关键词：mCherry红色荧光蛋白, 转基因, 过量表达, 水稻

Abstract： Overexpression is an important approach for functional characterization of plant genes and genetic improvement of crop plants. To improve the efficiency of characterization of transgenic plants， a transformation vector with the maize （Zea mays） ubiquitin\|1 promoter （P\|Ubi） and the pea （Pisum sativum） T3A\|polyA sequence in the overexpression cassette was constructed using the mCherry red fluorescent protein as a reporter. The coding sequences of four receptor\|like kinases of rice （Oryza sativa） were inserted into the overexpression cassette of the vector and then transformed into the rice cultivar Taijing\|394， respectively. mCherry fluorescence assay of the T1 transgenic seeds showed that on average， 625% of the T1 lines segregated with a 3∶1 ratio. Based on the results of real\|time quantitative reverse\|transcription polymerase chain reaction assays， the relative gene\|expression levels of the overexpressed genes in the transgene\|positive plants were significantly higher than those of the genes in the negative segregant and untransformed recipient cultivar. The vector system can be used for large\|scale screening of transgenic progeny and obtaining of transgenic lines with target genes overexpressed， so as to promote the functional characterization of plant genes and the application of transgenic technology.

Key words: mCherry red fluorescent protein, transgene, overexpression, rice （Oryza sativa）