浙江地区不同寄主来源的炭疽菌ITS序列PCRRFLP多态性分析

›› 2012, Vol. 24 ›› Issue (1): 0-65.

• 植物保护 •

浙江地区不同寄主来源的炭疽菌ITS序列PCRRFLP多态性分析

刘诗胤1，2，邱海萍1，姜华1，张震1，柴荣耀1，毛雪琴1，王教瑜1，杜新法1，王艳丽1，*，孙国昌1，*

1浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地浙江省农业科学院植物保护与微生物研究所，浙江杭州 310021；2杭州师范大学生命与环境科学学院，浙江杭州 310036

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-24 发布日期:2012-01-24

Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP

LIU Shiyin;QIU Haiping;JIANG Hua;ZHANG Zhen;CHAI Rongyao;MAO Xueqin;WANG Jiaoyu;DU Xinfa;WANG Yanli;*;SUN Guochang;*

1State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control，Institute of Plant Protection and Microbiology，Zhejiang Academy of Agricultural Sciences， Hangzhou 310021，China；2College of Life and Environment Sciences，Hangzhou Normal University，Hangzhou 310036，China

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-24 Published:2012-01-24

摘要/Abstract

摘要：

摘要：应用核糖体DNA (ribosomal DNA，rDNA)的ITS(internal transcribed spacer， ITS) 区的RFLP序列的多态性，分析32个不同寄主来源的炭疽菌株的遗传多样性。利用ITS1及 ITS4通用引物对扩增各菌株的rDNA的内转录间隔区(ITS158SITS2)获得长约600 bp片段。ITSRFLP共迁带UPGMA聚类分析的结果表明所有来自不同寄主的炭疽菌菌株(含福建及云南菌株)都以较高的相似系数(>0.60)聚为一类，具有较近的亲缘关系；同时试验中所有酶切位点的综合Gst系数达到0.82，说明不同菌株间的ITS具有丰富的多态性。

关键词: 炭疽菌, rDNA, 内转录间隔区, PCRRELP, 亲缘关系

Abstract: Colletotrichum spp. is the most common fungi in Zhejiang Province as well as the most important plant pathogens. There are so many kinds of species that it is important to find a way to identify and classify these fungi. Genetic diversity among 32 strains belonging to Colletotrichum spp. was assessed by RFLP analysis on the basis of rDNA ITS region. The PCR amplified ITS region was about 600 bp in length in all the tested strains and RFLP patterns of ITS products digested with different endonucleases (AluⅠ， BsuRⅠ， Hha Ⅰ， HpaⅡ and TaqⅠ) were analyzed. The UPGMA phenogram constructed with the pool of the ITS PCRRFLP data revealed that all the isolates were clustered on one big group when the value of genetic similarity was 0.6. The Gst parameter of all the ITS PCRRFLP polymorphic bands was 0.82 which indicated there were high diversity in the ITS region of all the isolates．

Key words: Colletotrichum spp., rDNA, ITS, PCRRFLP, genetic relationships

刘诗胤;邱海萍;姜华;张震;柴荣耀;毛雪琴;王教瑜;杜新法;王艳丽;*;孙国昌;* . 浙江地区不同寄主来源的炭疽菌ITS序列PCRRFLP多态性分析[J]. , 2012, 24(1): 0-65.

LIU Shiyin;QIU Haiping;JIANG Hua;ZHANG Zhen;CHAI Rongyao;MAO Xueqin;WANG Jiaoyu;DU Xinfa;WANG Yanli;*;SUN Guochang;*. Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP [J]. , 2012, 24(1): 0-65.

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浙江地区不同寄主来源的炭疽菌ITS序列PCRRFLP多态性分析

Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCRRFLP

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