鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响

doi:10.3969/j.issn.1004-1524.2020.09.09

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1609-1614.DOI: 10.3969/j.issn.1004-1524.2020.09.09

鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响

桂雪儿¹, 王志¹, 李思婷¹, 贺濛初¹, 朱杰², 冯士彬¹, 吴金节^1,*

1.安徽农业大学动物科技学院,安徽合肥 230061;
2.宣城木子禽业专业合作社,安徽宣城 242000

收稿日期:2019-12-30 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *吴金节,E-mail:wjj@ahau.edu.cn
作者简介:桂雪儿(1997—),女,安徽合肥人,硕士研究生,研究方向为畜禽健康养殖。E-mail:2323876532@qq.com
基金资助:
安徽省重点研究与开发计划(1804a07020135)

Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers

GUI Xueer¹, WANG Zhi¹, LI Siting¹, HE Mengchu¹, ZHU Jie², FENG Shibin¹, WU Jinjie^1,*

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China;
2. Xuancheng Muzi Poultry Cooperative, Xuancheng 242000, China

Received:2019-12-30 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为研究鸡源复合益生菌对青年白羽肉杂鸡血清免疫球蛋白和肠道Toll样受体通路的影响,将180只健康的28日龄白羽肉杂鸡随机分为A(基础饲粮)、B(基础饲粮中添加10¹¹ CFU·kg^-1益生菌)、C(基础饲粮中添加2×10¹¹ CFU·kg^-1益生菌)3个处理,每个处理3个重复,每个重复20只鸡。试验期21 d。结果表明:与A组相比,B组和C组鸡血清中IgG、IgM水平显著(P<0.05)升高,B组和C组鸡肠道TLR2、TLR4、Myd88、TRAF-6、AP-1蛋白表达及mRNA相对表达量显著(P<0.05)升高。与B组相比,C组鸡血清IgG、IgM、IgA水平均无明显差异(P>0.05),鸡肠道TLR4、Myd88蛋白表达显著(P<0.05)升高,Myd88基因mRNA相对表达量显著(P<0.05)升高。综上,添加不同浓度的鸡源复合益生菌能够提高白羽肉杂鸡血清中IgG、IgM水平,通过Toll样受体通路增强肉鸡的免疫功能。

关键词: 乳酸菌, 枯草芽孢杆菌, 白羽肉杂鸡, 免疫球蛋白, Toll样受体通路

Abstract: To investigate the effects of probiotics on serum immunoglobulin and intestinal Toll-like receptor pathway in White feather broiler, one hundred and eighty 28-day-old white feather hybrid broilers were randomly divided into group I (basic diet), group II (basic diet adding 10¹¹ CFU·kg^-1 probiotics) and group III ((basic die adding 2×10¹¹ CFU·kg^-1 probiotics), with 3 repeats in each group and 20 broilers in each repeat. The experimental period was 21 d. The results showed that, compared with group A, the serum IgG and IgM levels in group B and group C increased significantly (P<0.05). The protein expression of TLR2, TLR4, Myd88, TRAF-6, AP-1 and their relative expression of mRNA in group B and group C were significantly (P<0.05) increased. Compared with group B, the serum IgG, IgM and IgA levels in group C were no significant difference (P>0.05). The protein expression of TLR4 and MyD88 in group C were significantly (P<0.05) increased, and the relative expression of MyD88 mRNA was significantly (P<0.05) increased. Feeding probiotics can improve the serum IgG and IgM levels of white feather broilers, and regulate the immune function of broiler intestine effectively through toll like receptor pathway.

Key words: lactic acid bacteria, Bacillus subtilis, white feather broiler, immunoglobulin, toll-like receptor pathway

中图分类号:

S831

桂雪儿, 王志, 李思婷, 贺濛初, 朱杰, 冯士彬, 吴金节. 鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响[J]. 浙江农业学报, 2020, 32(9): 1609-1614.

GUI Xueer, WANG Zhi, LI Siting, HE Mengchu, ZHU Jie, FENG Shibin, WU Jinjie. Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers[J]. , 2020, 32(9): 1609-1614.

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鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响

Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers

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