复方柴芩颗粒对鸭黄曲霉毒素慢性中毒CYP450酶活性的影响

浙江农业学报

复方柴芩颗粒对鸭黄曲霉毒素慢性中毒CYP450酶活性的影响

（四川农业大学动物医学院，四川成都 611130）

出版日期:2016-01-25 发布日期:2016-01-19

Effects of compound Chai Qin particles on enzymic activity of CYP450 in duck with aflatoxicosis

（College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 611130， China）

Online:2016-01-25 Published:2016-01-19

摘要/Abstract

摘要： 建立肉鸭aflatoxin B1（AFB1）慢性中毒模型，设立正常组、模型组、亚硒酸钠组、复方柴芩颗粒（中药）高、中、低剂量组。造模后第7，14和21天处死肉鸭取肝，采用酶联免疫吸附测定法（ELISA）检测肝标本中CYP3A4，CYP1A2和CYP2E1活性。结果表明，与模型组相比，中药高、中、低剂量组用药7和14 d后肝脏微粒体细胞色素P450酶系CYP3A4，CYP1A2和CYP2E1活性显著降低；用药21 d后CYP3A4，CYP1A2和CYP2E1活性升高，但与模型组相比差异不明显。说明前期复方柴芩颗粒通过抑制CYP3A4，CYP1A2和CYP2E1活性，减少AFB1前体物质代谢激活，降低其化学性肝脏损伤达到保护肝脏的作用。而用药后21 d CYP450活性升高的原因可能是AFB1蓄积较多，需要更多的CYP450代谢AFB1。

关键词: 细胞色素CYP1A2, 细胞色素CYP3A4, 细胞色素CYP2E1, 复方柴芩颗粒, 黄曲霉毒素B1

Abstract: An aflatoxin B1 （AFB1） poisoning model was firstly established in the present study. Ducks were divided into control group， Na2SeO3 group， model group， low/medium/high\|dose compound Chai Qin particles group. 7， 14， 21 d after treatment， liver tissues of ducks were taken to detect of the activities of CYP3A4， CYP1A2 and CYP2E1. Compared with model group， activities of CYP3A4， CYP1A2 and CYP2E1 in liver after 7， 14 d decreased significantly in three compound Chai Qin particles groups regardless of doses. Then， the activities of CYP3A4， CYP1A2 and CYP2E1 increased in these three groups after 21 d. But， there were no significant differences in the activities of CYP3A4， CYP1A2 and CYP2E1 among model group and compound Chai Qin particles groups. It was suggested that compound Chai Qin particles could effectively decrease activities of CYP1A2， CYP2E1 and CYP3A4 in the early treatment of aflatoxicosis by decreasing the activation of AFB1， which could reduce the chemical liver injury， and the increasing activities of CYP450 after 21 d treatment with compound Chai Qin particles might be caused by the increasing demand of CYP450 to metabolize AFB1.

Key words: cytochrome CYP1A2, cytochrome CYP3A4, cytochrome CYP2E1, compound Chai Qin particles, aflatoxin B1

李杨，高祝，荣茜，杨晓敏，张睿，李英伦*. 复方柴芩颗粒对鸭黄曲霉毒素慢性中毒CYP450酶活性的影响[J]. 浙江农业学报.

LI Yang， GAO Zhu， RONG Qian， YANG Xiao\|min， ZHANG Rui， LI Ying\|lun*. Effects of compound Chai Qin particles on enzymic activity of CYP450 in duck with aflatoxicosis[J]. .

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