浙江农业学报 ›› 2024, Vol. 36 ›› Issue (6): 1245-1255.DOI: 10.3969/j.issn.1004-1524.20230204
桑树桑黄JM-1胞外多糖液态培养基优化及其抗氧化性研究
曹乃馨1,2(
), 罗阳兰1,*(), 阎勇1, 解修超2, 张雯龙1
- 1.广西壮族自治区农业科学院 微生物研究所,广西 南宁 530007
2.陕西理工大学 生物科学与工程学院,陕西 汉中 723000
-
收稿日期:2023-02-22出版日期:2024-06-25发布日期:2024-07-02 -
作者简介:曹乃馨(1996—),女,陕西西安人,硕士研究生,研究方向为微生物代谢活性物质。E-mail:2629248365@qq.com -
通讯作者:*罗阳兰,E-mail:595059345@qq.com -
基金资助:广西农业科学院科技发展基金(桂农科2021JM85);广西农业科学院科技发展基金(桂农科2022JM07);创新联盟专项—科技先锋队“强农富民”“六个一”专项行动(桂农科盟202308)
Optimization and antioxidant activity of liquid medium of extracellular polysaccharides from Sanghuangporus sanghuang JM-1
CAO Naixin1,2(), LUO Yanglan1,*(), YAN Yong1, XIE Xiuchao2, ZHANG Wenlong1
- 1. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
2. School of Biological Science and Technology, Shaanxi University of Technology, Hanzhong 723000, Shaanxi, China
-
Received:2023-02-22Online:2024-06-25Published:2024-07-02
摘要：
为优化桑树桑黄胞外多糖液态发酵培养基配方并探究其体外抗氧化活性,利用组织分离法纯化并鉴定1株桑树桑黄菌株JM-1,通过Box-Behnken响应面法优化菌株胞外多糖液态培养基配方,并考察其体外抗氧化活性。结果表明,优化后的液体培养基配方为玉米粉5.4 g·L-1、葡萄糖17.0 g·L-1、蛋白胨5.8 g·L-1、维生素B1(VB1),10.0 mg·L-1、KH2PO4 3.0 g·L-1、MgSO4·7H2O 5.0 g·L-1,该条件下菌株JM-1产胞外多糖含量为5.918 g·L-1,比基础培养基提高了13.63%。抗氧化活性试验结果表明,在质量浓度为5 mg·mL-1时,JM-1菌株产胞外多糖的还原能力为3.187 mmol·L-1,对DPPH自由基和羟自由基的清除率分别为63.9%、54.3%。研究结果可为桑黄资源的开发利用提供科学参考。
中图分类号:
引用本文
曹乃馨, 罗阳兰, 阎勇, 解修超, 张雯龙. 桑树桑黄JM-1胞外多糖液态培养基优化及其抗氧化性研究[J]. 浙江农业学报, 2024, 36(6): 1245-1255.
CAO Naixin, LUO Yanglan, YAN Yong, XIE Xiuchao, ZHANG Wenlong. Optimization and antioxidant activity of liquid medium of extracellular polysaccharides from Sanghuangporus sanghuang JM-1[J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1245-1255.
表1 Plackett-Burman设计的因素与水平
Table 1 Factors and levels of Plackett-Burman design
| 因素 Factor | 不同编码水平的实际添加量 Addition amount under different coding levels | |
|---|---|---|
| -1 | 1 | |
| (A)玉米粉Cornmeal/(g·L-1) | 10 | 20 |
| (B)葡萄糖Glucose/(g·L-1) | 5 | 10 |
| (C)蛋白胨Peptone/(g·L-1) | 3 | 5 |
| (D)维生素B1 Vitamin B1/(mg·L-1) | 10 | 15 |
| (E)KH2PO4/(g·L-1) | 3 | 5 |
| (F)MgSO4·7H2O/(g·L-1) | 3 | 5 |
表1 Plackett-Burman设计的因素与水平
Table 1 Factors and levels of Plackett-Burman design
| 因素 Factor | 不同编码水平的实际添加量 Addition amount under different coding levels | |
|---|---|---|
| -1 | 1 | |
| (A)玉米粉Cornmeal/(g·L-1) | 10 | 20 |
| (B)葡萄糖Glucose/(g·L-1) | 5 | 10 |
| (C)蛋白胨Peptone/(g·L-1) | 3 | 5 |
| (D)维生素B1 Vitamin B1/(mg·L-1) | 10 | 15 |
| (E)KH2PO4/(g·L-1) | 3 | 5 |
| (F)MgSO4·7H2O/(g·L-1) | 3 | 5 |
表2 响应面试验的因素与水平
Table 2 Factors and levels of response surface experiment
| 因素 Factor | 不同编码水平的实际添加量 Addition amount under different coding levels | ||
|---|---|---|---|
| -1 | 0 | 1 | |
| (A)玉米粉Cornmeal/(g·L-1) | 5.0 | 7.5 | 10.0 |
| (B)葡萄糖Glucose/(g·L-1) | 12.5 | 15.0 | 17.5 |
| (C)蛋白胨Peptone/(g·L-1) | 5.0 | 5.5 | 6.0 |
表2 响应面试验的因素与水平
Table 2 Factors and levels of response surface experiment
| 因素 Factor | 不同编码水平的实际添加量 Addition amount under different coding levels | ||
|---|---|---|---|
| -1 | 0 | 1 | |
| (A)玉米粉Cornmeal/(g·L-1) | 5.0 | 7.5 | 10.0 |
| (B)葡萄糖Glucose/(g·L-1) | 12.5 | 15.0 | 17.5 |
| (C)蛋白胨Peptone/(g·L-1) | 5.0 | 5.5 | 6.0 |
图1 菌株JM-1的形态特征
Fig.1 Morphological characteristics of JM-1 strain
图1 菌株JM-1的形态特征
Fig.1 Morphological characteristics of JM-1 strain
图2 基于JM-1菌株ITS序列的系统发育树
Fig.2 Phylogenetic tree of strain JM-1 based on ITS sequences
图2 基于JM-1菌株ITS序列的系统发育树
Fig.2 Phylogenetic tree of strain JM-1 based on ITS sequences
图3 不同碳源对胞外多糖含量的影响 柱上无相同字母的表示差异显著(P<0.05)。下同。
Fig.3 Effect of carbon sources on extracellular polysaccharide content Bars marked without the same letters indicate significant difference at P<0.05. The same as below.
图3 不同碳源对胞外多糖含量的影响 柱上无相同字母的表示差异显著(P<0.05)。下同。
Fig.3 Effect of carbon sources on extracellular polysaccharide content Bars marked without the same letters indicate significant difference at P<0.05. The same as below.
图4 不同氮源对胞外多糖含量的影响
Fig.4 Effect of nitrogen sources on extracellular polysaccharide content
图4 不同氮源对胞外多糖含量的影响
Fig.4 Effect of nitrogen sources on extracellular polysaccharide content
图5 不同生长因子对胞外多糖含量的影响
Fig.5 Effect of growth factors on extracellular polysaccharide content
图5 不同生长因子对胞外多糖含量的影响
Fig.5 Effect of growth factors on extracellular polysaccharide content
图6 不同无机盐对胞外多糖含量的影响
Fig.6 Effect of inorganic salts on extracellular polysaccharide content
图6 不同无机盐对胞外多糖含量的影响
Fig.6 Effect of inorganic salts on extracellular polysaccharide content
表3 Plackett-Burman试验的设计方案与结果
Table 3 Design and results of Plackett-Burman experiment
| 序号 No. | 各因素的编码水平Coding level of factors | 胞外多糖含量 Extracellular polysaccharide content/(g·L-1) | |||||
|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | ||
| 1 | 1 | -1 | 1 | -1 | -1 | -1 | 4.449 |
| 2 | 1 | 1 | -1 | 1 | -1 | -1 | 4.827 |
| 3 | -1 | 1 | 1 | -1 | 1 | -1 | 5.486 |
| 4 | 1 | -1 | 1 | 1 | -1 | 1 | 4.385 |
| 5 | 1 | 1 | -1 | 1 | 1 | -1 | 3.964 |
| 6 | 1 | 1 | 1 | -1 | 1 | 1 | 4.934 |
| 7 | -1 | 1 | 1 | 1 | -1 | 1 | 5.538 |
| 8 | -1 | -1 | 1 | 1 | 1 | -1 | 4.781 |
| 9 | -1 | -1 | -1 | 1 | 1 | 1 | 4.278 |
| 10 | 1 | -1 | -1 | -1 | 1 | 1 | 3.906 |
| 11 | -1 | 1 | -1 | -1 | -1 | 1 | 4.956 |
| 12 | -1 | -1 | -1 | -1 | -1 | -1 | 4.230 |
表3 Plackett-Burman试验的设计方案与结果
Table 3 Design and results of Plackett-Burman experiment
| 序号 No. | 各因素的编码水平Coding level of factors | 胞外多糖含量 Extracellular polysaccharide content/(g·L-1) | |||||
|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | ||
| 1 | 1 | -1 | 1 | -1 | -1 | -1 | 4.449 |
| 2 | 1 | 1 | -1 | 1 | -1 | -1 | 4.827 |
| 3 | -1 | 1 | 1 | -1 | 1 | -1 | 5.486 |
| 4 | 1 | -1 | 1 | 1 | -1 | 1 | 4.385 |
| 5 | 1 | 1 | -1 | 1 | 1 | -1 | 3.964 |
| 6 | 1 | 1 | 1 | -1 | 1 | 1 | 4.934 |
| 7 | -1 | 1 | 1 | 1 | -1 | 1 | 5.538 |
| 8 | -1 | -1 | 1 | 1 | 1 | -1 | 4.781 |
| 9 | -1 | -1 | -1 | 1 | 1 | 1 | 4.278 |
| 10 | 1 | -1 | -1 | -1 | 1 | 1 | 3.906 |
| 11 | -1 | 1 | -1 | -1 | -1 | 1 | 4.956 |
| 12 | -1 | -1 | -1 | -1 | -1 | -1 | 4.230 |
表4 Plackett-Burman试验结果分析
Table 4 Analysis of Plackett-Burman experiment results
| 项 Term | 效应 Effect | 系数 Coefficient | 系数标准误 Standard error of coefficient | t值 t value | P值 P value |
|---|---|---|---|---|---|
| 常量Constant | — | 4.644 5 | 0.0719 | 64.62 | <0.001 |
| A | -0.467 3 | -0.233 7 | 0.0719 | -3.25 | 0.023 |
| B | 0.612 7 | 0.306 3 | 0.0719 | 4.26 | 0.008 |
| C | 0.568 7 | 0.284 3 | 0.0719 | 3.96 | 0.011 |
| D | -0.031 3 | -0.0157 | 0.0719 | -0.22 | 0.836 |
| E | -0.172 7 | -0.086 3 | 0.0719 | -1.20 | 0.283 |
| F | 0.043 3 | 0.021 7 | 0.0719 | 0.30 | 0.775 |
表4 Plackett-Burman试验结果分析
Table 4 Analysis of Plackett-Burman experiment results
| 项 Term | 效应 Effect | 系数 Coefficient | 系数标准误 Standard error of coefficient | t值 t value | P值 P value |
|---|---|---|---|---|---|
| 常量Constant | — | 4.644 5 | 0.0719 | 64.62 | <0.001 |
| A | -0.467 3 | -0.233 7 | 0.0719 | -3.25 | 0.023 |
| B | 0.612 7 | 0.306 3 | 0.0719 | 4.26 | 0.008 |
| C | 0.568 7 | 0.284 3 | 0.0719 | 3.96 | 0.011 |
| D | -0.031 3 | -0.0157 | 0.0719 | -0.22 | 0.836 |
| E | -0.172 7 | -0.086 3 | 0.0719 | -1.20 | 0.283 |
| F | 0.043 3 | 0.021 7 | 0.0719 | 0.30 | 0.775 |
表5 最陡爬坡试验的设计与结果
Table 5 Design and results of the steepest ascent test g·L-1
| 序号 No. | 添加量Addition amount | 胞外多糖含量 Extracellular polysaccharide content | ||
|---|---|---|---|---|
| 玉米粉 Cornmeal | 葡萄糖 Glucose | 蛋白胨 Peptone | ||
| 1 | 15.0 | 7.5 | 4.0 | 4.788 |
| 2 | 12.5 | 10.0 | 4.5 | 4.972 |
| 3 | 10.0 | 12.5 | 5.0 | 5.623 |
| 4 | 7.5 | 15.0 | 5.5 | 5.861 |
| 5 | 5.0 | 17.5 | 6.0 | 5.309 |
| 6 | 2.5 | 20.0 | 6.5 | 4.834 |
表5 最陡爬坡试验的设计与结果
Table 5 Design and results of the steepest ascent test g·L-1
| 序号 No. | 添加量Addition amount | 胞外多糖含量 Extracellular polysaccharide content | ||
|---|---|---|---|---|
| 玉米粉 Cornmeal | 葡萄糖 Glucose | 蛋白胨 Peptone | ||
| 1 | 15.0 | 7.5 | 4.0 | 4.788 |
| 2 | 12.5 | 10.0 | 4.5 | 4.972 |
| 3 | 10.0 | 12.5 | 5.0 | 5.623 |
| 4 | 7.5 | 15.0 | 5.5 | 5.861 |
| 5 | 5.0 | 17.5 | 6.0 | 5.309 |
| 6 | 2.5 | 20.0 | 6.5 | 4.834 |
表6 响应面试验方案与结果
Table 6 Design and results of response surface test
| 试验号 Test No. | 各因素的编码水平 Coding levels of factors | 胞外多糖含量 Extracellular polysaccharide content/(g·L-1) | ||
|---|---|---|---|---|
| A | B | C | ||
| 1 | -1 | -1 | 0 | 5.632 |
| 2 | 1 | -1 | 0 | 5.591 |
| 3 | -1 | 1 | 0 | 5.932 |
| 4 | 1 | 1 | 0 | 5.684 |
| 5 | -1 | 0 | -1 | 5.589 |
| 6 | 1 | 0 | -1 | 5.603 |
| 7 | -1 | 0 | 1 | 5.875 |
| 8 | 1 | 0 | 1 | 5.635 |
| 9 | 0 | -1 | -1 | 5.568 |
| 10 | 0 | 1 | -1 | 5.681 |
| 11 | 0 | -1 | 1 | 5.654 |
| 12 | 0 | 1 | 1 | 5.815 |
| 13 | 0 | 0 | 0 | 5.907 |
| 14 | 0 | 0 | 0 | 5.888 |
| 15 | 0 | 0 | 0 | 5.876 |
| 16 | 0 | 0 | 0 | 5.852 |
| 17 | 0 | 0 | 0 | 5.859 |
表6 响应面试验方案与结果
Table 6 Design and results of response surface test
| 试验号 Test No. | 各因素的编码水平 Coding levels of factors | 胞外多糖含量 Extracellular polysaccharide content/(g·L-1) | ||
|---|---|---|---|---|
| A | B | C | ||
| 1 | -1 | -1 | 0 | 5.632 |
| 2 | 1 | -1 | 0 | 5.591 |
| 3 | -1 | 1 | 0 | 5.932 |
| 4 | 1 | 1 | 0 | 5.684 |
| 5 | -1 | 0 | -1 | 5.589 |
| 6 | 1 | 0 | -1 | 5.603 |
| 7 | -1 | 0 | 1 | 5.875 |
| 8 | 1 | 0 | 1 | 5.635 |
| 9 | 0 | -1 | -1 | 5.568 |
| 10 | 0 | 1 | -1 | 5.681 |
| 11 | 0 | -1 | 1 | 5.654 |
| 12 | 0 | 1 | 1 | 5.815 |
| 13 | 0 | 0 | 0 | 5.907 |
| 14 | 0 | 0 | 0 | 5.888 |
| 15 | 0 | 0 | 0 | 5.876 |
| 16 | 0 | 0 | 0 | 5.852 |
| 17 | 0 | 0 | 0 | 5.859 |
图7 不同因素交互的响应曲面
Fig.7 Response surface plots of different factors
图7 不同因素交互的响应曲面
Fig.7 Response surface plots of different factors
图8 胞外多糖的总抗氧化能力
Fig.8 Total antioxidant capacity of extracellular polysaccharides
图8 胞外多糖的总抗氧化能力
Fig.8 Total antioxidant capacity of extracellular polysaccharides
图9 胞外多糖对DPPH自由基的清除能力
Fig.9 DPPH free radical scavenging ability of extracellular polysaccharides
图9 胞外多糖对DPPH自由基的清除能力
Fig.9 DPPH free radical scavenging ability of extracellular polysaccharides
图10 胞外多糖对羟自由基的清除能力
Fig.10 Hydroxyl radical scavenging ability of extracellular polysaccharides
图10 胞外多糖对羟自由基的清除能力
Fig.10 Hydroxyl radical scavenging ability of extracellular polysaccharides
| [1] | WU F, ZHOU L W, VLASÁK J, et al. Global diversity and systematics of Hymenochaetaceae with poroid hymenophore[J]. Fungal Diversity, 2022, 113(1): 1-192. |
| [2] | 戴玉成, 崔宝凯. 药用真菌桑黄种类研究[J]. 北京林业大学学报, 2014, 36(5): 1-7. |
| DAI Y C, CUI B K. Progress on the species of medicinal fungus Inonotus sanghuang[J]. Journal of Beijing Forestry University, 2014, 36(5): 1-7. (in Chinese with English abstract) | |
| [3] | LIN W C, DENG J S, HUANG S S, et al. Anti-inflammatory activity of Sanghuangporus sanghuang mycelium[J]. International Journal of Molecular Sciences, 2017, 18(2): 347. |
| [4] | 陈万超, 杨焱, 张劲松, 等. 桑黄类真菌活性代谢产物的研究进展[J]. 食用菌学报, 2020, 27(4): 188-201. |
| CHEN W C, YANG Y, ZHANG J S, et al. Recent advances in bioactive metabolites from ‘Sanghuang’ mushrooms[J]. Acta Edulis Fungi, 2020, 27(4): 188-201. (in Chinese with English abstract) | |
| [5] | 史帧婷, 包海鹰. 桑黄类真菌有效成分及功效研究进展[J]. 中国实验方剂学杂志, 2016, 22(22): 197-202. |
| SHI Z T, BAO H Y. Research progress on effective components and efficacy of Phellinus igniarius[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(22): 197-202. (in Chinese with English abstract) | |
| [6] | 朱琳, 崔宝凯. 药用真菌桑黄的研究进展[J]. 菌物研究, 2016, 14(4): 201-209. |
| ZHU L, CUI B K. Progress on the studies of medicinal mushrooms “Sanghuang”group[J]. Journal of Fungal Research, 2016, 14(4): 201-209. (in Chinese with English abstract) | |
| [7] | PARK H J, HAN E S, PARK D K, et al. An extract of Phellinus linteus grown on germinated brown rice inhibits inflammation markers in RAW264.7 macrophages by suppressing inflammatory cytokines, chemokines, and mediators and up-regulating antioxidant activity[J]. Journal of Medicinal Food, 2010, 13(6): 1468-1477. |
| [8] | 宋吉玲, 王伟科, 闫静, 等. 药用真菌桑黄抗氧化物质及其活性研究[J]. 西北农林科技大学学报(自然科学版), 2022, 50(3): 144-154. |
| SONG J L, WANG W K, YAN J, et al. Antioxidant substances and activity of medicinal fungus Sanghuangporus[J]. Journal of Northwest A & F University(Natural Science Edition), 2022, 50(3): 144-154. (in Chinese with English abstract) | |
| [9] | 王豪, 钱坤, 司静, 等. 桑黄类真菌多糖研究进展[J]. 菌物学报, 2021, 40(4): 895-911. |
| WANG H, QIAN K, SI J, et al. Research advances on polysaccharides from sanghuang[J]. Mycosystema, 2021, 40(4): 895-911. (in Chinese with English abstract) | |
| [10] | 黄越, 黄传书, 吴均, 等. 桑资源生物活性物质及其在食品领域应用研究进展[J]. 食品与发酵工业, 2003, 49(23):362-370. |
| HUANG Y, HUANG C S, WU J, et al. Research progress on bioactive substances of mulberry resources and their application in food field[J]. Food and Fermentation Industries, 2003, 49(23):362-370. (in Chinese with English abstract) | |
| [11] | 范祺, 吴茂玉, 张博华, 等. 桑黄的主要活性成分及国内外研究进展[J]. 中国果菜, 2022, 42(11): 50-55. |
| FAN Q, WU M Y, ZHANG B H, et al. Main active components of Phellinus linteus and its domestic and international research progress[J]. China Fruit & Vegetable, 2022, 42(11): 50-55. (in Chinese with English abstract) | |
| [12] | 张健, 侯玉浩, 杜继甫, 等. 桑树桑黄、瓦尼桑黄和粗毛纤孔菌免疫调节作用的比较研究[J]. 菌物学报, 2023, 42(4):907-915. |
| ZHANG J, HOU Y H, DU J F, et al. A comparative study on immunomodulatory effects of water extracts of Inonotus hispidus, Sanghuangporus sanghuang and S. vaninii[J]. Mycosystema, 2023, 42(4):907-915. (in Chinese with English abstract) | |
| [13] | 颜梦秋, 刘艳芳, 周帅, 等. 食药用菌液体发酵及功能活性成分研究现状与展望[J]. 微生物学通报, 2023, 50(7): 3211-3231. |
| YAN M Q, LIU Y F, ZHOU S, et al. Liquid fermentation technology and functional components of edible and medicinal fungi[J]. Microbiology China, 2023, 50(7): 3211-3231. (in Chinese with English abstract) | |
| [14] | 刘军生, 解修超, 罗阳兰, 等. Aspergillus sp. EA-LJS80的分离及其发酵液提取物七叶皂苷C的生物活性[J]. 菌物学报, 2018, 37(1): 79-87. |
| LIU J S, XIE X C, LUO Y L, et al. Escin C from Aspergillus sp. EA-LJS80 and its biological activities[J]. Mycosystema, 2018, 37(1): 79-87. (in Chinese with English abstract) | |
| [15] | 罗阳兰, 邓百万, 刘军生, 等. 秦巴山区蕙兰内生真菌多样性分析[J]. 河南农业科学, 2019, 48(5): 113-121. |
| LUO Y L, DENG B W, LIU J S, et al. Diversity analysis of endophytic fungi isolated from Cymbidium faberi in Qin-Ba Mountains[J]. Journal of Henan Agricultural Sciences, 2019, 48(5): 113-121. (in Chinese with English abstract) | |
| [16] | 张彩妮, 邓百万, 柏秋月, 等. 香菇胞外多糖及生物活性的对比[J]. 北方园艺, 2021(20): 126-131. |
| ZHANG C N, DENG B W, BAI Q Y, et al. Comparative analysis of extracellular polysaccharide and bioactivity associated of Lentinus edodes[J]. Northern Horticulture, 2021(20): 126-131. (in Chinese with English abstract) | |
| [17] | 赵淑杰, 郑丽宁, 路飘飘, 等. Sevag法脱鹿药多糖蛋白工艺条件优化[J]. 中国兽药杂志, 2017, 51(4): 25-29. |
| ZHAO S J, ZHENG L N, LU P P, et al. Study on the orthogonal experiment design of removing protein from polysaccharide of Smilacina japonica[J]. Chinese Journal of Veterinary Drug, 2017, 51(4): 25-29. (in Chinese with English abstract) | |
| [18] | 王欢, 刘明珠, 芦永昌. 青海高山辣根菜中水溶性总多糖的提取工艺研究[J]. 青海农林科技, 2021(4): 13-17. |
| WANG H, LIU M Z, LU Y C. Study on extraction technology of water soluble total polysaccharide from Pegaeophyton scapiflorum in Qinghai[J]. Science and Technology of Qinghai Agriculture and Forestry, 2021(4): 13-17. (in Chinese with English abstract) | |
| [19] | 赵凯, 许鹏举, 谷广烨. 3, 5-二硝基水杨酸比色法测定还原糖含量的研究[J]. 食品科学, 2008, 29(8): 534-536. |
| ZHAO K, XU P J, GU G Y. Study on determination of reducing sugar content using 3, 5-dinitrosalicylic acid method[J]. Food Science, 2008, 29(8): 534-536. (in Chinese with English abstract) | |
| [20] | 梁冬秋, 张翔宇, 张松. 白蜡多年菌液体培养产胞外多糖条件的研究[J]. 广东农业科学, 2015, 42(20): 97-101. |
| LIANG D Q, ZHANG X Y, ZHANG S. Submerged culture conditions for exopolysaccharide production by Perenniporia fraxinea[J]. Guangdong Agricultural Sciences, 2015, 42(20): 97-101. (in Chinese with English abstract) | |
| [21] | 郑宇, 董爱静, 殷海松, 等. 苹果醋发酵营养盐的开发及应用[J]. 中国食品学报, 2015, 15(3): 74-81. |
| ZHENG Y, DONG A J, YIN H S, et al. Development and application of nutrient salts for cider vinegar fermentation[J]. Journal of Chinese Institute of Food Science and Technology, 2015, 15(3): 74-81. (in Chinese with English abstract) | |
| [22] | 王寒, 罗庆华, 魏梦雅, 等. 大鲵油体外抗氧化活性研究[J]. 中国油脂, 2018, 43(9): 149-153. |
| WANG H, LUO Q H, WEI M Y, et al. In vitro antioxidant activity of Chinese giant salamander oil[J]. China Oils and Fats, 2018, 43(9): 149-153. (in Chinese with English abstract) | |
| [23] | 罗阳兰, 邓百万, 刘军生, 等. 白及花与块茎石油醚部位成分分析及其生物活性的比较研究[J]. 中国现代应用药学, 2019, 36(4): 444-450. |
| LUO Y L, DENG B W, LIU J S, et al. Comparative research of bioactivity and analysis of petroleum ether components in flower and tuber of Bletilla striata[J]. Chinese Journal of Modern Applied Pharmacy, 2019, 36(4): 444-450. (in Chinese with English abstract) | |
| [24] | 方荣俊, 曹旭, 程嘉翎, 等. 桑黄的属性考证[J]. 蚕业科学, 2022, 48(2): 141-151. |
| FANG R J, CAO X, CHENG J L, et al. Textual research on attributes of Sanghuang (Sanghuangporus sanghuang) from mulberry tree[J]. Acta Sericologica Sinica, 2022, 48(2): 141-151. (in Chinese with English abstract) | |
| [25] | 寇丹丹, 兰润, 叶伟建, 等. 魔芋葡甘聚糖/普鲁兰多糖半互穿网络水凝胶弹性及其微观形貌研究[J]. 西南大学学报(自然科学版), 2014, 36(4): 205-212. |
| KOU D D, LAN R, YE W J, et al. Study on the semi-interpenetrating polymer network hydrogel springiness of konjac glucomannan and pullulan[J]. Journal of Southwest University(Natural Science Edition), 2014, 36(4): 205-212. (in Chinese with English abstract) | |
| [26] | 王一菲, 于晓丹, 田雪梅, 等. 栎生桑黄和忍冬桑黄液体培养过程中发酵液抗氧化能力[J]. 菌物学报, 2019, 38(6): 938-950. |
| WANG Y F, YU X D, TIAN X M, et al. Antioxidant activities of Sanghuangporus quercicola and S. lonicericola from fermentation broth in liquid cultivation[J]. Mycosystema, 2019, 38(6): 938-950. (in Chinese with English abstract) | |
| [27] | 袁满, 付婷伟, 徐晨, 等. 桑黄子实体多糖提取工艺优化及其抗氧化活性[J]. 食品工业, 2021, 42(11): 103-108. |
| YUAN M, FU T W, XU C, et al. The optimum extracting technology and antioxidant activity of polysaccharides from fruiting body of Phellinus igniarius[J]. The Food Industry, 2021, 42(11): 103-108. (in Chinese with English abstract) | |
| [28] | 李瑞雪, 王钰婷, 夏家凤, 等. 桑黄菌丝体中多糖提取工艺优化及其体外抗氧化活性分析[J]. 中国农学通报, 2019, 35(29): 143-150. |
| LI R X, WANG Y T, XIA J F, et al. Optimization of extraction process of polysaccharide of Phellinus igniarius mycelium and analysis of its antioxidant activity in vitro[J]. Chinese Agricultural Science Bulletin, 2019, 35(29): 143-150. (in Chinese with English abstract) | |
| [29] | 应瑞峰, 黄梅桂, 王耀松, 等. 桑黄子实体与桑黄菌丝多糖抗氧化活性研究[J]. 食品研究与开发, 2017, 38(21): 1-5. |
| YING R F, HUANG M G, WANG Y S, et al. Antioxidant activity of polysaccharides from Phellinus igniarius fruiting body and mycelium[J]. Food Research and Development, 2017, 38(21): 1-5. (in Chinese with English abstract) | |
| [30] | 李雨鸿, 殷朝敏, 范秀芝, 等. 桑黄提取物的体外抗氧化、降血糖及降尿酸活性[J]. 现代食品科技, 2022, 38(5): 71-80. |
| LI Y H, YIN C M, FAN X Z, et al. In vitro anti-oxidant, hypoglycemic, and hypouricemic activities of Sanghuangporus vaninii extracts[J]. Modern Food Science and Technology, 2022, 38(5): 71-80. (in Chinese with English abstract) | |
| [31] | 王伟科, 陆娜, 闫静, 等. 生长年限对段木栽培鲍姆桑黄子实体营养、活性成分及抗氧化活性的影响[J]. 菌物学报, 2021, 40(3): 668-680. |
| WANG W K, LU N, YAN J, et al. Effects of growth age on nutrition, content of active components and antioxidant activities of fruiting bodies of Sanghuangporus baumii cultivated on oak segment[J]. Mycosystema, 2021, 40(3): 668-680. (in Chinese with English abstract) | |
| [32] | LIU M M, ZENG P, LI X T, et al. Antitumor and immunomodulation activities of polysaccharide from Phellinus baumii[J]. International Journal of Biological Macromolecules, 2016, 91: 1199-1205. |
| [33] | YAYEH T, OH W J, PARK S C, et al. Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells[J]. Journal of Natural Medicines, 2012, 66(1): 49-54. |
| [34] | 吴声华, 戴玉成. 药用真菌桑黄的种类解析[J]. 菌物学报, 2020, 39(5): 781-794. |
| WU S H, DAI Y C. Species clarification of the medicinal fungus Sanghuang[J]. Mycosystema, 2020, 39(5): 781-794. (in Chinese with English abstract) | |
| [35] | 宋吉玲, 王伟科, 程俊文, 等. 野生桑黄的分离鉴定及培养特性研究[J]. 西北农林科技大学学报(自然科学版), 2020, 48(3): 129-136. |
| SONG J L, WANG W K, CHENG J W, et al. Isolation and cultivation of wild Sanghuangporue[J]. Journal of Northwest A & F University(Natural Science Edition), 2020, 48(3): 129-136. (in Chinese with English abstract) | |
| [36] | VALKO M, LEIBFRITZ D, MONCOL J, et al. Free radicals and antioxidants in normal physiological functions and human disease[J]. The International Journal of Biochemistry & Cell Biology, 2007, 39(1): 44-84. |
| [37] | 胡晓彤, 叶玉洁, 石光, 等. 桑黄子实体多糖的提取及其对D-半乳糖诱导的3T3细胞损伤的保护作用[J]. 食品科学, 2020, 41(19): 204-211. |
| HU X T, YE Y J, SHI G, et al. Extraction of polysaccharides from fruiting bodies of Phellinus igniarius and its protective effect on D-galactose induced 3T3 cell injury[J]. Food Science, 2020, 41(19): 204-211. (in Chinese with English abstract) |
| [1] | 刘晨星, 曹艳, 夏其乐. 多花黄精根须皂苷的提取工艺及其抗氧化活性研究[J]. 浙江农业学报, 2024, 36(5): 1144-1152. |
| [2] | 陆春霞, 刘开莉, 梁贵秋, 肖潇, 韦伟, 徐雯雯, 陈菁, 莫炳巧, 李小群, 黎尔纳, 黄旭华, 李安华, 韦师妮. 十一个桑黄菌株的形态学观察与分子鉴定[J]. 浙江农业学报, 2024, 36(4): 800-810. |
| [3] | 阮馨颖, 宋婷婷, 张作法, 金群力, 陈春, 蔡为明. 桑黄类大型真菌中多酚类物质研究进展[J]. 浙江农业学报, 2024, 36(3): 704-718. |
| [4] | 张璐, 李翘楚, 王增利, 丁强, 王鸿磊. 金耳类酵母型菌株分离与高产胞外多糖培养基优化[J]. 浙江农业学报, 2023, 35(5): 1154-1160. |
| [5] | 钟石, 曹英龙, 王文琼, 霍进喜, 孙雨晴, 宣利江, 李有贵. 人工栽培桑黄HPLC指纹图谱及主成分含量测定[J]. 浙江农业学报, 2023, 35(3): 658-665. |
| [6] | 马波, 陶震, 周瑞, 王雪, 吕茜茜, 孙士红, 王寒, 高金秋, 张楚涵, 陈凤清. 花叶万年青功能成分提取条件优化与活性探究[J]. 浙江农业学报, 2023, 35(2): 383-393. |
| [7] | 孙凤婷, 许振岚, 朱作艺, 张春荣, 汤涛, 赵学平, 盛清, 王强. 铁皮石斛的黄酮类成分测定及其生物可及性研究[J]. 浙江农业学报, 2023, 35(11): 2710-2719. |
| [8] | 邵宇辰, 穆宏磊, 陈杭君, 殷军艺, 房祥军, 吴伟杰, 刘瑞玲, 韩延超, 郜海燕. 肠道条件和粒径对加工山核桃体外消化的影响[J]. 浙江农业学报, 2022, 34(8): 1734-1742. |
| [9] | 刘晶, 胡晓, 杨贤庆, 陈胜军, 吴燕燕, 李来好, 戚勃, 邓建朝. 龙须菜蛋白质的提取及其酶解产物的抗氧化特性[J]. 浙江农业学报, 2022, 34(5): 1061-1072. |
| [10] | 郑美瑜, 王璐, 刘哲, 张文娟, 高浦, 陆胜民. 桑黄中抑制α-葡萄糖苷酶活性成分提取及其化学成分鉴定[J]. 浙江农业学报, 2022, 34(5): 949-958. |
| [11] | 李雪, 张玉, 王君虹, 朱作艺, 孙素玲, 刘志, 朱申龙, 沈国新, Agusti ROMERO, 王伟. 初榨橄榄油中多酚化合物的UPLC-FLD检测及其抗氧化活性研究[J]. 浙江农业学报, 2021, 33(5): 907-915. |
| [12] | 程安东, 汪本勤. 金寨县桑黄的菌种鉴定与生长特性研究[J]. 浙江农业学报, 2021, 33(12): 2234-2244. |
| [13] | 李园成, 孙宏, 吴逸飞, 姚晓红, 沈琦, 汤江武. 固态发酵棉籽仁的营养成分及其抗氧化活性[J]. 浙江农业学报, 2020, 32(4): 610-615. |
| [14] | 王明哲, 杨颖, 唐伟敏, 刘哲, 孙培龙, 陆胜民. 戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化[J]. 浙江农业学报, 2020, 32(2): 327-336. |
| [15] | 杨志, 李文义, 高云涛, 熊华斌, 陈毅坚, 杨慧娟. 响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究[J]. 浙江农业学报, 2020, 32(10): 1866-1872. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||
