乳酸菌胞外多糖对水稻生长及土壤理化性质的影响

doi:10.3969/j.issn.1004-1524.2019.01.17

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 130-138.DOI: 10.3969/j.issn.1004-1524.2019.01.17

乳酸菌胞外多糖对水稻生长及土壤理化性质的影响

张文平, 王清, 黄诗宸, 吴佩佳, 程新^*

江西农业大学生物科学与工程学院,江西南昌 330045

收稿日期:2018-03-23 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 程新,E-mail: xincheng@jxau.edu.cn
作者简介:张文平(1993—),男,江西新余人,硕士研究生,主要从事农业资源微生物方向的研究。E-mail: 984360913@qq.com
基金资助:
国家自然科学基金(31460469); 江西农业大学大学生创新创业训练计划(201710410070)

Effects of exopolysaccharides of lactic acid bacteria on rice growth and soil physicochemical properties

ZHANG Wenping, WANG Qing, HUANG Shichen, WU Peijia, CHENG Xin^*

College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China

Received:2018-03-23 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 为探讨微生物多糖在促进作物生长上的应用效果及其作用机理,以水稻准两优608为供试材料,将不同浓度(100、1 000 mg·L^-1)乳酸菌胞外多糖施加于土壤,同时以相同浓度的葡萄糖作为对照,研究乳酸菌胞外多糖对水稻秧苗生物学性状及水稻根际土壤理化性质的影响,同时考查微生物多糖与单糖在水稻促生效果上的差异。结果表明:在土壤中添加不同浓度的乳酸菌多糖能促进水稻幼苗的生长,显著改善土壤结构,提高土壤团聚体稳定性,缓解土壤酸化,提高土壤酶活性,同时对土壤养分也有明显的改善效果。相关性分析表明,外源物质介导的土壤环境因子变化与水稻的生长作用关联极为密切。主成分分析结果证明,乳酸菌胞外多糖对水稻生长及土壤环境因子的作用效果与葡萄糖存在明显区别。乳酸菌胞外多糖可以作为一种新型植物生长调节剂应用于农业生产。

关键词: 乳酸菌, 胞外多糖, 水稻, 土壤

Abstract: To explore the application potential of microbial polysaccharides and its regulatory mechanism on crop growth, rice cultivar Zhunliangyou 608 was used to investigate the effects of exopolysaccharides (EPS) from lactic acid bacteria on biological traits of rice seedlings and physicochemical properties of rice rhizosphere soil. It was shown that application of EPS could promote the growth of rice seedlings and improve rhizosphere soil environment, enhance soil structure, improve soil aggregate stability, mitigate soil acidification, increase soil enzyme activities, and improve soil nutrients. Correlation analysis and principal component analysis showed that the mechanism of rice growth promotion by exopolysaccharides was different from that by glucose. The exopolysaccharides of lactic acid bacteria could be used as a new plant growth regulator.

Key words: lactic acid bacteria, exopolysaccharides, rice, soil

中图分类号:

S153
S482.8

张文平, 王清, 黄诗宸, 吴佩佳, 程新. 乳酸菌胞外多糖对水稻生长及土壤理化性质的影响[J]. 浙江农业学报, 2019, 31(1): 130-138.

ZHANG Wenping, WANG Qing, HUANG Shichen, WU Peijia, CHENG Xin. Effects of exopolysaccharides of lactic acid bacteria on rice growth and soil physicochemical properties[J]. , 2019, 31(1): 130-138.

参考文献

[1] COMPANT S, CLÉMENT C, SESSITSCH A. Plant growth-promoting bacteria in the rhizo-and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization[J]. Soil Biology and Biochemistry, 2010, 42(5): 669-678.
[2] HARDOIM P R, HVAN OVERBEEK L S, HVAN ELSAS J D. Properties of bacterial endophytes and their proposed role in plant growth[J]. Trends in Microbiology, 2008, 16(10): 463-471.
[3] SANDHYA V, ALI S Z.The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation[J]. Microbiology, 2015, 84(4): 512-519.
[4] 闫亚南. 产胞外多糖植物促生菌对小麦高效利用钾肥的影响及机制研究[D].南京:南京农业大学, 2012.
YAN Y N.Effect of polysaccharide-producing plant growth-promoting bacteria on potassium unitization of wheat and the mechanism[D]. Nanjing: Nanjing Agricultural University, 2012. (in Chinese with English abstract)
[5] XU Y, ROSSI F, COLICA G, et al.Use of cyanobacterial polysaccharides to promote shrub performances in desert soils: a potential approach for the restoration of desertified areas[J]. Biology and Fertility of Soils, 2013, 49(2): 143-152.
[6] 马晓颖, 杨镇, 宋艳雨,等. 不同微生物多糖对植物生长和植保素累积的影响[J]. 辽宁农业科学, 2016 (2): 23-26.
MA X Y, YANG Z, SONG Y Y, et al.The effects of different kinds of microbial polysaccharides on plant growth and phytoalexin accumulation[J]. Liaoning Agricultural Sciences, 2016 (2): 23-26. (in Chinese with English abstract)
[7] YANG Z, LI S, ZHANG X, et al.Capsular and slime-polysaccharide production by Lactobacillus rhamnosus JAAS8 isolated from Chinese sauerkraut: potential application in fermented milk products[J]. Journal of Bioscience and Bioengineering, 2010, 110(1): 53-57.
[8] RIMADA P S, ABRAHAM A G.Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey[J]. Le Lait, 2003, 83(1): 79-87.
[9] 周虎, 吕贻忠, 杨志臣, 等. 保护性耕作对华北平原土壤团聚体特征的影响[J]. 中国农业科学, 2007, 40(9): 1973-1979.
ZHOU H, LÜ Y Z, YANG Z C, et al.Effects of conservation tillage on soil aggregates in Huabei Plain, China[J]. Scientia Agricultura Sinica, 2007, 40(9): 1973-1979. (in Chinese with English abstract)
[10] 上官王丽. 产胞外多糖细菌多样性及其对土壤团聚体形成作用的研究[D]. 南京: 南京农业大学, 2013.
SHANGGUAN W L.The diversity of exopolysaccharide-producing bacteria and their effects on aggregation in soils[D]. Nanjing: Nanjing Agricultural University, 2013. (in Chinese with English abstract)
[11] 鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000: 25-114.
[12] 关松荫. 土壤酶及其研究法[M]. 北京: 农业出版社, 1986: 274-340.
[13] 杨兰芳, 曾巧, 李海波, 等. 紫外分光光度法测定土壤过氧化氢酶活性[J]. 土壤通报, 2011, 42(1): 207-210.
YANG L F, ZENG Q, LI H B, et al.Measurement of catalase activity in soil by ultraviolet spectrophotometry[J]. Chinese Journal of Soil Science, 2011, 42(1): 207-210. (in Chinese with English abstract)
[14] 吕虹瑞, 吕俊平, 刘琪, 等. 太原汾河蓄水区2014年丰水期叶绿素的时空变化及环境因子相关性分析[J]. 水生生物学报, 2016, 40(6): 1278-1286.
LÜ H R, LÜ J P, LIU Q, et al.Temporal and spatial variation of chlorophyll and the correlation analysis with environmental factors in the Taiyuan Fenhe Water Reserve area in flooding season in 2014[J]. Acta Hydrobiologica Sinica, 2016, 40(6): 1278-1286. (in Chinese with English abstract)
[15] ROLLAND F, MOORE B, SHEEN J.Sugar sensing and signaling in plants[J]. The Plant Cell, 2002, 14(Suppl.): S185-S205.
[16] PRAXEDES S C, DAMATTA F M, LOUREIRO M E, et al.Effects of long-term soil drought on photosynthesis and carbohydrate metabolism in mature robusta coffee (Coffea canephora Pierre var. kouillou) leaves[J]. Environmental & Experimental Botany, 2006, 56(3): 263-273.
[17] 赵莹, 杨克军, 赵长江, 等. 外源糖调控玉米光合系统和活性氧代谢缓解盐胁迫[J]. 中国农业科学, 2014, 47(20): 3962-3972.
ZHAO Y, YANG K J, ZHAO C J, et al.Alleviation of the adverse effects of salt stress by regulating photosynthetic system and active oxygen metabolism in maize seedlings[J]. Scientia Agricultura Sinica, 2014, 47(20): 3962-3972. (in Chinese with English abstract)
[18] 王玉萍, 于丹, 李成, 等. 壳聚糖对盐胁迫下小麦种子萌发及幼苗生理特性的影响[J]. 干旱地区农业研究, 2016, 34(1): 180-185.
WANG Y P, YU D, LI C, et al.Effect of chitosan on seed germination and seedling physiological characters of wheat under salt stress[J]. Agricultural Research in the Arid Areas, 2016, 34(1): 180-185. (in Chinese with English abstract)
[19] 李建伟, 于立河, 郭伟, 等. 外源糖氮调控对春小麦花后旗叶光合特性和物质转运的影响[J]. 作物杂志, 2015 (2): 107-110.
LI J W, YU L H, GUO W, et al.Effects of exogenous sugar and nitrogen on photosynthetic characteristics and assimilate translocation in flag leaf post-anthesis of spring wheat[J]. Crops, 2015 (2): 107-110. (in Chinese with English abstract)
[20] 胡梦芸, 李辉, 张颖君, 等. 水分胁迫下葡萄糖对小麦幼苗光合作用和相关生理特性的影响[J]. 作物学报, 2009, 35(4): 724-732.
HU M Y, LI H, ZHANG Y J, et al.Photosynthesis and related physiological characteristics affected by exogenous glucose in wheat seedlings under water stress[J]. Acta Agronomica Sinica, 2009, 35(4): 724-732. (in Chinese with English abstract)
[21] 周文杰, 张鹏, 秦嗣军, 等. 添加葡萄糖和淀粉对盆栽甜樱桃根区土壤碳代谢及根功能的影响[J]. 应用生态学报, 2015, 26(11): 3300-3308.
ZHOU W J, ZHANG P, QIN S J, et al.Effects of exogenous glucose and starch on soil carbon metabolism of root zone and root function in potted sweet cherry[J]. Chinese Journal of Applied Ecology, 2015, 26(11): 3300-3308. (in Chinese with English abstract)
[22] 艾雪. 沙漠结皮中耐盐碱细菌的分离及其固沙特性研究[D]. 兰州: 兰州交通大学, 2015.
AI X.Isolation of saline-alkali-tolerant bacterial strains from desert crust and its characteristics in sand fixation[D]. Lanzhou: Lanzhou Jiaotong University, 2015. (in Chinese with English abstract)
[23] 曾勇军, 周庆红, 吕伟生, 等. 土壤酸化对双季早、晚稻产量的影响[J]. 作物学报, 2014, 40(5): 899-907.
ZENG Y J, ZHOU Q H, LÜ W S, et al.Effects of soil acidification on the yield of double season rice[J]. Acta Agronomica Sinica, 2014, 40(5): 899-907. (in Chinese with English abstract)
[24] 陈平平, 郭莉莉, 唐利忠, 等. 土壤pH对不同酸性敏感型水稻品种氮利用效率与根际土壤生物学特性的影响[J]. 核农学报, 2017, 31(4): 757-767.
CHEN P P, GUO L L, TANG L Z, et al.Effect of soil pH on nitrogen utilization efficiency and biological characters of rhizosphere soil of rice varieties with different acid-sensitivity[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(4): 757-767. (in Chinese with English abstract)
[25] 崔文明. 乙酰化葡萄糖对盐碱土理化性质及作物生长影响机制的研究[D]. 太谷:山西农业大学, 2015.
CUI W M.The influence of acetyl glucose on saline alkali soil physical and chemical properties and crop growth[D]. Taigu: Shanxi Agricultural University, 2015. (in Chinese with English abstract)
[26] 刘学东, 陈林, 杨新国,等. 放牧与围栏对荒漠草原土壤养分及酶活性的影响[J]. 浙江农业学报, 2016, 28(8):1389-1395.
LIU X D, CHEN L, YANG X G, et al.Effects of grazing and fencing on nutrients and enzyme activities in desert steppe soil[J]. Acta Agriculturae Zhejiangensis, 2016, 28(8):1389-1395. (in Chinese with English abstract)
[27] 陈心想, 耿增超, 王森, 等. 施用生物炭后塿土土壤微生物及酶活性变化特征[J]. 农业环境科学学报, 2014, 33(4):751-758.
CHEN X X, GENG Z C, WANG S, et al.Effects of biochar amendment on microbial biomass and enzyme activities in loess soil[J]. Journal of Agro-Environment Science, 2014, 33(4):751-758. (in Chinese with English abstract)
[28] 孙姗姗. 水分胁迫下糖类浸种对玉米萌发及根际微生物的影响[D]. 沈阳:沈阳农业大学, 2016.
SUN S S.Under water stress carbohydrate soaking on germination of maize and rhizosphere microorganisms[D]. Shen-yang: Shenyang Agricultural University, 2016. (in Chinese with English abstract)

乳酸菌胞外多糖对水稻生长及土壤理化性质的影响

Effects of exopolysaccharides of lactic acid bacteria on rice growth and soil physicochemical properties

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	范琳娟, 刘子荣, 徐雪亮, 王奋山, 彭德良, 姚英娟. 6种杀线剂对重茬山药土壤微生物数量、酶活性和养分含量的影响[J]. 浙江农业学报, 2021, 33(3): 506-515.
[2]	陈丹, 汤翠凤, 董超, 甘树仙, 李俊, 阿新祥, 张斐斐, 杨雅云, 牛赛赛, 戴陆园. 云南软米地方品种籽粒淀粉品质特性研究[J]. 浙江农业学报, 2021, 33(2): 203-214.
[3]	程晶, 刘济明, 王姝, 王灯, 李丽霞, 徐国瑞, 陈梦, 黄路婷. 喀斯特特有植物罗甸小米核桃响应土壤水分的表型可塑性[J]. 浙江农业学报, 2021, 33(2): 259-269.
[4]	范琳娟, 刘子荣, 徐雪亮, 王奋山, 彭德良, 姚英娟. 山药不同种植模式对土壤线虫群落结构和土壤理化性质的影响[J]. 浙江农业学报, 2021, 33(2): 316-325.
[5]	忻晓庭, 刘大群, 张程程, 吴敏, 陈登高, 章检明. 我国特色发酵蔬菜降解亚硝酸盐菌株的筛选鉴定及应用[J]. 浙江农业学报, 2021, 33(2): 335-345.
[6]	邹文雄, 吴伟, 关亚静, 曹栋栋, 卞晓波, 施德云, 丁丽玲. 水稻种子休眠调控技术研究进展[J]. 浙江农业学报, 2021, 33(2): 369-379.
[7]	杨乙未, 肖华, 陈浒, 肖聶佳, 郭城. 喀斯特地区不同玫瑰混农林模式的土壤螨类群落结构特征[J]. 浙江农业学报, 2021, 33(1): 112-121.
[8]	陈贵, 鲁晨妮, 石艳平, 倪雄伟, 程旺大, 张红梅, 王保君, 张丽萍, 孙达. 不同缓控释肥搭配脲铵对水稻产量、氮素利用效率和土壤养分的影响[J]. 浙江农业学报, 2021, 33(1): 122-130.
[9]	桂雪儿, 王志, 李思婷, 贺濛初, 朱杰, 冯士彬, 吴金节. 鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响[J]. 浙江农业学报, 2020, 32(9): 1609-1614.
[10]	李金武, 郁继华, 吕剑, 冯致, 杨海兴, 车旭升, 秦启杰, 张洋, 金宁. 不同覆盖方式对高原夏季露地松花菜产量、品质和土壤养分的影响[J]. 浙江农业学报, 2020, 32(9): 1626-1633.
[11]	常会庆, 徐富锦, 潘亚杰. 碳酸钙及其与壳聚糖联用对石灰性土壤铬污染的钝化效应[J]. 浙江农业学报, 2020, 32(9): 1665-1671.
[12]	张统帅, 闫丽娟, 李广, 陈国鹏, 罗永忠. 免耕和秸秆覆盖对旱作区土壤氮素、水分和春小麦产量的影响[J]. 浙江农业学报, 2020, 32(8): 1329-1341.
[13]	卢牧原, 刘源, 刘桂建. 基于组合模型的黑土区土壤有机质含量预测分析[J]. 浙江农业学报, 2020, 32(8): 1427-1436.
[14]	钱家炜, 刘晓青, 张静静, 周卫红, 李建龙. 张家港市农田土壤重金属含量高光谱遥感监测模型构建[J]. 浙江农业学报, 2020, 32(8): 1437-1445.
[15]	谷建诚, 郭彬, 林义成, 傅庆林, 刘琛, 丁能飞, 李华, 李凝玉. 根表铁膜对水稻镉吸收的影响[J]. 浙江农业学报, 2020, 32(6): 963-970.