关键词: 灰葡萄孢, T\|DNA插入突变体, CFW敏感性, 细胞壁缺陷

Abstract: 234 T-DNA insertion mutants of Botrytis cinerea were screened by using Calcofluor White, and three sensitive mutants(B-117, B-169, D-9)and one resistant mutant(B-62)were obtained. In the test with isotonic PDA plates containing 1.2 mol·L-1 sorbitol, the growth of D-9 was not affected or rescued, while the growth of wild type was suppressed. Also, the growth of wild type was suppressed by 0.6 mol·L-1 NaCl but D-9 was not affected. No obvious difference was found between wild type and D-9 in the SDS medium. The spores of D-9 appeared swollen, its hyphae were thicker, and the length increased without bending,which was obviously different from that of wild type in the spore germination assay. In the experiment of tomato infection, the virulence of D-9 mutant was significantly reduced, almost no infection phenomenon can be observed in the period of six days. These results indicated that D-9 mutant was probably related to cell wall defect and pathogenicity.

Key words: Botrytis cinerea, T-DNA insertion mutants, sensitive to CFW, cell wall defect