浙江省不同地理种群红火蚁主要表皮碳氢化合物的差异性分析

doi:10.3969/j.issn.1004-1524.20220808

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (7): 1672-1679.DOI: 10.3969/j.issn.1004-1524.20220808

浙江省不同地理种群红火蚁主要表皮碳氢化合物的差异性分析

柳跃泽¹^,²(), 黄俊²^,^*(), 张娟³, 李晓维², 章金明², 张治军², 吕要斌¹^,²^,^*()

1.中国计量大学生命科学学院,浙江杭州 310018
2.浙江省农业科学院植物保护与微生物研究所,农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021
3.浙江省园林植物与花卉研究所,浙江杭州 311122

收稿日期:2022-06-01 出版日期:2023-07-25 发布日期:2023-08-17
作者简介:柳跃泽(1995—),男,河北辛集人,硕士研究生,研究方向为药理与毒理。E-mail: Liu15081883492@163.com
通讯作者: *黄俊,E-mail: junhuang1981@126.com;
吕要斌,E-mail: luybcn@163.com
基金资助:
国家重点研发计划(2021YFD1000500);浙江省公益技术应用研究计划(LGN21C140005);浙江省“三农九方”科技协作项目(2022SNJF075);杭州市农业与社会发展科研项目(20201203B161)

Difference analysis of main cuticular hydrocarbons of red imported fire ant (Solenopsis invicta Buren) from different geographical populations in Zhejiang Province, China

LIU Yueze¹^,²(), HUANG Jun²^,^*(), ZHANG Juan³, LI Xiaowei², ZHANG Jinming², ZHANG Zhijun², LYU Yaobin¹^,²^,^*()

1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Zhejiang Institute of Landscape Plants and Flowers, Hangzhou 311122, China

Received:2022-06-01 Online:2023-07-25 Published:2023-08-17
Contact: HUANG Jun,LYU Yaobin

摘要/Abstract

摘要：

红火蚁(Solenopsis invicta Buren)是一种真社会性昆虫,是世界上最具危险的100种入侵有害生物之一。红火蚁表皮碳氢化合物(cuticular hydrocarbons, CHCs)是其同巢识别的信号物质,对其种群的稳定具有重要作用。目前,已有关于红火蚁主要体表碳氢化合物在近缘种间和不同虫态及品级间的差异比较,但是不同地理种群和红火蚁身体不同部位CHCs组分及其相对比例的差异性却鲜见报道。本文利用气相色谱质谱联用技术(GC-MS)对浙江省温州、丽水及义乌3个不同地理种群红火蚁老龄工蚁的CHCs进行比较分析。结果表明,红火蚁头部CHCs含量最高,达到50%以上,其次是胸部和腹部,而且在其身体各部位之间显著差异,CHCs含量在不同地理种群之间无显著差异。进一步分析CHCs主要组分的相对比例发现,该数值在红火蚁头部表现较为规律,5种主要CHCs中的3-甲基二十七烷和3,9-二甲基二十七烷在不同地理种群之间较为波动,而正二十七烷、13-甲基二十七烷和13,15-二甲基二十七烷在温州和丽水种群之间较为稳定。本研究能为今后揭示遗传及环境因素对红火蚁巢间个体CHCs主要组分的调节机制提供重要参考依据。

关键词: 红火蚁, 地理种群, 表皮碳氢化合物, 同巢识别

Abstract:

Red imported fire ant (Solenopsis invicta Buren) is an eusocial insect, and is one of the 100 most dangerous invasive pests in the world. Cuticular hydrocarbons (CHCs) are important signal substances for nestmate recognition of S. invicta and play an important role in population stability. Recently, the differences of main CHCs of S. invicta among related species, different insect stages and various castes have been compared, but the differences of CHCs components and their relative proportions in different geographical populations and different body parts of S. invicta have been rarely reported. Here, the CHCs of 3 different geographical populations (Wenzhou, Lishui and Yiwu) of S. invicta were comparatively analyzed by gas chromatography-mass spectrometry (GC-MS). Further analysis of the relative proportion of the main components of CHCs showed that this value was relatively regular in the head of S. invicta. 3-methyl heptadecane and 3,9-dimethyl heptadecane in the 5 main CHCs fluctuated among different geographical populations, while n-heptadecane, 13-methyl heptadecane and 13,15-dimethyl heptadecane were relatively stable between Wenzhou and Lishui populations. This study can provide important reference for revealing the regulation mechanism of genetic and environmental factors on CHCs components of red imported fire ants.

Key words: Solenopsis invicta, geographical population, cuticular hydrocarbons, nestmate recognition

中图分类号:

S433

柳跃泽, 黄俊, 张娟, 李晓维, 章金明, 张治军, 吕要斌. 浙江省不同地理种群红火蚁主要表皮碳氢化合物的差异性分析[J]. 浙江农业学报, 2023, 35(7): 1672-1679.

LIU Yueze, HUANG Jun, ZHANG Juan, LI Xiaowei, ZHANG Jinming, ZHANG Zhijun, LYU Yaobin. Difference analysis of main cuticular hydrocarbons of red imported fire ant (Solenopsis invicta Buren) from different geographical populations in Zhejiang Province, China[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1672-1679.

图/表 3

图1 红火蚁5种主要表皮碳氢化合物总离子色谱图图中1~5序号分别表示正二十七烷、13-甲基二十七烷、13,15-二甲基二十七烷、3-甲基二十七烷和3,9-二甲基二十七烷。

Fig.1 Total ion flow diagram of 5 major cuticular hydrocarbons of Solenopsis Invicta The names of cuticular hydrocarbons labeled as 1-5 are n-heptacosane, 13-methylheptacosane, 13,15-dimethylheptacosane, 3-methylheptacosane, and 3,9-dimethylheptacosane, respectively.

图2 不同地理种群红火蚁各部位表皮碳氢化合物的总含量差异比较同一种群标注不同小写字母表示差异显著(P<0.05)。

Fig.2 Comparison of the total content of cuticular hydrocarbons of different geographical populations of Solenopsis Invicta Different lowercase letters on the same population indicated significant difference (P<0.05) from each other.

图3 不同地理种群红火蚁各部位主要表皮碳氢化合物的相对比例图中①~⑤分别表示图1中表述的5种主要表皮碳氢化合物。ns表示两者间无显著差异(P>0.05);*表示两者间存在显著差异(P<0.05),**表示两者间存在极显著差异(P<0.01)。WZ,温州种群;LS,丽水种群;YW,义乌种群。

Fig.3 The relative proportions of main cuticular hydrocarbons in different body parts of different geographical populations of Solenopsis Invicta The 5 main cuticular hydrocarbons described in Fig. 1 are represented by ① to ⑤ respectively. ns indicates no significant difference from each other (P>0.05); * and ** indicate a significant difference from each other at P<0.05 and P<0.01, respectively.WZ, Wenzhou population; LS, Lishui population; YW, Yiwu population.

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浙江省不同地理种群红火蚁主要表皮碳氢化合物的差异性分析

Difference analysis of main cuticular hydrocarbons of red imported fire ant (Solenopsis invicta Buren) from different geographical populations in Zhejiang Province, China

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