浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 51-57.DOI: 10.3969/j.issn.1004-1524.2017.01.08

• 动物科学 • 上一篇    下一篇

鹅线粒体COⅠ条形码及上游tRNA序列测定分析

张扬1, 王美青1, 穆春宇1, 姚文成1, 杨耀宗1, 卞友庆2, 陈国宏1, *   

  1. 1.扬州大学 动物科学与技术学院,江苏 扬州 225009;
    2.江苏现代畜牧科技园,江苏 泰州225300
  • 收稿日期:2016-05-30 出版日期:2017-01-15 发布日期:2017-02-23
  • 通讯作者: 陈国宏, E-mail:ghchen@yzu.edu.cn
  • 作者简介:张扬(1986—),男,山东临沂人,讲师,博士,主要从事动物遗传育种与繁殖研究。E-mail:zyang@yzu.edu.cn
  • 基金资助:
    现代农业产业技术体系(CARS-43-3); 江苏省农业科技自主创新资金项目[CX(13)2034]

Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination

ZHANG Yang1, WANG Meiqing1, MU Chunyu1, YAO Wencheng1, YANG Yaozong1, BIAN Youqing2, CHEN Guohong1, *   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Modern Animal Husbandry Science and Technology Park, Taizhou 225300, China
  • Received:2016-05-30 Online:2017-01-15 Published:2017-02-23

摘要: 为探索有效识别家禽(鹅)品种的线粒体DNA条形码,以对家禽品种或禽源产品进行鉴别,文章以鸿雁、灰雁、6个地方鹅种(中国、欧洲各3个)为试验群体,利用直接测序技术对所有个体的线粒体细胞色素C氧化酶亚基Ⅰ基因(COⅠ)进行测序,并分析多态性以及分子系统进化关系,同时对该区域上游tRNA的序列进行分析。结果表明,COⅠ该段序列(1 551 bp)共存在17个突变位点,形成20种单倍型,其中18种为各群体特有,可以作为群体鉴定的依据;除卡洛斯鹅外,其余7个群体中均发现有特异变异位点。基于Kiumura双参数距离所构建的单倍型NJ树能够充分反映不同群体的进化的亲缘关系。进一步分析发现了鸿雁线粒体序列有22种tRNA基因,均为标准4个恒定臂三叶草结构,其中tRNA-Ser和tRNA-Leu各有2个,其余仅有1个。选取的COⅠ该段序列可以作为部分鹅品种鉴定的依据。

关键词: 鹅, 基因条形码, tRNA, 序列分析

Abstract: This study was conducted to find the effective and accurate identification of the DNA bar code in poultry breed (goose), in order to identify poultry breed or the products of goose. In this study, Anser cygnoides, Anser anser, 3 breeds of local Chinese goose and 3 breeds of European goose were used. Gene sequence based on the mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ) was conducted by DNA sequence, and the polymorphism, the relationship of molecular system evolution and the upstream region of the tRNA sequences on goose were also determined. The results showed that: 1) There were 17 mutation sites and 20 haplotypes in the sequence of COⅠ(1 551 bp). 2) Eighteen haplotypes were specific to each species, which could be used as the basis for identification. 3) Seven breeds had specific mutation sites except Carlos. Based on this sequence, the phylogenetic tree constructed by the Kiumura double parameter distance can fully reflect the relationship between the classification and evolution of different geese. 4) Sequence analysis found that Anser cygnoides had 22 kinds of mitochondrial tRNA gene, which were the standard of four constant arm of cloverleaf secondary structure, including 2 tRNA-Ser, 2 tRNA-Leu and the rest were 1. This sequence of COⅠ could be used as the basis for the identification of some geese breeds.

Key words: goose, DNA bar code, sequence analysis, tRNA

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