台湾独蒜兰组培繁育及其假鳞茎的化学组成分析

doi:10.3969/j.issn.1004-1524.20230370

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (2): 358-364.DOI: 10.3969/j.issn.1004-1524.20230370

台湾独蒜兰组培繁育及其假鳞茎的化学组成分析

孙健(), 任江剑^*(), 王海阁, 江建铭, 王志安, 俞旭平

浙江省中药研究所有限公司,浙江杭州 310023

收稿日期:2023-03-20 出版日期:2024-02-25 发布日期:2024-03-05
作者简介:孙健(1988—),男,山东莱州人,博士,高级工程师,主要从事中药材遗传育种研究。E-mail:jian_sun@zju.edu.cn
通讯作者: *任江剑,E-mail:6936066@qq.com
基金资助:
浙江省重点研发计划(2019C02026);浙江省中药材新品种选育重大科技专项(2021C02074);国家现代农业产业技术体系(CARS-21);浙江省特色中药资源保护与创新利用重点实验室(2021E10013);浙江省药用植物种质改良与质量控制技术重点实验室(2011E10015)

Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs

SUN Jian(), REN Jiangjian^*(), WANG Haige, JIANG Jianming, WANG Zhi’an, YU Xuping

Zhejiang Research Institute of Traditional Chinese Medicine Co., Ltd., Hangzhou 310023, China

Received:2023-03-20 Online:2024-02-25 Published:2024-03-05

摘要/Abstract

摘要：

为构建台湾独蒜兰组培繁育技术体系,分析栽培台湾独蒜兰假鳞茎化学组成特征,经过种子萌发、原球状茎增长、假鳞茎增殖、生根壮苗培养获得台湾独蒜兰植株,以组培台湾独蒜兰植株的假鳞茎为材料,应用非靶向代谢组和HPLC指纹图谱分析假鳞茎化学成分组成。结果表明,经过2年的繁育,单个台湾独蒜兰蒴果可以获得假鳞茎0.5 g左右的幼苗超过1 000株。通过非靶向代谢组数据分析鉴定出不同材料的共有的化合物1 229种,包括羧酸及其衍生物236种、苯及取代衍生物110种、有机氧化合物106种、脂肪酰基化合物94种、黄酮类化合物43种、甾体及甾体衍生物42种,其中以化合物1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯为代表的苄酯类为兰科植物富集化合物。应用HPLC法,测得假鳞茎的1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯含量为0.53%。综上,应用组培技术可以繁育得到台湾独蒜兰的植株,台湾独蒜兰的假鳞茎与独蒜兰一样含有丰富的苄酯类化合物成分。

关键词: 台湾独蒜兰, 组培繁育, 化学成分

Abstract:

The aim of this study was to construct the tissue culture and breeding technology system of Pleione formosana Hayata, and analyze the chemical composition characteristics of pseudobulbs of P. formosana Hayata. After seed germination, protocorm growth, pseudobulb proliferation, rooting and seedling culture, the plants of P. formosana Hayata were obtained. The pseudobulbs of tissue-cultured P. formosana Hayata plants were used as materials. The chemical composition of pseudobulbs was analyzed by non-targeted metabolomics and HPLC fingerprint. After 2 years of breeding, more than 1 000 seedlings with about 0.5 g pseudobulbs were obtained from a single capsule. Through the analysis of non-targeted metabolomics data, 1 229 common compounds were identified in different materials, including 236 carboxylic acids and their derivatives, 110 benzene and substituted derivatives, 106 organic oxygen compounds, 94 fatty acyl compounds, 43 flavonoids, and 42 steroids and steroid derivatives. Among them, the benzyl esters represented by 1,4-bis [4-(glucoseoxy) benzyl]-2-isobutylmalate are orchid-enriched compounds. Meanwhile the content of 1,4-bis [4-(glucoxy ) benzyl]-2-isobutylmalate was 0.53% in pseudobulbs detected by HPLC. The plants of P. formosana Hayata can be bred by tissue culture technology. And the pseudobulbs of P. formosana Hayata are rich in benzyl ester compounds as well as P. bulbocodioides(Franch.) Rolfe.

Key words: Pleione formosana Hayata, tissue culture breeding, chemical composition

中图分类号:

S682.31

孙健, 任江剑, 王海阁, 江建铭, 王志安, 俞旭平. 台湾独蒜兰组培繁育及其假鳞茎的化学组成分析[J]. 浙江农业学报, 2024, 36(2): 358-364.

SUN Jian, REN Jiangjian, WANG Haige, JIANG Jianming, WANG Zhi’an, YU Xuping. Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 358-364.

图/表 4

参考文献 15

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激素处理 Hormone treatment/(mg·L^-1)	增殖倍数 Proliferation rate	球状茎形态 Spherical bulbous	球状茎质量 Bulbous weight/g
6-BA 1.0+NAA 0.2	1.3±0.2 e	细小Slender	0.06±0.01 d
6-BA 1.5+NAA 0.2	2.6±0.4 d	细小Slender	0.08±0.01 c
6-BA 2.0+NAA 0.2	3.4±0.3 c	中等Medium	0.10±0.01 b
6-BA 2.5+NAA 0.2	5.7±0.3 a	粗壮Thick	0.13±0.02 a
6-BA 3.0+NAA 0.2	4.3±0.5 bc	中等Medium	0.11±0.02 ab
6-BA 3.5+NAA 0.2	3.8±0.4 c	中等Medium	0.09±0.02 b

激素处理 Hormone treatment/(mg·L^-1)	增殖倍数 Proliferation rate	球状茎形态 Spherical bulbous	球状茎质量 Bulbous weight/g
6-BA 1.0+NAA 0.2	1.3±0.2 e	细小Slender	0.06±0.01 d
6-BA 1.5+NAA 0.2	2.6±0.4 d	细小Slender	0.08±0.01 c
6-BA 2.0+NAA 0.2	3.4±0.3 c	中等Medium	0.10±0.01 b
6-BA 2.5+NAA 0.2	5.7±0.3 a	粗壮Thick	0.13±0.02 a
6-BA 3.0+NAA 0.2	4.3±0.5 bc	中等Medium	0.11±0.02 ab
6-BA 3.5+NAA 0.2	3.8±0.4 c	中等Medium	0.09±0.02 b

激素处理 Hormone treatment/(mg·L^-1)	生根率 Rooting rate/%	幼苗形态 Seedling morphology	假鳞茎质量 Pseudobulb weight/g
NAA 0.2+6-BA 0.2+IBA 1.0+2.4-D 0.01	83.0±6.0 b	中等Medium	0.41±0.06 b
NAA 0.3+6-BA 0.3+IBA 1.0+2.4-D 0.01	99.0±1.0 a	纤细Slender	0.31±0.02 c
NAA 0.5+6-BA 0.5+IBA 1.0+2.4-D 0.01	100.0±0.0a	粗壮Thick	0.52±0.05 a
NAA 0.8+6-BA 0.8+IBA 1.0+2.4-D 0.01	95.0±4.0 ab	中等Medium	0.37±0.04 b
NAA 1.0+6-BA 1.0+IBA 1.0+2.4-D 0.01	87.0±5.0 b	粗壮Thick	0.48±0.05 a

激素处理 Hormone treatment/(mg·L^-1)	生根率 Rooting rate/%	幼苗形态 Seedling morphology	假鳞茎质量 Pseudobulb weight/g
NAA 0.2+6-BA 0.2+IBA 1.0+2.4-D 0.01	83.0±6.0 b	中等Medium	0.41±0.06 b
NAA 0.3+6-BA 0.3+IBA 1.0+2.4-D 0.01	99.0±1.0 a	纤细Slender	0.31±0.02 c
NAA 0.5+6-BA 0.5+IBA 1.0+2.4-D 0.01	100.0±0.0a	粗壮Thick	0.52±0.05 a
NAA 0.8+6-BA 0.8+IBA 1.0+2.4-D 0.01	95.0±4.0 ab	中等Medium	0.37±0.04 b
NAA 1.0+6-BA 1.0+IBA 1.0+2.4-D 0.01	87.0±5.0 b	粗壮Thick	0.48±0.05 a

台湾独蒜兰组培繁育及其假鳞茎的化学组成分析

Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs

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