一株抗大黄鱼细菌性病原菌的活性海洋真菌的鉴定

›› 2012, Vol. 24 ›› Issue (5): 0-807.

• 论文 •

一株抗大黄鱼细菌性病原菌的活性海洋真菌的鉴定

刘辉辉1,李书平2,赵倩1,赵淑江1,*

1温州医学院环境与公共卫生学院,浙江温州 325035；2郑州华信学院药学系,河南郑州 451150

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-24 发布日期:2012-09-24

Identification of marine fungal strain NJ0104 with the anti-microbial activities of their metabolites to bacterial pathogen of Pseudosciaena

LIU Hui-hui;LI Shu-ping;ZHAO Qian;ZHAO Shu-jiang;*

1School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou 325035, China；2Department of Pharmacy, Zhengzhou Huaxin Xueyuan, Zhengzhou 451150, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-09-24 Published:2012-09-24

摘要/Abstract

摘要： 为了研究从浙江省温州市南麂岛近海海域海洋沉积物样品中分离得到的一株具有抗大黄鱼病原菌活性的真菌NJ0104的分类地位,在标准培养条件下,采用传统形态鉴定方法,结合ITS-5.8S rDNA片段PCR扩增和序列测定,建立系统发育树。形态学特征显示菌丝呈树枝状分支,分支较多,无核,有隔膜,瓶体式产孢；ITS序列进行测序全长为1 027 bp。结合形态学特征和GenBank中核酸数据比对分析。结果表明,菌株NJ0104与节菱孢属亲缘关系较近,具有98%以上的相似性,因此将其鉴定为节菱孢属(Arthrinium sp.)。

关键词: 抗大黄鱼病原菌, 抑菌活性, 形态学, ITS序列, NJ系统发育树

Abstract: A marine fungal strain NJ0104, isolated from marine sediments near Nanji island sea, Wenzhou, Zhejiang, could produce active metabolites against bacterial pathogen of Pseudosciaena. To identify this strain, morphological and ITS-58S rDNA regions of the strain was amplified through PCR reaction and sequenced. Mycelium showed a polybranch morphology, there was no nucleus, but septum were present in the mycelium and produced conidiospores. The length of ITS sequence was 1027 bp. The results of morphological observation and genetic relationship of strain NJ0104 was close to Arthinium and the similarity was more than 98%. Based upon the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp．

Key words: anti-microbial pathogen of Pseudosciaena, antibacterial activity, morphological characteristics, ITS sequences, NJ phylogeny tree

刘辉辉;李书平;赵倩;赵淑江;*. 一株抗大黄鱼细菌性病原菌的活性海洋真菌的鉴定[J]. , 2012, 24(5): 0-807.

LIU Hui-hui;LI Shu-ping;ZHAO Qian;ZHAO Shu-jiang;*. Identification of marine fungal strain NJ0104 with the anti-microbial activities of their metabolites to bacterial pathogen of Pseudosciaena[J]. , 2012, 24(5): 0-807.

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一株抗大黄鱼细菌性病原菌的活性海洋真菌的鉴定

Identification of marine fungal strain NJ0104 with the anti-microbial activities of their metabolites to bacterial pathogen of Pseudosciaena

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