浙江农业学报

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礁膜Monostroma nitidum的形态学观察和分子鉴定

  

  1. (1 上海海洋大学 水产与生命学院,上海 201306;2 广东海洋大学 水产学院,广东 湛江 524025;3 奉化益珍海藻科技有限公司,浙江 奉化 315500)
  • 出版日期:2015-09-25 发布日期:2015-09-23

Morphological and molecular study of Monostroma nitidum#br#

  1. (1 College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306, China; 2 College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 3 Fenghua Yizhen Alage Technology Co. Ltd, Fenhua 315500, China)
  • Online:2015-09-25 Published:2015-09-23

摘要: 采用形态学观察结合分子生物学的方法,对采自浙江和广东沿海的礁膜进行了初步的种类鉴定。形态学观察结果表明,藻体柔软粘滑,为片状、膜质、单层细胞;ITS序列分析结果显示,浙江的12个样品和广东的7个样品均聚于礁膜(Monostroma nitidum)分枝,将其确认为礁膜Monostroma nitidum。礁膜有无性生殖和有性生殖2种发育方式,是一种经济价值较高的海藻,具有较好的开发利用前景。

关键词: 礁膜, 形态学, ITS, 分子鉴定

Abstract: Monostroma collected from Zhejiang Province and Guangdong Province was studied in this paper.  Identification was conducted by combining morphological observation and molecular biological methods. At the morphological level, the thallis were soft and slimy, their cross sections were flake, membranous and had monolayer cells. At the molecular level, the result of ITS sequencing analysis showed that all collected samples belonged to the same phylogenetic group, and were identified as Monostroma nitidum. There were two growth ways in M.nitidum: one was asexual reproduction, the other was sexual reproduction. M.nitidum was of great economic value, and it had good prospects for the development and utilization.

Key words: Monostroma nitidum, morphology, ITS, molecular identification