the Atrn gene CDS had abundant genetic diversity between intraspecific and interspecific species, and a total of 899 polymorphic sites were detected from 61 sequences of 24 species which generated 31 haplotypes. The results also showed that Atrn has trans\|membrane domain and signal peptide in N\|terminal and belonged to membrane protein or secretion protein which were located on the biofilm. The protein was hydrophilic, instable and alkalescent, with an isoelectric point of 7.17. The main structural element of predicted secondary structure of Atrn were random coil and alpha helix.

Key words: species, Atrn gene, bioinformatics analysis