›› 2013, Vol. 25 ›› Issue (2): 0-234.

• 论文 •    

热休克蛋白70基因(HSP70)部分片段的单核苷酸多态性及其与蛋鸭产蛋性能的关联性

曾涛1,2,李国勤1,王德前1,李进军1,田勇1,陈黎1,黄爱霞1,石放雄2,*,沈军达1,
陶争荣1,卢立志1,*
  

  1. 1浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021;2南京农业大学 动物科技学院,江苏 南京 210095
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-25 发布日期:2013-03-25

Single nucleotide polymorphisms (SNPs) of HSP70 gene segments and their association with laying performance of domestic duck

ZENG Tao;LI Guo-qin;WANG De-qian;LI Jin-jun;TIAN Yong;CHEN Li;HUANG Ai-xia;SHI Fang-xiong;*;SHEN Jun-da;TAO Zheng-rong;LU Li-zhi;*   

  1. 1Institute of Animal Husbandry & Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;2College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-25 Published:2013-03-25

摘要: 研究旨在搜寻热休克蛋白70(HSP70)基因的突变位点,分析蛋鸭抗热应激分子育种潜在的遗传标记。以缙云麻鸭为研究对象,用PCR方法扩增了蛋鸭HSP70基因的部分片段,采用直接测序的方法检测该片段的多态性,同时用最小二乘法分析其与夏季笼养蛋鸭产蛋性能的关联性。结果在HSP70基因部分片段中发现1476(C/T)和1596(C/T) 2个SNP位点。其中1476(C/T)位点的CT基因型占70.00%,TT基因型占16.67%,CC基因型占13.33%;1596(C/T)位点的CT基因型占48.33%,TT基因型占41.67%,CC基因型占10.00%。关联分析结果显示,1476(C/T)位点不同基因型在产蛋量上存在差异,其中CC基因型个体的产蛋量显著高于CT基因型和TT基因型(P<0.05);C1596T位点不同基因型在产蛋量上无明显差异(P>0.05)。由于样本量较少,故有必要进一步加大样本量进行研究.

关键词: 蛋鸭, 单核苷酸多态性, HSP70, 产蛋性能

Abstract: The research aimed to seek the mutation sites of HSP70 gene and find the quantitative trait locus which provided a basis for molecular marker-assisted selection. Sixty Jinyun ducks were used as the experimental animals. Partial fragments of duck HSP70 gene were amplified by PCR method. The polymorphisms of the target fragment were detected by the methods of sequencing, and then the least square mean and genetic variance of different genotypes at polymorphic loci were analyzed. The results showed that there were two SNPs (1476(C/T) and 1596(C/T)) in HSP70 gene. The frequencies of CT, TT and CC were 70.00%, 16.67% and 1333% at the C1476T site, but 48.33%, 41.67% and 10.00% at C1596T site, respectively. Association analysis showed that the egg production of genotype CC was significantly higher than genotype CT and genotype TT for laying performance in 1476(C/T) locus (P<0.05). The 1596(C/T) site showed no significant correlation with laying performance (P>0.05). The results provided a preliminary theoretical data for molecular assisted breeding of laying duck, but because of the sample was small, it is necessary to increase the sample size for further research and analysis.

Key words: domestic duck, single nucleotide polymorphisms (SNP), HSP70, laying performance