浙江农业学报

• 环境科学 • 上一篇    下一篇

低电压下电生物膜脱氮体系中细菌多样性研究

  

  1. (1上海交通大学 环境科学与工程学院,上海 200240;2上海交通大学 材料科学与工程学院,上海 200240)
  • 出版日期:2015-05-25 发布日期:2015-05-19

Bacterial diversity of bio\|electrode denitrification process under a low voltage

  1. (1 School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; 2 School of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China)
  • Online:2015-05-25 Published:2015-05-19

摘要: 采用多阴极电生物膜反应器,在外加025 V驯化电压下得到了最佳脱氮效果,脱氮速率达1658 mg NO-3\|N·L-1·h-1,脱氮效率为不加电时的1859%。通过16S rDNA高通量测序的方法对多阴极电化学生物脱氮体系中细菌的多样性进行了研究。结果表明,反硝化体系中,Proteobacteria,Bacteroidetes和Firmicutes为优势菌种,在外加0250 V电压驯化后,微生物种群得到了筛选和富集,其中,Proteobacteria菌种在加电驯化后被选择性地富集。Proteobacteria菌中的Phyllobacterium和Caenispirillum属对于低电压强化电化学生物脱氮系统有着重要作用。

关键词: 电生物膜脱氮体系, 16S rDNA, 反硝化, 细菌多样性

Abstract: A multi\|cathode bio\|electrode film reactor was used for nitrate removal from wastewater. The nitrate removal rate reached 1658 mg NO-3\|N·L-1·h-1 at the applied voltage of 0250 V. 16S rDNA was used to investigate the bacterial communities on the cathodes. The results indicated that the dominant bacteria were Proteobacteria, Bacteroidetes and Firmicutes. After applying a voltage of 0250 V, the bacteria communities were selected and enriched, especially Proteobacteria. Genus of Phyllobacterium and Caenispirillum from Proteobacteria may have great contribution to the enhancement of nitrate removal rate in the bio\|electron denitrification process under a low voltage.

Key words: bio\, electrode denitrification process, 16S rDNA, denitrification, bacterial diversity