关键词: 卵清蛋白基因, 调控序列, 载体, 荧光素酶活性

Abstract:  There was only one allele of ovalbumin gene in the chicken genome， but it could synthesize and secrete 2 g protein per day， which were accounting for more than 50% of the albumin protein and became the preferred choice in the regulation of exogenous gene expression. This study aimed to identify promoter enhancer and tissuespecific regional location factor by screening the optimization of ovalbumin gene promoter. The upstream -922～-2 073 and -2 801～-3 100 of ovalbumin promoter were divided into 12 regional which average sequence length were about 150 bp， and inserted into the upper reaches of -921～+38 sequences， those 12 series successfully constructed expression vectors provided materials for further optimization promoter with a shortened version. The first intron region of ovalbumin promoter was truncated around 300 bp of mini intron sequences， and successfully constructed 8 mini intron series of vectors. We also successfully separated chicken oviduct epithelial cells and optimized the electricity transfection conditions. In this study， the promoter region with strongest activity pGL4UP1412 and pGL4miniintron3 were screened through detected luciferase activity of the initial screening recombinant plasmid and intron recombinant， while inferred several regions containing enhancer sequence.

Key words: ovalbumin gene, regulation sequences, vector, luciferase activity