米槁根际细菌对果实药用活性成分的影响及其PICRUST功能预测分析

doi:10.3969/j.issn.1004-1524.2022.09.03

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (9): 1837-1848.DOI: 10.3969/j.issn.1004-1524.2022.09.03

米槁根际细菌对果实药用活性成分的影响及其PICRUST功能预测分析

田秀¹(), 童炳丽², 谢元贵¹^,³^,^*(), 廖小锋⁴, 吴婷婷¹, 刘济明¹

1.贵州大学林学院,贵州,贵阳 550025
2.贵州师范大学生命科学学院,贵州贵阳550025
3.贵州科学院,贵州贵阳 550001
4.贵州省山地资源研究所,贵州贵阳550001

收稿日期:2021-12-17 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 谢元贵
作者简介:*谢元贵,E-mail: yuanguixie@163.com
田秀(1997—),女,苗族,贵州铜仁人,硕士研究生,研究方向为恢复生态学、植物生态学。E-mail: 626052286@qq.com
基金资助:
贵州省科技计划(黔科合成果〔2021〕一般130);贵州省科技计划(黔科合基础-ZK〔2021〕一般100);贵州省土地绿色整治工程研究中心(黔发改投资〔2019〕886号);贵州科学院资助项目(黔科院人才〔2019〕07号);贵州科学院资助项目(黔科院科专合字〔2019〕06号);贵阳国家高新区科技计划(GXYF-2017-003);贵阳国家高新区科技计划(GXYF-2018-003)

Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

TIAN Xiu¹(), TONG Bingli², XIE Yuangui¹^,³^,^*(), LIAO Xiaofeng⁴, WU Tingting¹, LIU Jiming¹

1. College of Forestry, Guizhou University, Guiyang 550025, China
2. College of Life Sciences, Guizhou Normal University, Guiyang 550025, China
3. Guizhou Academy of Sciences, Guiyang 550001, China
4. Guizhou Provincial Institute of Mountain Resources, Guiyang 550001, China

Received:2021-12-17 Online:2022-09-25 Published:2022-09-30
Contact: XIE Yuangui

摘要/Abstract

摘要：

研究道地药材米槁根际土壤细菌与果实活性成分含量间的关系,对米槁培育管理和果实活性成分增产有重要作用。采集黔、滇、桂3省交界处的米槁根际土壤与果实,利用高通量测序技术对米槁根际土壤细菌进行测序,同时测定土壤的理化特性和米槁果实的活性成分。结果表明,产自天峨的1,8-桉叶素、α-松油醇、柠檬烯含量最多,望谟的香桧烯含量最高;Shannon指数在望谟最高,为6.315 8。几个采样点的优势菌门一致,为变形菌门、酸杆菌门、放线菌门和绿弯菌门,但优势菌属不同;在属水平上,优势菌属主要受pH值、氮含量的影响,大多数菌属与活性成分呈正相关;PICRUST功能预测结果表明,活性成分主要受细菌能量与物质的转运与代谢功能的影响,说明土壤中pH值和氮可通过影响根际土壤细菌的多样性,从而影响果实中药用活性成分的积累。

关键词: 米槁, 根际土壤, 细菌多样性, 药用活性成分

Abstract:

Research on the relationship between soil rhizobacteria of authentic medicinal material Cinnamomum migao and the fruit medicinal active ingredient plays an important role on C. migao cultivation and management and yield improvement of active components. Rhizosphere soils and fruits of C. migao at the junction of Guizhou, Yunnan and Guangxi were collected. High-throughput sequencing technology was used to analyze the gene sequences of soil rhizobacteria of C. migao, soil physicochemical properties and fruit active components were determined as well. As a result, fruit active components 1,8-cineole, α-terpineol and limonene were highest in Tian’e, and cypressene was in Wangmo. Bacterial Shannon index in Wangmo was 6.315 8, higher than other sampling sites. The dominant bacterial phylum in all samples were Proteobacteria, Acidobacteria, Actinobacteria, and Chloroflexi, but it was varied in dominant genera. At the genus level, dominant bacterial genera were mainly affected by pH value and nitrogen content, most genera were positively related with active ingredients. The PICRUST functional prediction results showed that active components were mainly affected by transport and metabolism of bacterial energy and substances, indicating that pH value and nitrogen content in soil could influence the accumulation of fruit medicinal active components by changing rhizophere bacterial diversity.

Key words: Cinnamomum migao H.W.Li, rhizosphere soil, bacterial diversity, medicinal active ingredients

中图分类号:

S567.19

田秀, 童炳丽, 谢元贵, 廖小锋, 吴婷婷, 刘济明. 米槁根际细菌对果实药用活性成分的影响及其PICRUST功能预测分析[J]. 浙江农业学报, 2022, 34(9): 1837-1848.

TIAN Xiu, TONG Bingli, XIE Yuangui, LIAO Xiaofeng, WU Tingting, LIU Jiming. Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1837-1848.

图/表 11

表1 米槁根际土壤理化特性

Table 1 Physical and chemical properties of rhizosphere soil of C. migao

样本 Sample	pH	有机质 Organic matter/ (g·kg^-1)	全氮 Total nitrogen/ (g·kg^-1)	全钾 Total potassium/ (g·kg^-1)	全磷 Total phosphorus/ (g·kg^-1)	碱解氮 Available nitrogen/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)	有效钾 Available potassium/ (mg·kg^-1)
LD	4.84± 0.02 de	8.83± 0.26 a	2.29± 0.25 a	8.07± 0.15 f	0.265± 0.01 a	226.33± 7.10 c	816.07± 0.83 a	3.34± 0.12 c
WM	5.82± 0.19 c	7.51± 0.41 b	1.82± 0.11 a	32.70± 0.31b	0.175± 0.01 cde	166.83± 15.17d	819.70± 0.30 a	6.06± 0.14 a
CH	4.94± 0.02 d	6.34± 0.18 c	2.15± 0.05 a	31.27± 0.47bc	0.178± 0.01 cd	250.83± 7.65 bc	320.37± 3.43 c	3.31± 0.93 c
ZF	4.19± 0.01 f	5.92± 0.03 c	1.73± 0.19 a	23.90± 0.46d	0.150± 0.01 ef	245.00± 6.06 bc	259.67± 8.17 d	3.41± 0.03 c
TE	4.80± 0.01de	4.93± 0.63d	2.24± 0.28a	28.80± 1.36c	0.140± 0.01f	252.00± 4.04bc	803.37± 6.56a	4.31± 0.01 bc
LY	6.82± 0.01 a	8.18± 0.15 ab	2.47± 0.08 a	37.50± 0.29a	0.153± 0.01 def	268.33± 10.17b	818.80± 1.08 a	4.97± 0.11 ab
FN	5.72± 0.03 c	6.14± 0.25 c	2.61± 0.25 a	18.83± 0.23e	0.205± 0.01 b	246.17± 9.11 bc	817.60± 0.30 a	4.48± 0.10 bc
NP	4.56± 0.15 e	3.73± 0.18 e	2.24± 0.32 a	30.17± 1.34c	0.145± 0.01 f	166.83± 8.41 d	654.33± 11.62 b	5.49± 0.06 ab
LB	6.11± 0.01 b	8.19± 0.21 ab	2.73± 0.19 a	36.00± 0.30a	0.192± 0.01 bc	325.5± 2.02 a	816.70± 0.30 a	4.70± 0.04 bc

图1 米槁果实药用活性成分的含量 LD,罗甸;WM,望谟;CH,册亨;ZF,贞丰;TE,天峨;LY,乐业;FN,富宁;NP,那坡;LB,荔波。柱上无相同小写字母表示差异显著(P<0.05)。下同。

Fig.1 Content of medicinal active ingredients in C. migao fruits LD, Luodian; WM, Wangmo; CH, Ceheng; ZF, Zhenfeng; TE, Tian’e; LY, Leye; FN, Funing; NP, Napo; LB, Libo. Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

表2 米槁根际土壤的细菌Alpha多样性

Table 2 Bacterial Alpha diversity of rhizosphere soil in C. migao

样本 Sample	可操作序列数 OTUs	有效序列 Valid sequences	香农指数 Shannon index	辛普森指数 Simpson index	覆盖度 Coverage/%
LY	1 199	35 789	6.052 3	0.007 3	99.19
CH	1 430	42 882	6.080 1	0.006 2	99.50
WM	1 052	30 500	6.315 8	0.003 7	99.40
LB	1 028	43 272	5.323 5	0.017 9	99.31
LD	929	42 058	5.722 1	0.008 9	99.42
ZF	1 286	33 506	5.563 6	0.009 9	99.60
TE	1 495	33 834	5.871 5	0.008 6	99.33
NP	1 315	29 592	5.599 0	0.007 7	99.64
FN	1 382	27 740	5.873 5	0.008 6	99.25

图2 门水平上的物种相对丰度分布图

Fig.2 Distribution of relative abundance of species on phylum level

图3 纲水平上的物种相对丰度分布

Fig.3 Distribution of relative abundance of species on class level

图4 属水平上的物种分布热图

Fig.4 Species distribution heatmap on genus level

图5 根际土壤特性与属水平上细菌的RDA分析和heatmap图 TN为全氮,AN为碱解氮;TK为全钾,AK为有效钾;TP为全磷,AP为速效磷;OM为有机质。

Fig.5 RDA analysis and correlation heatmap between rhizosphere soil characteristics and bacteria on genus level TN means total nitrogen, AN means alkaline hydrolysis nitrogen; TK means total potassium, AK means available potassium; TP means total phosphorus, AP means available phosphorus; OM means organic matter.

图6 药用活性成分与属水平上细菌的RDA分析和热图

Fig.6 RDA analysis and correlation heatmap between medicinal active ingredients and bacteria on genus level

图7 基于KEGG pathway的功能基因相对丰度分布图

Fig.7 Relative abundance distribution of functional genes based on KEGG pathway

图8 基于GOC的功能基因相对丰度分布图 J,翻译、核糖体结构和生物发生;Z,细胞骨架;N,细胞运动;L,复制、修复和重组;F,核苷酸的运输和代谢;G,碳水化合物运输与代谢;K,转录;D,细胞周期控制、细胞分裂、染色体分配;E,氨基酸的转运和代谢;S,功能未知;C,能量生产和转化;B,染色质结构和动力学;A,RNA加工和修饰;M,细胞壁/膜/包膜生物发生;W,细胞外结构;Q,次生代谢物的生物合成、运输和分解代谢;U,细胞内运输、分泌和囊泡运输;V,防御机制;O,翻译后修饰、蛋白质周转、调控;H,辅酶运输和代谢;P,无机离子转运与代谢;Y,核结构;I,脂质运输与代谢;R,一般功能预测;T,信号传导机制。下同。

Fig.8 Relative abundance distribution of functional genes based on GOC J, Translation, ribosome structure and biogenesis; Z, Cytoskeleton; N, cell motility; L, Replication, repair and recombination; F, Nucleotide transport and metabolism; G, Carbohydrate transport and metabolism; K, Transcription ; D, Cell cycle control, cell division, chromosome partitioning; E, Amino acid transport and metabolism; S, Function unknown; C, Energy production and conversion; B, Chromatin structure and dynamics; A, RNA processing and modification; M, Cell wall/membrane/envelope biogenesis; W, Extracellular structures; Q, Secondary metabolites biosynthesis, transport, and catabolism; U, Intracellular transport, secretion, and vesicular trafficking; V, Defense mechanisms; O, Posttranslational modification, protein turnover, chaperones; H, Coenzyme transport and metabolism; P, Inorganic ion transport and metabolism; Y, Nuclear structure; I, Lipid transport and metabolism; R, General functional prediction only; T, Signal transduction mechanisms. The same as below.

图9 根际土壤细菌的功能与果实活性成分积累的RDA分析

Fig.9 RDA analysis of soil bacteria function in the rhizosphere and accumulation of active ingredients in the fruit

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米槁根际细菌对果实药用活性成分的影响及其PICRUST功能预测分析

Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

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