牛肉及其制品中掺入鸡肉、鸭肉和猪肉的多重数字PCR快速检测方法研究

doi:10.3969/j.issn.1004-1524.2017.06.20

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 994-1000.DOI: 10.3969/j.issn.1004-1524.2017.06.20

牛肉及其制品中掺入鸡肉、鸭肉和猪肉的多重数字PCR快速检测方法研究

杨华, 汪小福, 肖英平, 魏巍, 徐俊锋

浙江省农业科学院农产品质量标准研究所, 浙江杭州 310021

收稿日期:2017-04-24 出版日期:2017-06-20 发布日期:2017-09-07
作者简介:杨华(1972—),男,浙江绍兴人,硕士,高级畜牧师,主要从事畜产品质量安全研究。E-mail: yanghua806@hotmail.com
基金资助:
浙江省自然科学基金(LZ15C170001)

Rapid detection of chicken,duck and pork blending in beef products by multiple digital PCR

YANG Hua, WANG Xiaofu, XIAO Yingping, WEI Wei, XU Junfeng

Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-04-24 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 利用微滴式数字PCR,建立针对牛、猪、鸡和鸭肉的多重数字PCR检测技术。结果表明,所选择的4个物种的引物和探针特异性良好,能够快速对样品中是否存在这4种肉类成分做出快速判断。数字PCR分析中,FAM和HEX两个通道的荧光信号能很好地区分,在对9个实际样品的检测中,所在样品的微滴生成数都在10 000以上,满足数字PCR中泊松公式分析的要求,同时9个样品的检测结果与已知信息完全相符。

关键词: 数字PCR, 肉源性成分检测, 多重检测, 快速检测

Abstract: In this study,a quickly detective method,using droplet digital PCR,for detection of beef,chicken,duck and pork was established. The results showed that the selected primers and probes for the four species had good specificity,and could quickly make out these four kinds of mean component in tested samples. In all of the digital PCR analysis, the fluorescence signals of FAM and HEX can be well distinguished. For detection of 9 samples,the numbers of droplets generated in the multiple digital PCR were all above 10 000, which could meet the requirements for the poisson formula analysis in digital PCR. meanwhile,the test results of the 9 samples using multiple digital were consistent with the known information of these samples.

Key words: digital PCR, meat components detection, multiple detection, quickly detection

中图分类号:

Q-331

杨华, 汪小福, 肖英平, 魏巍, 徐俊锋. 牛肉及其制品中掺入鸡肉、鸭肉和猪肉的多重数字PCR快速检测方法研究[J]. 浙江农业学报, 2017, 29(6): 994-1000.

YANG Hua, WANG Xiaofu, XIAO Yingping, WEI Wei, XU Junfeng. Rapid detection of chicken,duck and pork blending in beef products by multiple digital PCR[J]. , 2017, 29(6): 994-1000.

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牛肉及其制品中掺入鸡肉、鸭肉和猪肉的多重数字PCR快速检测方法研究

Rapid detection of chicken,duck and pork blending in beef products by multiple digital PCR

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