荞麦脱落酸不敏感基因序列分析

doi:10.3969/j.issn.1004-1524.2017.02.02

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (2): 186-192.DOI: 10.3969/j.issn.1004-1524.2017.02.02

荞麦脱落酸不敏感基因序列分析

汪燕¹, 刘瑶², 梁成刚^{1, *}, 陈晴晴¹, 石桃雄¹, 陈其皎¹, 孟子烨¹, 陈庆富¹

1.贵州师范大学荞麦产业技术研究中心,贵州贵阳550001;
2.陕西米仓山国家级自然保护区管理局,陕西汉中 723500

收稿日期:2016-07-29 出版日期:2017-02-15 发布日期:2017-03-06
通讯作者: 梁成刚,E-mail:jesselcg@163.com
作者简介:汪燕(1985—),女,重庆人,博士,助理研究员,从事作物遗传育种与分子机制等相关研究。E-mail:yanwanguf@163.com
基金资助:
贵州省科技厅合作计划项目(黔科合LH字[2015]7770号); 贵州师范大学资助博士科研项目(0516029); 贵州省“三区”人才工作专项经费(0515075); 国家自然科学基金面上项目(31471562); 国家燕麦荞麦现代农业产业技术体系专项资金(CARS-08-A4); 贵州省荞麦工程技术研究中心项目(黔科合农G字[2015]4003号)

Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum

WANG Yan¹, LIU Yao², LIANG Chenggang^{1, *}, CHEN Qingqing¹, SHI Taoxiong¹, CHEN Qijiao¹, MENG Ziye¹, CHEN Qingfu¹

1. Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China;
2. Shaanxi Micangshan National Nature Reserve, Hanzhong 723500, China

Received:2016-07-29 Online:2017-02-15 Published:2017-03-06

摘要/Abstract

摘要： 脱落酸不敏感基因(ABA-insensitive, ABI)是调控植物逆境胁迫应答、种子脱落和休眠等的一类转录因子。生物信息学分析发现,普通荞麦ABI(CL38501)基因cDNA全长为963 bp,编码320个氨基酸,含有典型的bZIP结构域和ABI5家族保守区Ⅱ,推测该基因可能编码ABI5家族蛋白。与野生种质相比,花蕾期和开花期栽培品种ABA信号转导途径中绝大多数基因表达量上调,其中,ABI基因表达量分别上调4.42和3.66倍。对9个栽培品种和5份野生种质的ABI基因进行PCR扩增,发现14份材料的405个位点中多态位点为30个,9个栽培品种间仅含1个多态位点,5个野生种质间含有7个多态位点,说明普通荞麦ABI基因序列高度保守。栽培品种与野生种质ABI序列存在11个共同氨基酸差异位点,蛋白构象预测发现第32位点和第60位点氨基酸发生置换导致蛋白构象发生变化,表明其可能与栽培驯化过程中荞麦丧失休眠与落粒特性相关。

关键词: 普通荞麦, 脱落酸不敏感基因, 序列分析, 蛋白结构

Abstract: ABA-insensitive (ABI) is a kind of transcription factors that regulates plant stress response, seed shattering and dormancy. Bioinformatic analysis indicated that the cDNA sequence of common buckwheat ABI(CL38501) gene contained 963 bp, coded 320 amino acids including the bZIP domain and the conserved domain Ⅱ of ABI5, suggesting that it might encode the ABI5 family protein. The major genes involve in ABA signal transduction pathway were up-regulated in cultivar including ABI that was up-regulated by 4.42-fold and 3.66-fold at bud stage and flowering stage, respectively, as compared with the wild germplasm of common buckwheat. A total of 405 sites in ABI gene including 30 polymorphic sites were identified among 9 cultivars and 5 wild germplasms of common buckwheat. Only 1 polymorphic site was identified among 9 cultivars, whereas 7 polymorphic sites were identified in 5 wild germplasms. These results indicated that the ABI sequence of common buckwheat was highly conserved. A total of 11 consistent polymorphic amino acid sites were identified among cultivar and wild germplasm. The change of protein conformation was found between cultivar and wild germplasm of common buckwheat induced by the amino acid transformation at the 32nd and 60th sites of ABI, implying that it might be associated with the loss of seed shattering and dormancy during the long domestication process.

Key words: common buckwheat, ABA-insensitive gene, sequence analysis, protein structure

中图分类号:

汪燕, 刘瑶, 梁成刚, 陈晴晴, 石桃雄, 陈其皎, 孟子烨, 陈庆富. 荞麦脱落酸不敏感基因序列分析[J]. 浙江农业学报, 2017, 29(2): 186-192.

WANG Yan, LIU Yao, LIANG Chenggang, CHEN Qingqing, SHI Taoxiong, CHEN Qijiao, MENG Ziye, CHEN Qingfu. Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum[J]. , 2017, 29(2): 186-192.

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荞麦脱落酸不敏感基因序列分析

Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum

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