浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 44-50.DOI: 10.3969/j.issn.1004-1524.2017.01.07

• 动物科学 • 上一篇    下一篇

病死猪堆肥高效油脂降解菌的筛选及堆肥效果研究

肖翰, 刘标, 尹红梅, 许隽, 杜东霞, 贺月林*   

  1. 湖南省微生物研究院,湖南 长沙 410009
  • 收稿日期:2016-08-17 出版日期:2017-01-15 发布日期:2017-02-23
  • 通讯作者: 贺月林,E-mail:hyl200866@yeah.net
  • 作者简介:肖翰(1974—),男,湖南涟源人,工程师,从事环境微生物研究。E-mail:1227204689@qq.com
  • 基金资助:
    湖南省科技厅农业支撑计划(2015NK3057); 湖南省生猪产业技术体系生猪产业规模养殖与环境控制岗位项目

Isolation and identification of oil degradation bacteria for efficient dead-pig composting

XIAO Han, LIU Biao, YIN Hongmei, XU Jun, DU Dongxia, HE Yuelin*   

  1. Hunan Microbiology Institute, Changsha 410009, China
  • Received:2016-08-17 Online:2017-01-15 Published:2017-02-23

摘要: 为加快病死猪堆肥过程中油脂的降解,从受油脂污染的样品中分离、筛选高效油脂降解菌,并将其接种至堆肥中进行堆肥效果验证。共分离得到6株具有油脂降解能力的菌株,其中菌株D-4降解能力最强。在初始筛选条件下,该菌对油脂的降解率达57.21%,在发酵48~54 h时达到产脂肪酶的高峰。经形态特征、生理生化特征、16S rDNA序列系统发育分析,将其鉴定为黏质沙雷氏菌(Serratia marcescens)。D-4菌株不同添加量的堆肥效果表明,接种D-4菌各组堆肥的油脂含量均显著(P<0.05)低于各时期对照组中的油脂含量。结果表明,筛选获得的菌株D-4为高效油脂降解菌株,可以用于病死猪腐熟堆肥,加快病死猪中油脂的降解。

关键词: 油脂降解菌, 脂肪酶, 沙雷氏菌属, 堆肥效果

Abstract: To accelerate the oil degradation of dead-pig, microorganisms able to degrade lipids efficiently were screened and isolated from oil-polluted samples. The isolated strains were mixed and inoculated in composting of dead-pigs and wheat bran with different doses to test its effects on oil degradation. Six oil-degrading strains were isolated, and among them strain D-4 showed the highest degradation ability, with the degradation rate up to 57.21%. The lipase production of strain D-4 reached a peak at 48~54 h of fermentation. Based on morphological, physiological and biochemical characteristics, and phylogenic analysis of 16S rDNA sequences, strain D-4 was identified as Serratia marcescens. At the end of composting, the oil contents of all inoculation groups with strain D-4 were significantly (P<0.05)lower than that of the control group. It was indicated that sufficient amount inoculation of D-4 could effectively accelerate lipid degradation of dead-pig by composting.

Key words: oil-degrading strain, lipase, Serratia sp., composting effect

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