浙江农业学报

• 动物科学 • 上一篇    下一篇

太湖鹅MyoG基因多态性与体重的相关性分析

  

  1. 1江苏农牧科技职业学院,江苏 泰州 225300;2 国家级水禽基因库,江苏 泰州 225300;3 江苏省畜牧总站,江苏 南京 210036)
  • 出版日期:2015-01-25 发布日期:2015-01-17

Association analysis between polymorphism of MyoG gene and body weight in Taihu goose

  1. (1 Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300,China; 2 National Gene Bank of Waterfowl Resources, Taizhou 225300,China; 3 The Animal Husbandry Bureau of Jiangsu Province, Nanjing 210036,China)
  • Online:2015-01-25 Published:2015-01-17

摘要: 以太湖鹅为材料,利用PCR\|SSCP技术检测该群体MyoG基因单核苷酸多态性(SNPs),分析SNPs与体重的关联性。结果表明,在MyoG基因第1外含子的108位碱基发生了C-T突变,形成了HH,HI,II 3种基因型。H等位基因频率为03271,I等位基因频率为06729。太湖鹅群体纯合度为05598,多态信息含量为03422。太湖鹅初生重在不同基因型间无显著性差异。在早期生长过程中,太湖鹅II型体重在不同周龄时处于最大值,杂合子HI型处于中间,HH型最小。在10周龄时,公鹅II型、 HI型的体重差异不明显,但均显著高于HH型体重;母鹅II型体重显著性高于HH型,HI型体重与HH型、 II型体重并无显著差异。这些关联分析表明I基因可能是太湖鹅早期增重的有利基因。

关键词: 肌细胞生成素, 太湖鹅, 单核苷酸多态性, 体重

Abstract: In this article, the SNPs of MyoG gene were detected in Taihu goose by the technology of PCR-SSCP. The relation between SNPs and body weight was analyzed. The results showed that one SNP(C-T) was found at the 108 bp in exon 1 of MyoG gene, which formed HH, HI and II genotypes. The frequency of allele H and I were 03271 and 06729, respectively. The homozygosity and PIC were 05598 and 03422. The birth weight of Taihu goose was not significantly different among different genotypes. The body weight of II genotype was highest at different weeks in the process of Taihu goose early growth, that of HI genotype was middle, and that of HH genotype was the lowest. When Taihu goose was 10-week-old, the body weight of II and HI male goose was not significantly different, but they were significantly higher than that of HH genotype in male goose. The body weight of II genotype female goose was significantly higher than that of HH genotype, but HI female goose was not significantly different from HH and II. The correlation analysis implied that allele I maybe were the favorable genes for body weight about Taihu goose.

Key words: MyoG, Taihu goose, SNP, body weight