›› 2014, Vol. 26 ›› Issue (2): 0-279284.

• 作物科学 •    

二年残孔菌胞外多糖组分分析及其在卷烟中的应用

曹健1,2,张惠杰1,许春平3,*   

  1. 1河南工业大学 生物工程学院,河南 郑州450001;2中原工学院,河南 郑州 450007;3郑州轻工业学院,河南 郑州 450000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-03-25 发布日期:2014-07-09

Component analysis of extracellular polysaccharide by Abortiporus biennis and its application in cigarette aroma

CAO Jian;ZHANG Huijie;XU Chunping;*   

  1. 1School of Bioengineering, Henan University of Technology, Zhengzhou 450001, China; 2Zhongyuan University of Technology, Zhengzhou 450007, China; 3Zhengzhou University of Light Industry, Zhengzhou 450000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-03-25 Published:2014-07-09

摘要: 为开发新型天然菌物香料,利用Sepharose CL6B凝胶柱对二年残孔菌胞外多糖(EPS)进行分离纯化,并利用红外光谱(IR)、气质联用仪(GC/MS)对EPS的特征性结构和单糖组分进行分析。同时,将EPS进行烟草薄片加香实验,采用同时蒸馏萃取和GC/MS分析了试验组与空白组卷烟中香味物质的变化。结果表明,二年残孔菌EPS为单组分多糖,主要单糖组成为葡萄糖5145%,甘露糖3030%,半乳糖1551%。GC/MS分析结果显示,将EPS用于制烟后,烟叶中酮类、醛类和呋喃类的含量增加,酚类、氮杂环类的含量显著下降,这有利于减轻烟气刺激性、改善和修饰卷烟香气,进而提高烟气整体品质。

关键词: 二年残孔菌, 发酵, 胞外多糖, 香气成分

Abstract: In order to develop new natural fungal perfume, the fermented extracellular polysaccharides (EPS) from Abortiporus biennis were purified by Sepharose CL6B. The structure and monosaccharide composition of EPS were investigated by infrared spectroscopy (IR) and GCMS analysis. After addition of EPS into tobacco conduction, the changes of flavoring substances in cigarettes were compared with control by simultaneous distillation and GC/MS. It was shown that EPS was monocomponent with the monosaccharide composition of 5145% glucose, 3030% mannose and 1551% galactose. With application of EPS in tobacco production, the contents of ketones, aldehydes and furans increased in the cigarettes, while the contents of phenols and heterocyclic compounds decreased significantly. The treatments with EPS could reduce stimulation of smoking, and improve the aroma of cigarette to ameliorate the quality of smoking.

Key words: Abortiporus biennis, fermentation, extracellular polysaccharides, aroma components