猪源莫拉菌的分离鉴定与部分生物学特性分析

doi:10.3969/j.issn.1004-1524.2018.01.05

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (1): 36-42.DOI: 10.3969/j.issn.1004-1524.2018.01.05

猪源莫拉菌的分离鉴定与部分生物学特性分析

廖倡宇^{1, 2}, 张鹏飞^{1, 2}, 王印^{1, 2, *}, 杨泽晓¹, 姚学萍¹, 姜睿姣¹, 邬旭龙^{1, 2}, 张博^{1, 2}, 周丽军^{1, 2}, 宋勇³

1.四川农业大学动物医学院,四川成都 611130;
2.动物疫病与人类健康四川省重点实验室,四川成都 611130;
3.天津瑞普生物技术股份有限公司,天津 300300

收稿日期:2017-05-28 出版日期:2018-01-20 发布日期:2018-02-09
通讯作者: 王印,E-mail: yaanwangyin@tom.com
作者简介:廖倡宇(1991—),男,四川绵竹人,硕士研究生,主要从事动物传染病病原微生物研究。E-mail: 709817049@qq.com
基金资助:
“十二五”国家科技支撑计划 (2013BAD12B04)

Identification and biological characteristics analysis of Moraxella from swine

LIAO Changyu¹, ZHANG Pengfei^{1, 2}, WANG Yin^{1, 2, *}, YANG Zexiao¹, YAO Xueping¹, JIANG Ruijiao¹, WU Xulong^{1, 2}, ZHANG Bo^{1, 2}, ZHOU Lijun^{1, 2}, SONG Yong³

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China;
3. Ruipu Biological Co., Ltd, Tianjin 300300, China

Received:2017-05-28 Online:2018-01-20 Published:2018-02-09

摘要/Abstract

摘要： 从病死仔猪心脏中分离到一株革兰阴性菌,对该细菌进行形态学观察、生化鉴定、16S rDNA序列测定,确认该菌为猪源莫拉菌属亚种,将该菌株命名为猪源莫拉菌-ZY20001。对菌株进行致病性试验、药敏试验和部分耐药基因的检测。结果显示,该菌引起小鼠胸腔积液,不致死,对红霉素、青霉素、多粘菌素等21种抗生素药物表现不同程度的敏感,对哌拉西林耐药。菌株ZY20001具有TEM型β-内酰胺酶耐药基因,表型与基因型不符。该实验可为深入研究莫拉菌的分类进化及药物治疗提供参考依据,同时对青霉素耐药表型与基因型关系所做的初步探讨也丰富了流行病学调查资料。

关键词: 莫拉菌属, 分离鉴定, 16S rDNA, 耐药性

Abstract: A gram-negative bacterium was isolated from the heart of dead piglets, and morphological observation, biochemical identification and 16S rDNA sequence were performed. The strain was identified as Moraxella porci sp., which was named as Moraxella porci-ZY20001. The pathogenicity test, drug susceptibility test and partial resistance gene were detected in strain ZY20001. The results showed that the bacteria caused the pleural effusion in mice and showed varying degrees of sensitivity to erythromycin, penicillin, polymyxin and other antibiotics (a total of 21 kinds), except piperacillin. The strain ZY20001 had a TEM-type β-lactamase-resistant gene, and the phenotype was not consistent with the genotype. This study provided scientific reference for the further study of the evolution and drug therapy of Moraxella, and the analysis of relationship between penicillin resistance phenotype and genotype would enrich the epidemiological survey data.

Key words: Moraxella, isolation and identification, 16S rDNA, drug resistance

中图分类号:

廖倡宇, 张鹏飞, 王印, 杨泽晓, 姚学萍, 姜睿姣, 邬旭龙, 张博, 周丽军, 宋勇. 猪源莫拉菌的分离鉴定与部分生物学特性分析[J]. 浙江农业学报, 2018, 30(1): 36-42.

LIAO Changyu, ZHANG Pengfei, WANG Yin, YANG Zexiao, YAO Xueping, JIANG Ruijiao, WU Xulong, ZHANG Bo, ZHOU Lijun, SONG Yong. Identification and biological characteristics analysis of Moraxella from swine[J]. , 2018, 30(1): 36-42.

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猪源莫拉菌的分离鉴定与部分生物学特性分析

Identification and biological characteristics analysis of Moraxella from swine

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