浙江农业学报 ›› 2022, Vol. 34 ›› Issue (4): 695-705.DOI: 10.3969/j.issn.1004-1524.2022.04.05

• 动物科学 • 上一篇    下一篇

卵形鲳鲹生肌调节因子基因家族的鉴定及在胚胎中的表达

余艳玲1(), 罗洪林1, 罗辉2, 冯鹏霏1, 潘传燕1, 宋漫玲1, 肖蕊1, 张永德1,*()   

  1. 1.广西水产科学研究院 广西水产遗传育种与健康养殖重点实验室,广西 南宁 530021
    2.西南大学 动物科学学院,重庆 400715
  • 收稿日期:2020-10-13 出版日期:2022-04-25 发布日期:2022-04-28
  • 通讯作者: 张永德
  • 作者简介:*张永德,E-mail: yondar@126.com
    余艳玲(1976—),女,陕西咸阳人,硕士,工程师,主要从事水产养殖研究。E-mail: 553850756@qq.com
  • 基金资助:
    广西创新驱动发展专项资金(桂科AA17204080-3);广西创新驱动发展专项资金(桂科AA18242031-2);广西水产遗传育种与康养殖重点实验室自主课题(17-A-01-02);广西水产遗传育种与康养殖重点实验室自主课题(19-A-01-05)

Genome-wide identification and expression of MRF gene family in embryonic development of Trachinotus ovatus

YU Yanling1(), LUO Honglin1, LUO Hui2, FENG Pengfei1, PAN Chuanyan1, SONG Manling1, XIAO Rui1, ZHANG Yongde1,*()   

  1. 1. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China
    2. College of Animal Science, Southwest University, Chongqing 400715, China
  • Received:2020-10-13 Online:2022-04-25 Published:2022-04-28
  • Contact: ZHANG Yongde

摘要:

为研究卵形鲳鲹生肌调节因子(MRF)在胚胎发育中所发挥的重要作用,对卵形鲳鲹MRF基因家族进行了全基因组鉴定和生物信息学分析,并对其在13个胚胎发育阶段的基因表达进行了定量分析。结果表明:在卵形鲳鲹基因组中共鉴定出5个MRF基因家族成员:MyoD1、MyoD2、Myf5、Myf6和MyoG,分别编码297、263、240、231、250个氨基酸。MRF家族基因存在典型的BASIC与HLH结构域,其中MyoD1、MyoD2与MyoG分别定位于9号、1号与2号染色体,而Myf5与Myf6基因均定位于22号染色体且位于同一基因座。MRF家族基因胚胎定量表达研究表明,MyoG在受精卵到胚体形成期表达量极低,在眼囊、耳囊与心脏跳动期表达量迅速升高,而到晶体出现期表达量又有所降低,推测其可能在眼囊、耳囊与心脏跳动期发挥主要的生肌调节作用,而后期在维持肌肉形态或肌肉发育中仍发挥重要作用。MyoD1、MyoD2、Myf5与Myf6在受精卵到原肠中期表达量较低,而从原肠末期到晶体出现期表达量迅速升高(P<0.05),表明其主要从原肠末期开始发挥生肌调控作用。结果表明,MRF家族成员在胚胎发育过程中发挥重要的作用,但不同的MRF家族成员发挥作用的时间及功能可能不同。

关键词: 卵形鲳鲹, 生肌调节因子(MRF), MRF基因家族, 基因表达, 胚胎发育

Abstract:

In order to study the important role of myogenic regulator (MRF) in the embryonic development of Trachinotus ovatus, the whole genome identification and bioinformatics analysis of MRF gene family in T. ovatus were carried out, and the gene expression in 13 embryonic stages was quantitatively analyzed. Five MRF gene family members, MyoD1, MyoD2, Myf5, Myf6 and MyoG, encoding 297, 263, 240, 231 and 250 amino acids, respectively, were identified in T. ovatus genome data. The MRF family genes had a typical BASIC and HLH domains, where MyoD1, MyoD2 and MyoG were located on chromosome 9, 1 and 2 respectively, while Myf5 and Myf6 were located on chromosome 22 and at the same locus. Quantitative expression studies in embryos showed that the expression level of MyoG was very low during the period from zygote to embryo formed stage, while increased rapidly during the optic vesicle stage, otocyst vesicle stage and heart pulsation stage, but decreased at the formation of eye lens stage. It was speculated that MyoG may play a major role in the regulation of myogenesis during optic vesicle stage, otocyst vesicle stage and heart pulsation stage, but still play an important role in maintaining muscle morphology or muscle development in the later period. The expression levels of MyoD1, MyoD2, Myf5 and Myf6 genes were lower from the zygote to the mid-gastrulation stage, but increased rapidly from the late gastrulation to the formation of eye lens stage (P<0.05), indicating that they began to play an important role in the regulation of myogenesis from late gastrulation stage. The results showed that MRF family members played an important role in the process of embryonic development of T. ovatus, but different MRF family members may play different roles in different time and functions.

Key words: Trachinotus ovatus, myogenic regulatory factors (MRF), MRF gene family, gene expression, embryo

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