戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化

doi:10.3969/j.issn.1004-1524.2020.02.17

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (2): 327-336.DOI: 10.3969/j.issn.1004-1524.2020.02.17

戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化

王明哲^1,2, 杨颖², 唐伟敏², 刘哲², 孙培龙¹, 陆胜民^2,*

1.浙江工业大学海洋学院,浙江杭州 310014;
2.浙江省农业科学院食品科学研究所,浙江杭州 310021

收稿日期:2019-08-15 出版日期:2020-02-25 发布日期:2020-03-13
通讯作者: *陆胜民,E-mail:lushengmin@hotmail.com
作者简介:王明哲(1995—),男,江苏高邮人,硕士研究生,研究方向为食品微生物。E-mail: 631450238@qq.com
基金资助:
浙江省重点研发计划(2018C02045)

Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112

WANG Mingzhe^1,2, YANG Ying², TANG Weimin², LIU Zhe², SUN Peilong¹, LU Shengmin^2,*

1.Ocean College, Zhejiang University of Technology, Hangzhou 310014,China;
2. Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-08-15 Online:2020-02-25 Published:2020-03-13

摘要/Abstract

摘要： 为提高戊糖乳杆菌YY112 胞外多糖的产量,本研究结合单因素试验、正交试验、Box-Behnken响应面试验设计,对该菌株产多糖的营养条件和发酵工艺进行优化。结果表明:在本次研究的范围内,MRS培养基为适宜的基础培养基;蔗糖为适宜碳源,添加量为质量体积比3%;大豆蛋白胨为适宜氮源,添加量为质量体积比1%;酵母粉能够提高菌株的多糖产量,适宜添加量为质量体积比2%;培养基初始pH值为6.0,菌株接种量为体积比4%,适宜培养温度29.5 ℃,此时培养24.5 h胞外多糖产量最高。在此优化培养条件下,YY112胞外多糖产量达到(380.97±0.45)mg·L^-1,与响应面分析预测值基本吻合,较优化前增加了46.52%。

关键词: 乳酸菌, 胞外多糖, 营养条件, 工艺条件, 优化

Abstract: In order to improve the yield of exopolysaccharide produced by Lactobacillus pentosae YY112, the nutritional conditions and fermentation technology of the strain were optimized by single factor test, orthogonal test and Box-Behnken response surface design. The results showed that: within the scope of this study, MRS medium was the appropriate basic one, using 3% (m/V) sucrose was the appropriate carbon source, 1% (m/V) soybean peptone was the appropriate nitrogen source and 2% (m/V) yeast powder could increase polysaccharide yield of the strain. The initial pH value of the medium was 6.0, and the inoculation amount of the strain was 4% (V/V). The optimum fermentation temperature was 29.5 ℃, and the best fermentation time was 24.5 h. Under the optimized culture conditions, the yield of exopolysaccharide reached (380.97±0.45) mg·L^-1, which was basically consistent with the predicted value of response surface analysis, an increase of 46.52% compared with that before optimization.

Key words: lactic acid bacteria, exopolysaccharide, nutritional condition, technological condition, optimization

中图分类号:

TS201.3

王明哲, 杨颖, 唐伟敏, 刘哲, 孙培龙, 陆胜民. 戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化[J]. 浙江农业学报, 2020, 32(2): 327-336.

WANG Mingzhe, YANG Ying, TANG Weimin, LIU Zhe, SUN Peilong, LU Shengmin. Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112[J]. , 2020, 32(2): 327-336.

参考文献

[1] 牛萌萌, 王坤, 鹿保鑫. 乳酸菌胞外多糖的研究进展[J]. 黑龙江八一农垦大学学报, 2018, 30(3): 40-45.
NIU M M, WANG K, LU B X.Research progress of lactic acid bacteria extracellular polysaccharide[J]. Journal of Heilongjiang Bayi Agricultural University, 2018, 30(3): 40-45.(in Chinese with English abstract)
[2] RUAS-MADIEDO P, HUGENHOLTZ J, ZOON P.An overview of the functionality of exopolysaccharides produced by lactic acid Bacteria[J]. International Dairy Journal, 2002, 12(2/3): 163-171.
[3] 张晓黎, 吴兴壮, 张华. 乳酸菌功能作用及其在生产中的应用[J]. 农业工程技术, 2015, 35(14): 36-39.
ZHANG X L, WU X Z, ZHANG H.Function and application of lactic acid bacteria in production[J]. Agriculture Engineering Technology(Agricultural Product Processing Industry), 2015, 35(14): 36-39.(in Chinese)
[4] 杜瑞平, 郭帅, 潘娜, 等. 乳酸菌胞外多糖益生功能研究进展[J]. 动物营养学报, 2018, 30(7): 2439-2444.
DU R P, GUO S, PAN N, et al.Research progress on probiotic function of lactic acid bacteria exopolysaccharides[J]. Chinese Journal of Animal Nutrition, 2018, 30(7): 2439-2444.(in Chinese with English abstract)
[5] 邸维, 张兰威, 易华西, 等. 乳酸菌胞外多糖结构及其功能多样性的研究进展[J]. 中国乳品工业, 2017, 45(5): 32-37.
DI W, ZHANG L W, YI H X, et al.Research advances on structure and diversity function of exopolysaccharides produced by lactic acid bacteria[J]. China Dairy Industry, 2017, 45(5): 32-37.(in Chinese with English abstract)
[6] 朱奇, 郭善利, 陈彦, 等. 酸乳中胞外多糖(EPS)产生条件的研究[J]. 食品科技, 2004, 29(4): 18-19, 28.
ZHU Q, GUO S L, CHEN Y, et al.Study on conditions of producing exopolysaccharides from yoghurt[J]. Food Science and Technology, 2004, 29(4): 18-19, 28.(in Chinese with English abstract)
[7] VUYST D, VANDERVEKEN, VAN DE VEN, et al. Production by and isolation of exopolysaccharides from Streptococcus thermophilus grown in a milk medium and evidence for their growth-associated biosynthesis[J]. Journal of Applied Microbiology, 1998, 84(6): 1059-1068.
[8] 于静, 曾小群, 王鸿飞, 等. 高产胞外多糖乳酸菌的筛选及其发酵条件优化[J]. 中国食品学报, 2015, 15(2): 93-98.
YU J, ZENG X Q, WANG H F, et al.Screening of lactic acid bacteria with higher yield of exopolysaccharide and optimization of fermentation conditions[J]. Journal of Chinese Institute of Food Science and Technology, 2015, 15(2): 93-98.(in Chinese with English abstract)
[9] 马静, 裴家伟, 吴荣荣, 等. 影响乳酸菌胞外多糖产量的因素研究[J]. 中国乳品工业, 2003, 31(5): 12-16.
MA J, PEI J W, WU R R, et al.Studies on factors contributing to the production of exopolysaccharides by lactic acid bacteria[J]. China Dairy Industry, 2003, 31(5): 12-16.(in Chinese with English abstract)
[10] 董群, 郑丽伊, 方积年. 改良的苯酚-硫酸法测定多糖和寡糖含量的研究[J]. 中国药学杂志, 1996, 31(9): 550-553.
DONG Q, ZHENG L Y, FANG J N.Modifiedphenol-sulfuric acid method for determination of the content of oligo- and poly-saccharides[J]. Chinese Pharmaceutical Journal, 1996, 31(9): 550-553.(in Chinese with English abstract)
[11] 李志成, 严佩峰, 李红蕊, 等. 乳酸菌基础培养基比较研究[J]. 食品研究与开发, 2007, 28(11): 71-74.
LI Z C, YAN P F, LI H R, et al.Study on comparison of basic culture medium of lactic acid bacteria[J]. Food Research and Development, 2007, 28(11): 71-74.(in Chinese with English abstract)
[12] MOZZI F, ROLLáN G, GIORI G S D, et al. Effect of galactose and glucose on the exopolysaccharide production and the activities of biosynthetic enzymes in Lactobacillus casei CRL 87[J]. Journal of Applied Microbiology, 2010, 91(1):160-167.
[13] 李莉, 张赛, 何强, 等. 响应面法在试验设计与优化中的应用[J]. 实验室研究与探索, 2015, 34(8): 41-45.
LI L, ZHANG S, HE Q, et al.Application of response surface methodology in experiment design and optimization[J]. Research and Exploration in Laboratory, 2015, 34(8): 41-45.(in Chinese with English abstract)
[14] 顾瑞霞. 乳酸菌胞外多糖生物合成及生理功能特性的研究[D]. 哈尔滨: 东北农业大学, 2000.
GU R X.Studies on synthesis and physiological functions of exopolysaccharides of lactic acid bacteria[D]. Harbin: Northeast Agricultural University, 2000.(in Chinese with English abstract)
[15] GARCIA-GARIBAY M, MARSHALL V M E. Polymer production by Lactobacillus delbrueckii ssp. bulgaricus[J]. Journal of Applied Bacteriology, 1991, 70(4): 325-328.
[16] CERNING J, BOUILLANNE C, DESMAZEAUD M J, et al.Exocellular polysaccharide production by Streptococcus thermophilus[J]. Biotechnology Letters, 1988, 10(4): 255-260.
[17] KIMMEL S A.Optimisation of exopolysaccharide production by Lactobacillus delbrueckii subsp. Bulgaricus RR grown in a semidefined medium[J]. Applied & Environmental Microbiology, 1998, 64(2):659-664.
[18] 孙长胜, 伍业旭, 李啸. 酵母浸粉在酸奶发酵中的应用研究[J]. 现代食品, 2015(24): 59-61.
SUN C S, WU Y X, LI X.Application of yeast extract in yogurt fermentation[J]. Modern Food, 2015(24): 59-61.(in Chinese with English abstract)
[19] BERG D V D, ROBIJN G W, JANSSEN A C, et al. Production of a novel extracellular polysaccharide by Lactobacillus sake 0-1 and characterization of the polysaccharide.[J]. Applied & Environmental Microbiology, 1995, 61(8):2840-2844.
[20] GASSEM M A, SCHMIDT K A, FRANK J F.Exopolysaccharide production from whey lactose by fermentation with Lactobacillus delbrueckii ssp. bulgaricus[J]. Journal of Food Science, 2010, 62(1):171-173.
[21] CERNING J.Exocellular polysaccharides produced by lactic acid bacteria[J]. FEMS Microbiology Letters, 1990, 87(1/2): 113-130.
[22] 冯美琴, 邢家溧, 张琦, 等. 植物乳杆菌胞外多糖发酵条件的优化[J]. 食品科学, 2011, 32(23): 215-219.
FENG M Q, XING J L, ZHANG Q, et al.Optimization of fermentation conditions for production of exopolysaccharides by Lactobacillus plantarum 70810[J]. Food Science, 2011, 32(23): 215-219.(in Chinese with English abstract)
[23] FERREIRA S L C, BRUNS R E, FERREIRA H S, et al. Box-Behnken design: an alternative for the optimization of analytical methods[J]. Analytica Chimica Acta, 2007, 597(2): 179-186.

戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化

Optimization of fermentation techniques on yield of exopolysaccharide produced by Lactobacillus pentosae YY112

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	岳登高, 程亮, 郭青云. 出芽短梗霉菌PA-2脂肽类物质抑菌活性及其发酵条件优化[J]. 浙江农业学报, 2021, 33(3): 479-489.
[2]	忻晓庭, 刘大群, 张程程, 吴敏, 陈登高, 章检明. 我国特色发酵蔬菜降解亚硝酸盐菌株的筛选鉴定及应用[J]. 浙江农业学报, 2021, 33(2): 335-345.
[3]	桂雪儿, 王志, 李思婷, 贺濛初, 朱杰, 冯士彬, 吴金节. 鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响[J]. 浙江农业学报, 2020, 32(9): 1609-1614.
[4]	胡坚, 胡峰俊, 张红, 朱颖. 基于改进布谷鸟搜索算法对水质监测无线传感器部署的优化[J]. 浙江农业学报, 2020, 32(5): 897-903.
[5]	温慧萍, 雷伟敏, 柯乐芹, 陈雨薇, 侯景, 肖建中. 灵芝孢子粉总三萜的提取优化及其体外生物活性[J]. 浙江农业学报, 2020, 32(4): 705-713.
[6]	张红涛, 李艺嘉, 谭联, 许帅涛. 基于CS-SVM的谷子叶片病害图像识别[J]. 浙江农业学报, 2020, 32(2): 274-282.
[7]	陈燕丽, 唐志鹏, 欧克纬, 卢业飞, 张宇. 金柑CDDP-PCR反应体系优化研究[J]. 浙江农业学报, 2020, 32(1): 65-71.
[8]	宋朱军, 吴雪梅, 李国昌, 王芳, 刘红芸, 张富贵, 张培坤. 底肥定点深施机地轮组件优化[J]. 浙江农业学报, 2019, 31(5): 829-836.
[9]	范铭, 向露, 刘哲, 陆胜民. 基于体外降糖活性的桑葚渣水提物的超声辅助工艺优化[J]. 浙江农业学报, 2019, 31(3): 480-486.
[10]	李颀, 赵洁, 杨柳, 王俊, 高一星. 基于GA-BP神经网络和特征向量优化组合的黄瓜叶片病斑识别[J]. 浙江农业学报, 2019, 31(3): 487-495.
[11]	马露, 孔维洲, 王丽萍, 张喜康, 王聪, 李佩佩, 刘敦华. 响应面法优化发酵鹿肉干工艺及其货架期预测[J]. 浙江农业学报, 2019, 31(12): 2109-2119.
[12]	张文平, 王清, 黄诗宸, 吴佩佳, 程新. 乳酸菌胞外多糖对水稻生长及土壤理化性质的影响[J]. 浙江农业学报, 2019, 31(1): 130-138.
[13]	陈志, 汪一婷, 吕永平, 牟豪杰, 陈剑平. 铁皮石斛无菌播种成苗体系优化[J]. 浙江农业学报, 2018, 30(11): 1870-1878.
[14]	王储炎, 程俊文, 祝超. 黑芝菌发酵产生胞外多糖的工艺优化及其抗氧化活性[J]. 浙江农业学报, 2018, 30(11): 1938-1945.
[15]	储婷婷, 朱德泉, 朱宏, 张顺, 蒋锐, 张俊. 螺旋轴式移箱机构多学科协同优化与试验[J]. 浙江农业学报, 2018, 30(11): 1946-1957.