2019年浙江和福建沙门氏菌和弯曲杆菌的耐药性分析

doi:10.3969/j.issn.1004-1524.2020.12.13

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (12): 2218-2225.DOI: 10.3969/j.issn.1004-1524.2020.12.13

2019年浙江和福建沙门氏菌和弯曲杆菌的耐药性分析

唐标¹^,²(), 陈凌云¹^,², 罗怡¹^,²^,³, 施杏芬⁴, 胡骥², 陈怡飞¹^,², 钱鸣蓉¹^,², 杨华¹^,²^,^*()

1.浙江省农业科学院农产品质量安全危害因子与风险防控国家重点实验室(筹),浙江杭州 310021
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021
3.中国计量大学生命科学学院,浙江杭州 310018
4.浙江省动物疫病预防控制中心,浙江杭州 310020

收稿日期:2020-06-04 出版日期:2020-12-25 发布日期:2020-12-25
通讯作者: 杨华
作者简介:*杨华,E-mail: yanghua@zaas.ac.cn
唐标(1987—),男,江苏徐州人,博士,助理研究员,从事动物源细菌耐药性研究。E-mail: tb_411@163.com
基金资助:
农产品质量安全标准化生产示范创建(“一县一品一策”)重大专项(ZJNY2020001);国家自然科学基金青年科学基金(31700007);浙江省重点研发计划(2020C02031);省部共建农产品质量安全危害因子与风险防控国家重点实验室项目(2010DS700124-ZZ2008)

Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019

TANG Biao¹^,²(), CHEN Lingyun¹^,², LUO Yi¹^,²^,³, SHI Xingfen⁴, HU Ji², CHEN Yifei¹^,², QIAN Mingrong¹^,², YANG Hua¹^,²^,^*()

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products (in Prepared), Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
4. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310020, China

Received:2020-06-04 Online:2020-12-25 Published:2020-12-25
Contact: YANG Hua

摘要/Abstract

摘要：

为了解2019年浙江和福建动物源沙门氏菌和弯曲杆菌流行情况及其耐药特征,于2019年在浙江和福建共10市随机采集新鲜粪便和肛拭子样品共计1 480份,参考国家标准方法分离沙门氏菌和弯曲杆菌,利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)方法进行菌种鉴定,并使用微量肉汤稀释法分别检测沙门氏菌和弯曲杆菌对16种和9种抗生素的最小抑菌浓度(MIC)。本研究从2省样品中共计分离了100株沙门氏菌和100株弯曲杆菌,其中94株沙门氏菌被鉴定出血清型,包括11种血清型,优势血清型为策维埃(20.2%)、肯塔基(15.9%)、韦太夫雷登(15.9%)、鼠伤寒(14.9%)、肠炎(14.9%)。药敏检测结果显示:分离出的沙门氏菌对四环素的耐药率最高(76%),其次为氨苄西林(70%)、磺胺异恶唑(65%),对奥格门丁(6%)的耐药率相对较低,对美罗培南全部敏感;分离出的弯曲杆菌对环丙沙星(99%)、萘啶酸(88%)和四环素(87%)的耐药率最高,对庆大霉素的耐药率较低(23%)。结果表明,浙江和福建2省动物源沙门氏菌和弯曲杆菌耐药水平较高,多重耐药情况普遍,影响畜产品质量安全,可对公共卫生安全构成威胁,需要提高警惕。

关键词: 沙门氏菌, 弯曲杆菌, 耐药性

Abstract:

To understand the epidemicity and antimicrobial resistance of Salmonella and Campylobacter in Zhejiang and Fujian in 2019, 1 480 fecal and anal swab samples were randomly collected in 10 cities of Zhejiang Province and Fujian Province in 2019. Salmonella and Campylobacter strains were isolated by the method of national standards of China, and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was used for identification. The minimal inhibitory concentration (MIC) of Salmonella and Campylobacter strains against 16 and 9 antibiotics, respectively, was determined by microdilution broth method. A total of 100 Salmonella strains and 100 Campylobacter strains were isolated in the present study. Among them, 11 serotypes were identified in 94 Salmonella strains. The dominant serotypes were Tsevie (20.2%), Kentucky (15.9%), Weltevreden (15.9%), Typhimurium (14.9%) and Enteritidis (14.9%). The results of drug sensitivity test showed that the resistance rates of the isolated Salmonella strains to tetracycline was the highest (76%), which was followed by ampicillin (70%) and sulfamethoxazole (65%). While, the drug resistance rate of Salmonella strains to amoxicillin/clavulanate was low (6%), and all the isolated Salmonella strains were sensitive to meropenem. In addition, the isolated Campylobacter strains had the highest resistance rate to ciprofloxacin (99%), nalidixic acid (88%) and tetracycline (87%), while the resistance rate to gentamicin was the lowest (23%). All in all, the antimicrobial resistance level of pathogenic bacteria from animals was high in Zhejiang Province and Fujian Province, which would affect the quality and safety of animal products and threaten the public health safety.

Key words: Salmonella, Campylobacter, antimicrobial resistance

中图分类号:

S852.6

唐标, 陈凌云, 罗怡, 施杏芬, 胡骥, 陈怡飞, 钱鸣蓉, 杨华. 2019年浙江和福建沙门氏菌和弯曲杆菌的耐药性分析[J]. 浙江农业学报, 2020, 32(12): 2218-2225.

TANG Biao, CHEN Lingyun, LUO Yi, SHI Xingfen, HU Ji, CHEN Yifei, QIAN Mingrong, YANG Hua. Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2218-2225.

图/表 6

参考文献 25

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序号 ID	耐药谱 Drug-resistant pattern	个数 Number	百分比 Percentage/%
1	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-CAZ-ENR-OFL	18	18.0
2	—	15	15.0
3	AMP-SPT-TET-FFC-SF-SXT	8	8.0
4	AMP-TET-SF	6	6.0
5	TET-FFC-SF-SXT	5	5.0
6	AMP-GEN-SPT-TET-FFC-SF-SXT	5	5.0
7	TET-FFC	5	5.0
8	AMP-GEN-TET	4	4.0
9	AMP-GEN-TET-FFC-SF-SXT	4	4.0
10	AMP-A/C-GEN-SPT-TET-FFC-SF-SXT	4	4.0
11	AMP-GEN-TET-CL	3	3.0
12	AMP-SPT-FFC-SXT-CEF-ENR	2	2.0
13	AMP-SF-CL	2	2.0
14	AMP-TET-SF-CL	2	2.0
15	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-ENR-OFL	2	2.0
16	AMP-TET-FFC-SF-SXT	2	2.0
17	TET	1	1.0
18	AMP-A/C-SPT-TET-SXT-CL	1	1.0
19	SPT	1	1.0
20	AMP-GEN-TET-FFC	1	1.0
21	AMP-GEN-CL	1	1.0
22	AMP-TET	1	1.0
23	TET-SF-CL	1	1.0
24	AMP-SPT-SF-SXT-CL	1	1.0
25	AMP-A/C-SPT-TET-SF-CEF-CAZ-CL	1	1.0
26	AMP-TET-FFC-SF-ENR	1	1.0
27	SF	1	1.0
28	AMP-SPT-FFC-SF-SXT	1	1.0
29	TET-SF-ENR	1	1.0

序号 ID	耐药谱 Drug-resistant pattern	个数 Number	百分比 Percentage/%
1	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-CAZ-ENR-OFL	18	18.0
2	—	15	15.0
3	AMP-SPT-TET-FFC-SF-SXT	8	8.0
4	AMP-TET-SF	6	6.0
5	TET-FFC-SF-SXT	5	5.0
6	AMP-GEN-SPT-TET-FFC-SF-SXT	5	5.0
7	TET-FFC	5	5.0
8	AMP-GEN-TET	4	4.0
9	AMP-GEN-TET-FFC-SF-SXT	4	4.0
10	AMP-A/C-GEN-SPT-TET-FFC-SF-SXT	4	4.0
11	AMP-GEN-TET-CL	3	3.0
12	AMP-SPT-FFC-SXT-CEF-ENR	2	2.0
13	AMP-SF-CL	2	2.0
14	AMP-TET-SF-CL	2	2.0
15	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-ENR-OFL	2	2.0
16	AMP-TET-FFC-SF-SXT	2	2.0
17	TET	1	1.0
18	AMP-A/C-SPT-TET-SXT-CL	1	1.0
19	SPT	1	1.0
20	AMP-GEN-TET-FFC	1	1.0
21	AMP-GEN-CL	1	1.0
22	AMP-TET	1	1.0
23	TET-SF-CL	1	1.0
24	AMP-SPT-SF-SXT-CL	1	1.0
25	AMP-A/C-SPT-TET-SF-CEF-CAZ-CL	1	1.0
26	AMP-TET-FFC-SF-ENR	1	1.0
27	SF	1	1.0
28	AMP-SPT-FFC-SF-SXT	1	1.0
29	TET-SF-ENR	1	1.0

序号 ID	耐药谱 Drug-resistant pattern			个数 Number	百分比 Percentage/%
1	CIP-NAL-TET			21	21.0
2	CIP-NAL-TET-CLI-ERY-AZM-TEL			11	11.0
3	CIP-TET-CLI-ERY-AZM-FFC			11	11.0
4	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL			8	8.0
5	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL-FFC			7	7.0
6	CIP-NAL-TET-CLI			5	5.0
7	CIP-NAL			5	5.0
8	CIP-NAL-TET-AZM			3	3.0
9	CIP-NAL-TET-FFC			3	3.0
10	CIP-NAL-CLI-AZM			3	3.0
11	CIP-NAL-TET-CLI-ERY-AZM-TEL-FFC			3	3.0
12	CIP-NAL-TET-CLI-AZM			2	2.0
13	CIP-NAL-TET-CLI-AZM-TEL			2	2.0
14	CIP-NAL-TET-CLI-AZM-TEL-FFC			2	2.0
15	CIP-NAL-GEN-CLI-ERY-AZM-TEL			2	2.0
16	CIP-NAL-GEN-TET-CLI-AZM-TEL-FFC			2	2.0
17		CIP-NAL-TET-TEL-FFC	1		1.0
18		CIP-NAL-TET-AZM-TEL	1		1.0
19		—	1		1.0
20		CIP-NAL-GEN-TET	1		1.0
21		CIP-NAL-GEN-TET-CLI-TEL	1		1.0
22		CIP-NAL-TET-CLI-TEL	1		1.0
23		CIP-NAL-GEN-TET-CLI	1		1.0
24		CIP-NAL-GEN-TET-CLI-ERY-AZM	1		1.0
25		CIP-NAL-TEL	1		1.0
26		CIP-NAL-CLI	1		1.0

序号 ID	耐药谱 Drug-resistant pattern			个数 Number	百分比 Percentage/%
1	CIP-NAL-TET			21	21.0
2	CIP-NAL-TET-CLI-ERY-AZM-TEL			11	11.0
3	CIP-TET-CLI-ERY-AZM-FFC			11	11.0
4	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL			8	8.0
5	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL-FFC			7	7.0
6	CIP-NAL-TET-CLI			5	5.0
7	CIP-NAL			5	5.0
8	CIP-NAL-TET-AZM			3	3.0
9	CIP-NAL-TET-FFC			3	3.0
10	CIP-NAL-CLI-AZM			3	3.0
11	CIP-NAL-TET-CLI-ERY-AZM-TEL-FFC			3	3.0
12	CIP-NAL-TET-CLI-AZM			2	2.0
13	CIP-NAL-TET-CLI-AZM-TEL			2	2.0
14	CIP-NAL-TET-CLI-AZM-TEL-FFC			2	2.0
15	CIP-NAL-GEN-CLI-ERY-AZM-TEL			2	2.0
16	CIP-NAL-GEN-TET-CLI-AZM-TEL-FFC			2	2.0
17		CIP-NAL-TET-TEL-FFC	1		1.0
18		CIP-NAL-TET-AZM-TEL	1		1.0
19		—	1		1.0
20		CIP-NAL-GEN-TET	1		1.0
21		CIP-NAL-GEN-TET-CLI-TEL	1		1.0
22		CIP-NAL-TET-CLI-TEL	1		1.0
23		CIP-NAL-GEN-TET-CLI	1		1.0
24		CIP-NAL-GEN-TET-CLI-ERY-AZM	1		1.0
25		CIP-NAL-TEL	1		1.0
26		CIP-NAL-CLI	1		1.0

2019年浙江和福建沙门氏菌和弯曲杆菌的耐药性分析

Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019

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