浙江农业学报

• 动物科学 • 上一篇    下一篇

藏猪与雅南猪IGF\|I基因的克隆与序列分析

  

  1. (1 四川农业大学 动物医学院动物微生态研究中心,四川 成都 611130;2 动物疫病与人类健康四川省重点实验室,四川成都611130)
  • 出版日期:2015-08-25 发布日期:2015-08-21

Cloning and sequence analysis of IGF\|I gene in Tibetan pig and Yanan pig#br#


  1. (1 Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China)
  • Online:2015-08-25 Published:2015-08-21

摘要: 为研究IGF\|I基因对藏猪、雅南猪生长发育的影响,以GenBank中猪IGF\|I基因序列(登录号:DQ1211321)设计引物,提取藏猪、雅南猪肝脏组织总RNA,应用RT\|PCR技术克隆出IGF\|I基因,分别将藏猪、雅南猪IGF\|I基因克隆到PMD19\|T载体上,构建重组质粒pMD19\|T\|IGF\|I, 经PCR鉴定、测序分析得该基因大小为612 bp,包含一个完整的ORF,该ORF共有462个碱基,编码153个氨基酸;所测得的藏猪和雅南猪基因序列与Muller等报道的猪IGF\|I基因编码序列高度同源,序列比对发现,藏猪的同源性为100%,雅南猪的为9961%;藏猪IGF\|I与雅南猪IGF\|I基因序列的同源性为9961%;雅南猪在258 bp处发生A→G突变,但其编码的氨基酸未发生改变。

关键词: 藏猪, 雅南猪, IGF\, I基因, 序列分析

Abstract: To study the impact of IGF\|I gene on the growth of Tibetan pig and Yanan pig, a pair of primers was designed by sus scrofa IGF\|I gene sequence from GenBank(Accession number:DQ1211321),the total RNAs were extracted by using Trizol from the livers of Tibetan pig and Yanan pig and used as template to amplify IGF\|I gene by RT\|PCR,the amplified fragments were cloned into pMD19\|T vector and the recombinant plasmids pMD19T\|IGF\|I were constructed and sequenced. The sequencing results indicated that the IGF\|I gene consisted of 612 nucleotides,containing a complete ORF of 462 bp ecoding 153 amino acids. The two IGF\|I gene sequences shared high homology with the porcine IGF\|I gene reported by Muller, et al.,which had an identity of 100% in Tibetan pig and 9961% in Yanan pig. It shared 9961% homology between the Tibetan pig and Yanan pig IGF\|I gene. An A→G mutation occurred in the 258 bp of Yanan pig, but the corresponding amino acid was not changed.

Key words: Tibetan pig, Yanan pig, IGF\, I gene, sequence analysis