›› 2009, Vol. 21 ›› Issue (3): 0-237.

• 论文 •    

彩色马蹄莲组培快繁体系优化

黄普乐 1,2,孙崇波 2,谢 鸣 2   

  1. 1 浙江省农业科学院综合服务中心,浙江杭州310021;2 浙江省农业科学院园艺研究所,浙江杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25

Optimized rapid propagation system in tissue culture of Zantedeschia hybrida

HUANG Pu-lei;;SUN Chong-bo;XIE Ming   

  1. 1 Combined Service Center,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;2 Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 用0.1%的HgC12对彩色马蹄莲外植体进行常规灭菌,一年生种球的块茎芽灭菌时间12 min对于提高初代培养成活率的效果最好,二年生和三年生种球的块茎芽的最佳灭菌时间以16 min效果最好;在MS培养基上,BA 2.0+GA3 0.3激素配比组合可以比较稳定地诱导出较高5.3倍的增殖倍数,且不定芽生长良好,是最佳激素和浓度配比;采用1/2 MS培养基,IBA浓度为0.5 mg/L,蔗糖浓度为1O g/L时,壮苗生根的效果好;室外炼苗3 d有利于彩色马蹄莲组培苗瓶苗移栽成活,80 d后组培苗成活率达88% ,试验过程中组培苗瓶苗在3月10 13移栽成活率最高,达到100%。

关键词: 彩色马蹄莲, 组培, 快繁

Abstract: The explants of Zantedeschia hybrida were disinfected by 0.1% HgCl2.The results showed that 12 min was the best sterilization time for annual tuber bud while 16min was the best steirlization time for biennial and three-year-old tuber
bud.The best hormone combination was BA 2.0 mg/L+GA3 0.3 mg/L for MS medium,which could induce a higher proliferation multiple of adventitious buds of5.3.Meanwhile,the adventitious buds grew well.The suitable growing medium for plantlet rooting was 1/2 MS medium+ 0.5 mg/L IBA + 10 g/L sucrose.It was benefit to increase the transplantation survival rate when the plantlets were treated with 3 d outdoor acclimation.When transplanted after 80 d,the survival rate of plantlets was 88%.In this experiment,March 10 was the best transplanting date,on which the survival rate of plantlets achieved 100%.

Key words: Zantedeschia hybrida, tissue culture, rapid propagation