关键词: 鸭, 胚胎, 成肌细胞, GHR, IGF1

Abstract: To investigate the expression patterns of growth hormone receptor （GHR） and insulinlike growth factor1 （IGF1） in duck skeletal muscle myoblasts， fluorescent realtime quantitative PCR was used to detect the distribution profile of GHR and IGFI mRNA in skeletal muscle myoblasts of Jinding and Gaoyou ducks at the embryonic day 13， 15， 17， 19， 21 and 23. The results showed that the expressions of IGF1 of Gaoyou ducks in breast and leg muscle myoblasts were significantly higher than that of Jinding ducks at embryonic day 13 （P<0.05）; the embryonic day 17 was the common expression peak of GHR and IGF1 both in Gaoyou ducks and Jinding ducks; and then， significantly decreased. But at the embryonic day 19 and 21， the expressions of GHR and IGF1 in Gaoyou ducks breast muscle myoblasts were significantly higher than those of Jinding ducks （P<0.05）， while there was no significant difference between varieties in the leg muscle myoblasts （P>0.05）. The expression of GHR had a significantly linear positive correlation with the expression of IGF1 in breast and leg muscle myoblasts（P< 0.01）. The results showed that the expression pattern of GHR was consistent with the expression of IGF1 between breeds; the expressions of GHR and IGF1 mRNA in the skeletal muscle myoblasts of duck embryo showed tissue specificity， and there might be a positive regulation mechanism between them.

Key words: duck, embryo, myoblasts, GHR, IGF1