关键词: 硝基呋喃代谢物, 胶体金法, 水产品, 快速检测

Abstract: The fast methods of the derivatization of nitrofuran metabolites（AOZ, SEM, AMOZ, AHD） in aquatic products were investigated. The results showed that the optimal derivation condition was adding 4\|carbonyl\|benzaldehyde （4\|CBA） or 2\|nitrobenzaldehyde （2\|NBA） solution， then insulating in 60℃ constant temperature water bath for 60 min in acid medium. By comparing and selecting the clean\|up， extraction， and purification methods of the samples，the colloidal gold rapid test methods for the determination of nitrofuran metabolites in aquatic products had been developed and the colloidal gold rapid test kit had been successfully produced. Their lowest detection limits for AOZ， SEM， AMOZ and AHD were 05， 1. 0， 10 and 10 μg·kg-1 respectively， and the shortest test time for one sample was 90 min. These test results were confirmed by the methods of liquid chromatography\|tandem mass spectrometry and the coincidence rate of two methods was 98%. The colloidal gold rapid test methods were fast， easy to handle， inexpensive and of great application value for rapid detection．

Key words: nitrofuran metabolite, colloidal gold method, aquatic product, rapid test