钱塘江三角鲂线粒体基因组测序及其结构特征分析

doi:10.3969/j.issn.1004-1524.2020.09.08

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1591-1608.DOI: 10.3969/j.issn.1004-1524.2020.09.08

钱塘江三角鲂线粒体基因组测序及其结构特征分析

刘凯, 冯晓宇, 马恒甲, 谢楠^*

杭州市农业科学研究院水产研究所,浙江杭州 310024

收稿日期:2020-03-02 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *谢楠,E-mail:n.xie@163.com
作者简介:刘凯(1982—),男,安徽马鞍山人,硕士,高级工程师,主要从事鱼类遗传与免疫研究。E-mail:liukai0106@hz.cn
基金资助:
国家大宗淡水鱼产业技术体系杭州综合试验站项目(CARS-45-39);杭州市农业科研主动设计项目(20162012A03);杭州市农科院创新基金(2019HNCT-01)

Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River

LIU Kai, FENG Xiaoyu, MA Hengjia, XIE Nan^*

Institute of Fishery Science, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2020-03-02 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为了研究钱塘江流域三角鲂(Megalobrama terminalis Richardson)线粒体基因组结构特征及鲌亚科鱼类的系统进化关系,通过PCR扩增、测序、软件拼接获得了钱塘江三角鲂线粒体基因组全序列,GenBank登录号为MN725725。结果表明,钱塘江三角鲂线粒体基因组序列全长为16 621 bp,碱基组成分别为A(31.23%)、G(16.17%)、C(27.87%)和T(24.73%);共有13个蛋白编码基因,22个tRNA基因,2个rRNA基因,NAD6、tRNA-Gln、tRNA-Ala、tRNA-Asn、tRNA-Cys、tRNA-Tyr、tRNA-Ser^(UCN)、tRNA-Glu和tRNA-Pro等基因编码在L链上,其余基因均在H链上编码。钱塘江三角鲂线粒体基因组全序列与蛋白编码基因的A+T含量分别为55.97%和55.86%,具有明显的AT偏好性。线粒体基因中存在2个散在重复序列,分别位于线粒体控制区中终止结合序列区的前端和控制区3'的末端。在22个tRNA基因中,除了tRNA-Ser^(AGY)外,均具有典型的三叶草二级结构。基于BLAST比较,钱塘江三角鲂与黑龙江流域的三角鲂一致性为99.76%,与珠江流域三角鲂一致性为99.87%。基于15种隶属于7属的鲌亚科鱼类线粒体基因组全序列构建的系统进化树,鲂属与鳊属亲缘关系比与鲌属的亲缘关系上较近,与属、半属和细鳊属亲缘关系上较远。

关键词: 三角鲂, 线粒体基因组, 序列分析, 系统进化树

Abstract: To study the mitochondrial genomic structure of Megalobrama terminalis Richardson from Qiantang River and phylogenetic relationship of Cultrinae subfamily, the amplification by PCR, sequencing, and software splicing were used to obtain complete mitochondrial genome of M. terminalis from Qiantang River, whose GenBank accession number was MN725725. The full-length sequence of the mitochondrial genome of M. terminalis from Qiantang River was 16 621 bp, and its base composition was A (31.23%), G (16.17%), C (27.87%), and T (24.73%). There were 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes in the mitochondrial genome. Except for NAD6, tRNA-Gln, tRNA-Ala, tRNA-Asn, tRNA-Cys, tRNA-Tyr, tRNA-Ser^(UCN), tRNA-Glu, and tRNA-Pro, the other genes were encoded on the H chain. The A+T content of the mitochondrial genome and protein-coding genes in the mitochondria of M. terminalis from Qiangjiang River was 55.97% and 55.86%, respectively. The mitochondrial genome, and protein-coding genes in the mitochondria had obvious AT preference. There were two interspersed repeats in the mitochondrial gene, which were located at the front end of the terminal associated sequences region and the 3'end of the control region in the mitochondrial control region, respectively. Of the 22 tRNA genes, except tRNA-Ser^(AGY), they had a typical clover secondary structure. Based on the comparison of BLAST, the consistency of M. terminalis from Qiantang River and Heilongjiang River was 99.76%, while the consistency of M. terminalis from Pearl River was 99.87%. Phylogenetic tree was constructed based on complete mitochondrial genomes of 15 species of Cultrinae subfamily belonging to 7 genera. The genetic relationship between Megalobrama and Parabramis was closer than that of Culter, and Megalobrama was far away from Hemiculter, Hemiculterella, and Metzia.

Key words: Megalobrama terminalis, mitochondrial genome, sequence analysis, phylogenetic tree

中图分类号:

刘凯, 冯晓宇, 马恒甲, 谢楠. 钱塘江三角鲂线粒体基因组测序及其结构特征分析[J]. 浙江农业学报, 2020, 32(9): 1591-1608.

LIU Kai, FENG Xiaoyu, MA Hengjia, XIE Nan. Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River[J]. , 2020, 32(9): 1591-1608.

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钱塘江三角鲂线粒体基因组测序及其结构特征分析

Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River

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