浙江农业学报

• 植物保护 • 上一篇    下一篇

一株蓝莓菌根真菌的鉴定及对蓝莓生长的影响

  

  1. (1辽宁省农业科学院 微生物工程中心,辽宁 沈阳 110161;2中共蓬莱市委新农村建设办公室,山东 蓬莱 265600)
  • 出版日期:2015-03-25 发布日期:2015-03-31

Identification of blueberry mycorrhizal fungi and its effect on blueberry growth

  1. (1 Center of Microbiology Engineering, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;2 New Rural Construction Office of Penglai Municipal Party Committee, Penglai 265600, China)
  • Online:2015-03-25 Published:2015-03-31

摘要: 文章明确了一株能够明显促进蓝莓生长的菌根真菌的分类地位,通过形态观察结合18S rRNA测序比对结果表明该菌株为晶粒鬼伞菌种(Coprinus micaceus)。同时在蓝莓不同生长期接种该菌,系统研究该菌对蓝莓各生长时期生理生长方面的影响,结果表明接种该菌后,蓝莓组培苗、驯化苗株高分别比对照高出300%,561%,盆栽苗生长量比对照高出3454%。蓝莓顶端叶片激素含量是对照的23倍,蓝莓N,P吸收率显著提高,分别比对照增加128%,153%。接种菌根菌晶粒鬼伞菌对蓝莓的生长起到明显的促进作用。因此该菌株具有较好的应用前景,可以作为蓝莓生产储备菌株。

关键词: 蓝莓, 菌根真菌, 组培苗, 盆栽

Abstract: The taxonomic status of a mycorrhizal fungi strain, which could significantly promote blueberry growth, was determined by combined morphological and molecular methods. The inoculation effect of the strain on blueberry growth was studied systematically in physiological and biochemical aspects at different growth stage. Morphological observation of 18S combined with rDNA sequencing results showed that the species is Coprinus micaceus. After inoculating the bacteria, the plant height of blueberry plantlets and acclimated seedlings were 300%, 561% higher than the control, and ratio of diameter to height of the potted seedlings was 3454% higher than the control. The hormone content of blueberry top leaves was 23 times higher than the control; and N, P absorption rate of the blueberry increased significantly by 128% and 153%, respectively. The inoculation of Coprinus micaceus strain can significantly promote the blueberry growth. In conclusion, the strain has a good application prospect, and could be preserved for agricultural production.

Key words: blueberry, mycorrhizal fungi, tissue culture seedling, potted cultivation, growth promoting effect