›› 2012, Vol. 24 ›› Issue (3): 0-444.

• 论文 •    

外源NO对非洲菊切花保鲜效果及作用机制


杜兴翠1,任雪1, 杨尧1,章四庆1,郭巧会1,赖齐贤2,*
  

  1. 1浙江农林大学 园林学院,浙江 临安 311300;2浙江农林大学 农业与食品科学学院,浙江 临安 311300
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-24 发布日期:2012-05-24

Effects of exogenous NO on preservation in cut gerbera and its mechanism

DU Xing-cui;REN Xue;YANG Yao;ZHANG Si-qing;GUO Qiao-hui;LAI Qi-xian;*   

  1. 1School of Landscape Architecture, Zhejiang A & F University, Linan 311300, China;2School of Agriculture and Food Science, Zhejiang A & F University, Linan 311300, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-24 Published:2012-05-24

摘要: 以硝普钠(SNP)作为外源NO的供体,分别采用0,10,100,500 μmol·L-1 浓度的SNP溶液作为非洲菊‘阳光海岸’的瓶插液,观察NO对非洲菊鲜切花的保鲜效果。同时,测定了花瓣超氧化物歧化酶(SOD),过氧化物酶(POD)、过氧化氢酶(CAT)活性的变化以及H2O2、丙二醛(MDA)、可溶性蛋白含量的变化。结果显示,100 μmol·L-1浓度处理的非洲菊切花瓶插寿命最长,比对照延长了31.4%;外源NO提高了花瓣SOD,POD,CAT等保护酶的活性,也提高了可溶性蛋白质的含量,而H2O2,MDA含量比对照降低,从而延缓了非洲菊切花花瓣的衰老。

关键词: 非洲菊, 硝普钠, 保鲜, 抗氧化酶, 衰老

Abstract: Using SNP as a donor of exogenous NO, cut gerbera variety Yangguanghaian was treated by vase solution of 0, 10, 100, 500 μmol·L-1 SNP. Meanwhile, the activities of antixoidase SOD, POD, CAT and the content of H2O2, MDA and soluble protein were investigated in petals. It showed that the vase life of cut gerbera was extended by 31.4% with the 100 μmol·L-1 SNP solution. The activities of SOD, POD, CAT and the concentration of soluble protein in petals increased. Meanwhile, the concentrations of H2O2, MDA were reduced by exogenous NO. In summary, the senescence of cut gerbera petals was delayed by exogenous NO in this study.

Key words: gerbera, SNP, preservation, antioxidase, senescence