苯甲酰矢车菊素-3-葡萄糖苷的酶法合成及结构表征

doi:10.3969/j.issn.1004-1524.2017.06.21

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 1001-1008.DOI: 10.3969/j.issn.1004-1524.2017.06.21

苯甲酰矢车菊素-3-葡萄糖苷的酶法合成及结构表征

闫征, 李春阳, 黄午阳, 张丽霞^*

江苏省农业科学院农产品加工研究所,江苏南京 210014

收稿日期:2016-12-26 出版日期:2017-06-20 发布日期:2017-09-07
通讯作者: 张丽霞,E-mail: zlx5885@163.com
作者简介:闫征(1978—),男,黑龙江齐齐哈尔人,博士,助理研究员,研究方向为食品生物技术。E-mail: yz3737@sina.com
基金资助:
国家自然科学基金(31401489)

Synthesis technology and benzoylation cyanidin-3-glucoside catalyzed by lipase and structural characterization

YAN Zheng, LI Chunyang, HUANG Wuyang, ZHANG Lixia^*

Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2016-12-26 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 采用响应面分析确定脂肪酶催化合成酰基化矢车菊素-3-葡萄糖苷的最佳工艺,并通过液质联用和核磁共振对产物进行鉴定。结果显示,以酰基化反应转化率为指标的最佳工艺条件为:反应温度41 ℃,真空度-90 kPa,酶浓度43 mg·mL^-1,矢车菊素-3-葡萄糖苷浓度10 mg·mL^-1,苯甲酸甲酯浓度0.4 g·mL^-1,反应时间24 h,最终转化率为90.92%。矢车菊素-3-葡萄糖苷的酰基化产物经液质联用和核磁共振鉴定为矢车菊素-3-(6-苯甲酰)葡萄糖苷。

关键词: 矢车菊素-3-葡萄糖苷, 苯甲酰化, 脂肪酶

Abstract: The synthetic process of acylated cyanidin-3-glucoside catalyzed by enzyme was optimized through single factor experiment and response surface analysis according to the degree of conversion, and the product was identified by HPLC-MS and NMR. The optimum conditions were as follows: 41 ℃,-90 kPa, 43 mg·mL^-1 enzyme applied, concentration of cyanidin-3-glucoside 10 mg·mL^-1, concentration of methyl benzoate 0.4 g·mL^-1, reaction time 24 h. The structures of acylated cyanidin-3-glucoside products were identified as 3-(6″-benzoyl)-glucoside.

Key words: cyanidin-3-glucoside, benzoylation, lipase

中图分类号:

TQ464.3

闫征, 李春阳, 黄午阳, 张丽霞. 苯甲酰矢车菊素-3-葡萄糖苷的酶法合成及结构表征[J]. 浙江农业学报, 2017, 29(6): 1001-1008.

YAN Zheng, LI Chunyang, HUANG Wuyang, ZHANG Lixia. Synthesis technology and benzoylation cyanidin-3-glucoside catalyzed by lipase and structural characterization[J]. , 2017, 29(6): 1001-1008.

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苯甲酰矢车菊素-3-葡萄糖苷的酶法合成及结构表征

Synthesis technology and benzoylation cyanidin-3-glucoside catalyzed by lipase and structural characterization

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