浙江农业学报

• 动物科学 • 上一篇    下一篇

重金属对蚯蚓体内金属硫蛋白和谷胱甘肽过氧化物酶的影响

  

  1. (湖南农业大学 生物科学技术学院,湖南 长沙 410128)
  • 出版日期:2015-04-25 发布日期:2015-04-30

Effects of heavy metals on metallothionein and glutathione peroxidase in earthworms

  1. (College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)
  • Online:2015-04-25 Published:2015-04-30

摘要: 试验旨在探究土壤中不同含量Cu2+,Ag+,Cr6+对蚯蚓体内金属硫蛋白(MT)和谷胱甘肽过氧化物酶(GSH\|Px)活力的影响。将蚯蚓分别培养在含Cu2+,Ag+,Cr6+的土壤中,每种金属离子设0,5,50,100 mg·kg-1 4个含量,培养7 d后,测定蚯蚓体内MT含量和GSH\|Px活力。结果表明,5,50 mg·kg-1 Cr6+组MT值均显著高于对照组,100 mg·kg-1 Cr6+组显著低于对照组;50,100 mg·kg-1 Cu2+组MT值显著低于对照组。土壤中分别添加5,50,100 mg·kg-1 Cr6+和Ag+,蚯蚓体内GSH\|Px值均显著高于对照组;土壤中Cu2+含量为5,50 mg·kg-1时,GSH\|Px均显著高于对照组。土壤中Cr6+对蚯蚓体内MT和GSH\|Px的影响大于Cu2+和Ag+。

关键词: 蚯蚓, 铜离子, 银离子, 铬离子, 金属硫蛋白, 谷胱甘肽过氧化物酶

Abstract: Experiment was conducted to evaluate the effect of different concentrations of Cu2+, Ag+, Cr6+ in soil on earthworms. Earthworms were cultivated in different soil samples containing 0, 5, 50, 100 mg·kg-1 Cu2+, Ag+, Cr6+, respectively. After 7 d, metallothionein (MT) content and GSH\|Px enzyme activity were determined. It was shown that MT content in earthworm was higher under 5 or 50 mg·kg-1 Cr6+ treatment compared to the control, while it was lower under 100 mg·kg-1 Cr6+ treatment compared to the control. Under 50 or 100 mg·kg-1 Cu2+ treatment, MT content in earthworms was significantly lower than that of control. Under 5, 50 and 100 mg·kg-1 Cr6+ or Ag+ treatment, the enzyme activity of GSH\|Px was significantly higher than control. For Cu2+, the enzyme activity of GSH\|Px was significantly higher than the control under 5 or 50 mg·kg-1 treatment. Among these heavy metals, Cr6+ exhibited greater influence on MT content and GSH\|Px activity in earthworms than that of Cu2+ and Ag+.

Key words: earthworms, Cu2+, Ag+, Cr6+, metallothionein, glutathione peroxidase